Paralytic Toxins Accumulation and Tissue Expression of α-Amylase and Lipase Genes in the Pacific Oyster Crassostrea gigas Fed with the Neurotoxic Dinoflagellate Alexandrium catenella
The pacific oyster Crassostrea gigas was experimentally exposed to the neurotoxic Alexandrium catenella and a non-producer of PSTs, Alexandrium tamarense (control algae), at concentrations corresponding to those observed during the blooming period. At fixed time intervals, from 0 to 48 h, we determi...
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ftmdpi:oai:mdpi.com:/1660-3397/10/11/2519/ 2023-08-20T04:06:01+02:00 Paralytic Toxins Accumulation and Tissue Expression of α-Amylase and Lipase Genes in the Pacific Oyster Crassostrea gigas Fed with the Neurotoxic Dinoflagellate Alexandrium catenella Jean-Luc Rolland Kevin Pelletier Estelle Masseret Fabien Rieuvilleneuve Veronique Savar Adrien Santini Zouher Amzil Mohamed Laabir agris 2012-11-12 application/pdf https://doi.org/10.3390/md10112519 EN eng Multidisciplinary Digital Publishing Institute https://dx.doi.org/10.3390/md10112519 https://creativecommons.org/licenses/by/3.0/ Marine Drugs; Volume 10; Issue 11; Pages: 2519-2534 Crassostrea gigas Alexandrium catenella PSP toxins digestion gene expression Text 2012 ftmdpi https://doi.org/10.3390/md10112519 2023-07-31T20:30:36Z The pacific oyster Crassostrea gigas was experimentally exposed to the neurotoxic Alexandrium catenella and a non-producer of PSTs, Alexandrium tamarense (control algae), at concentrations corresponding to those observed during the blooming period. At fixed time intervals, from 0 to 48 h, we determined the clearance rate, the total filtered cells, the composition of the fecal ribbons, the profile of the PSP toxins and the variation of the expression of two α-amylase and triacylglecerol lipase precursor (TLP) genes through semi-quantitative RT-PCR. The results showed a significant decrease of the clearance rate of C. gigas fed with both Alexandrium species. However, from 29 to 48 h, the clearance rate and cell filtration activity increased only in oysters fed with A. tamarense. The toxin concentrations in the digestive gland rose above the sanitary threshold in less than 48 h of exposure and GTX6, a compound absent in A. catenella cells, accumulated. The α-amylase B gene expression level increased significantly in the time interval from 6 to 48 h in the digestive gland of oysters fed with A. tamarense, whereas the TLP gene transcript was significantly up-regulated in the digestive gland of oysters fed with the neurotoxic A. catenella. All together, these results suggest that the digestion capacity could be affected by PSP toxins. Text Crassostrea gigas Pacific oyster MDPI Open Access Publishing Pacific Marine Drugs 10 12 2519 2534 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
Crassostrea gigas Alexandrium catenella PSP toxins digestion gene expression |
spellingShingle |
Crassostrea gigas Alexandrium catenella PSP toxins digestion gene expression Jean-Luc Rolland Kevin Pelletier Estelle Masseret Fabien Rieuvilleneuve Veronique Savar Adrien Santini Zouher Amzil Mohamed Laabir Paralytic Toxins Accumulation and Tissue Expression of α-Amylase and Lipase Genes in the Pacific Oyster Crassostrea gigas Fed with the Neurotoxic Dinoflagellate Alexandrium catenella |
topic_facet |
Crassostrea gigas Alexandrium catenella PSP toxins digestion gene expression |
description |
The pacific oyster Crassostrea gigas was experimentally exposed to the neurotoxic Alexandrium catenella and a non-producer of PSTs, Alexandrium tamarense (control algae), at concentrations corresponding to those observed during the blooming period. At fixed time intervals, from 0 to 48 h, we determined the clearance rate, the total filtered cells, the composition of the fecal ribbons, the profile of the PSP toxins and the variation of the expression of two α-amylase and triacylglecerol lipase precursor (TLP) genes through semi-quantitative RT-PCR. The results showed a significant decrease of the clearance rate of C. gigas fed with both Alexandrium species. However, from 29 to 48 h, the clearance rate and cell filtration activity increased only in oysters fed with A. tamarense. The toxin concentrations in the digestive gland rose above the sanitary threshold in less than 48 h of exposure and GTX6, a compound absent in A. catenella cells, accumulated. The α-amylase B gene expression level increased significantly in the time interval from 6 to 48 h in the digestive gland of oysters fed with A. tamarense, whereas the TLP gene transcript was significantly up-regulated in the digestive gland of oysters fed with the neurotoxic A. catenella. All together, these results suggest that the digestion capacity could be affected by PSP toxins. |
format |
Text |
author |
Jean-Luc Rolland Kevin Pelletier Estelle Masseret Fabien Rieuvilleneuve Veronique Savar Adrien Santini Zouher Amzil Mohamed Laabir |
author_facet |
Jean-Luc Rolland Kevin Pelletier Estelle Masseret Fabien Rieuvilleneuve Veronique Savar Adrien Santini Zouher Amzil Mohamed Laabir |
author_sort |
Jean-Luc Rolland |
title |
Paralytic Toxins Accumulation and Tissue Expression of α-Amylase and Lipase Genes in the Pacific Oyster Crassostrea gigas Fed with the Neurotoxic Dinoflagellate Alexandrium catenella |
title_short |
Paralytic Toxins Accumulation and Tissue Expression of α-Amylase and Lipase Genes in the Pacific Oyster Crassostrea gigas Fed with the Neurotoxic Dinoflagellate Alexandrium catenella |
title_full |
Paralytic Toxins Accumulation and Tissue Expression of α-Amylase and Lipase Genes in the Pacific Oyster Crassostrea gigas Fed with the Neurotoxic Dinoflagellate Alexandrium catenella |
title_fullStr |
Paralytic Toxins Accumulation and Tissue Expression of α-Amylase and Lipase Genes in the Pacific Oyster Crassostrea gigas Fed with the Neurotoxic Dinoflagellate Alexandrium catenella |
title_full_unstemmed |
Paralytic Toxins Accumulation and Tissue Expression of α-Amylase and Lipase Genes in the Pacific Oyster Crassostrea gigas Fed with the Neurotoxic Dinoflagellate Alexandrium catenella |
title_sort |
paralytic toxins accumulation and tissue expression of α-amylase and lipase genes in the pacific oyster crassostrea gigas fed with the neurotoxic dinoflagellate alexandrium catenella |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2012 |
url |
https://doi.org/10.3390/md10112519 |
op_coverage |
agris |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Marine Drugs; Volume 10; Issue 11; Pages: 2519-2534 |
op_relation |
https://dx.doi.org/10.3390/md10112519 |
op_rights |
https://creativecommons.org/licenses/by/3.0/ |
op_doi |
https://doi.org/10.3390/md10112519 |
container_title |
Marine Drugs |
container_volume |
10 |
container_issue |
12 |
container_start_page |
2519 |
op_container_end_page |
2534 |
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1774716924752560128 |