Cloning, Expression, and Functional Characterization of FUT1, a Key Gene for Histo-Blood Group Antigens Synthesis in Crassostrea gigas
Histo-blood group antigens (HBGAs) comprise a family of cell-surface carbohydrates that are considered norovirus-specific binding receptors or ligands. HBGA-like molecules have also been detected in oysters as common norovirus carriers, although the pathway involved in the synthesis of these molecul...
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ftmdpi:oai:mdpi.com:/1467-3045/45/5/267/ 2023-08-20T04:06:01+02:00 Cloning, Expression, and Functional Characterization of FUT1, a Key Gene for Histo-Blood Group Antigens Synthesis in Crassostrea gigas Binbin Gui Lin Yao Meng Qu Weiran Zhang Mingyu Li Yanhua Jiang Lianzhu Wang agris 2023-05-09 application/pdf https://doi.org/10.3390/cimb45050267 EN eng Multidisciplinary Digital Publishing Institute Biochemistry, Molecular and Cellular Biology https://dx.doi.org/10.3390/cimb45050267 https://creativecommons.org/licenses/by/4.0/ Current Issues in Molecular Biology; Volume 45; Issue 5; Pages: 4200-4213 Crassostrea gigas norovirus HBGAs gene expression CgFUT1 Text 2023 ftmdpi https://doi.org/10.3390/cimb45050267 2023-08-01T10:00:15Z Histo-blood group antigens (HBGAs) comprise a family of cell-surface carbohydrates that are considered norovirus-specific binding receptors or ligands. HBGA-like molecules have also been detected in oysters as common norovirus carriers, although the pathway involved in the synthesis of these molecules in oysters has yet to be elucidated. We isolated and identified a key gene involved in the synthesis of HBGA-like molecules, FUT1, from Crassostrea gigas, named CgFUT1. Real-time quantitative polymerase chain reaction analysis showed that CgFUT1 mRNA was expressed in the mantle, gill, muscle, labellum, and hepatopancreatic tissues of C. gigas, with the hepatopancreas exhibiting the highest expression level. A recombinant CgFUT1 protein with a molecular mass of 38.0 kDa was expressed in Escherichia coli using a prokaryotic expression vector. A eukaryotic expression plasmid was constructed and transfected into Chinese hamster ovary (CHO) cells. The expression of CgFUT1 and membrane localization of type H-2 HBGA-like molecules in CHO cells were detected using Western blotting and cellular immunofluorescence, respectively. This study indicated that CgFUT1, expressed in C. gigas tissues, can synthesize type H-2 HBGA-like molecules. This finding provides a new perspective for analyzing the source and synthetic pathway of HBGA-like molecules in oysters. Text Crassostrea gigas MDPI Open Access Publishing Current Issues in Molecular Biology 45 5 4200 4213 |
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MDPI Open Access Publishing |
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English |
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Crassostrea gigas norovirus HBGAs gene expression CgFUT1 |
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Crassostrea gigas norovirus HBGAs gene expression CgFUT1 Binbin Gui Lin Yao Meng Qu Weiran Zhang Mingyu Li Yanhua Jiang Lianzhu Wang Cloning, Expression, and Functional Characterization of FUT1, a Key Gene for Histo-Blood Group Antigens Synthesis in Crassostrea gigas |
topic_facet |
Crassostrea gigas norovirus HBGAs gene expression CgFUT1 |
description |
Histo-blood group antigens (HBGAs) comprise a family of cell-surface carbohydrates that are considered norovirus-specific binding receptors or ligands. HBGA-like molecules have also been detected in oysters as common norovirus carriers, although the pathway involved in the synthesis of these molecules in oysters has yet to be elucidated. We isolated and identified a key gene involved in the synthesis of HBGA-like molecules, FUT1, from Crassostrea gigas, named CgFUT1. Real-time quantitative polymerase chain reaction analysis showed that CgFUT1 mRNA was expressed in the mantle, gill, muscle, labellum, and hepatopancreatic tissues of C. gigas, with the hepatopancreas exhibiting the highest expression level. A recombinant CgFUT1 protein with a molecular mass of 38.0 kDa was expressed in Escherichia coli using a prokaryotic expression vector. A eukaryotic expression plasmid was constructed and transfected into Chinese hamster ovary (CHO) cells. The expression of CgFUT1 and membrane localization of type H-2 HBGA-like molecules in CHO cells were detected using Western blotting and cellular immunofluorescence, respectively. This study indicated that CgFUT1, expressed in C. gigas tissues, can synthesize type H-2 HBGA-like molecules. This finding provides a new perspective for analyzing the source and synthetic pathway of HBGA-like molecules in oysters. |
format |
Text |
author |
Binbin Gui Lin Yao Meng Qu Weiran Zhang Mingyu Li Yanhua Jiang Lianzhu Wang |
author_facet |
Binbin Gui Lin Yao Meng Qu Weiran Zhang Mingyu Li Yanhua Jiang Lianzhu Wang |
author_sort |
Binbin Gui |
title |
Cloning, Expression, and Functional Characterization of FUT1, a Key Gene for Histo-Blood Group Antigens Synthesis in Crassostrea gigas |
title_short |
Cloning, Expression, and Functional Characterization of FUT1, a Key Gene for Histo-Blood Group Antigens Synthesis in Crassostrea gigas |
title_full |
Cloning, Expression, and Functional Characterization of FUT1, a Key Gene for Histo-Blood Group Antigens Synthesis in Crassostrea gigas |
title_fullStr |
Cloning, Expression, and Functional Characterization of FUT1, a Key Gene for Histo-Blood Group Antigens Synthesis in Crassostrea gigas |
title_full_unstemmed |
Cloning, Expression, and Functional Characterization of FUT1, a Key Gene for Histo-Blood Group Antigens Synthesis in Crassostrea gigas |
title_sort |
cloning, expression, and functional characterization of fut1, a key gene for histo-blood group antigens synthesis in crassostrea gigas |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2023 |
url |
https://doi.org/10.3390/cimb45050267 |
op_coverage |
agris |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Current Issues in Molecular Biology; Volume 45; Issue 5; Pages: 4200-4213 |
op_relation |
Biochemistry, Molecular and Cellular Biology https://dx.doi.org/10.3390/cimb45050267 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/cimb45050267 |
container_title |
Current Issues in Molecular Biology |
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45 |
container_issue |
5 |
container_start_page |
4200 |
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4213 |
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1774716919983636480 |