Graphene-Based and Surface-Enhanced Raman Spectroscopy for Monitoring the Physio-Chemical Response of Thermophilic Bacterial Spores to Low Temperatures Exposure
Monitoring the spore life cycle is one of the main issues in several fields including environmental control, sustainable ecosystems, food security, and healthcare systems. In this framework, the study of the living organism resistance to extreme conditions like those mimicking space environments is...
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ftmdpi:oai:mdpi.com:/1424-8220/20/15/4150/ 2023-08-20T04:02:31+02:00 Graphene-Based and Surface-Enhanced Raman Spectroscopy for Monitoring the Physio-Chemical Response of Thermophilic Bacterial Spores to Low Temperatures Exposure Carlo Camerlingo Giuseppe Di Meo Maria Lepore Mikhail Lisitskiy Annarita Poli Marianna Portaccio Ida Romano Paola Di Donato 2020-07-26 application/pdf https://doi.org/10.3390/s20154150 EN eng Multidisciplinary Digital Publishing Institute https://dx.doi.org/10.3390/s20154150 https://creativecommons.org/licenses/by/4.0/ Sensors; Volume 20; Issue 15; Pages: 4150 thermophilic bacteria spore germination SERS graphene-based pH-meter Text 2020 ftmdpi https://doi.org/10.3390/s20154150 2023-07-31T23:49:54Z Monitoring the spore life cycle is one of the main issues in several fields including environmental control, sustainable ecosystems, food security, and healthcare systems. In this framework, the study of the living organism resistance to extreme conditions like those mimicking space environments is particularly interesting. The assessment of the local change of the pH level can be extremely useful for this purpose. An optical physiometer method based on the Raman response of the graphene, which is able to locally sense pH of a fluid on a micrometric scale, has been recently proposed. Due to the presence of π -bonds at the surface, the electronic doping of graphene is determined by the external conditions and can be electrochemically controlled or altered by the contact with an acid or alkaline fluid. The doping level affects the vibrational energies of the graphene that can be monitored by conventional Raman spectroscopy. In addition, Surface-Enhanced Raman Spectroscopy (SERS) can give direct information on the biochemical changes occurring in spore components. In this work, we propose the joint use of Graphene-Based Raman Spectroscopy (GbRS) and SERS for the monitoring of the response of spores to exposure to low temperatures down to 100 K. The spores of the thermophilic bacterium Parageobacillus thermantarcticus isolated from an active volcano of Antarctica (Mt. Melbourne) were investigated. These spores are particularly resistant to several stressing stimuli and able to adapt to extreme conditions like low temperatures, UV irradiation, and γ -rays exposure. The results obtained showed that the joint use of GbRS and SERS represents a valuable tool for monitoring the physio-chemical response of bacterial spores upon exposure to stressing stimuli. Text Antarc* Antarctica MDPI Open Access Publishing Sensors 20 15 4150 |
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Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
thermophilic bacteria spore germination SERS graphene-based pH-meter |
spellingShingle |
thermophilic bacteria spore germination SERS graphene-based pH-meter Carlo Camerlingo Giuseppe Di Meo Maria Lepore Mikhail Lisitskiy Annarita Poli Marianna Portaccio Ida Romano Paola Di Donato Graphene-Based and Surface-Enhanced Raman Spectroscopy for Monitoring the Physio-Chemical Response of Thermophilic Bacterial Spores to Low Temperatures Exposure |
topic_facet |
thermophilic bacteria spore germination SERS graphene-based pH-meter |
description |
Monitoring the spore life cycle is one of the main issues in several fields including environmental control, sustainable ecosystems, food security, and healthcare systems. In this framework, the study of the living organism resistance to extreme conditions like those mimicking space environments is particularly interesting. The assessment of the local change of the pH level can be extremely useful for this purpose. An optical physiometer method based on the Raman response of the graphene, which is able to locally sense pH of a fluid on a micrometric scale, has been recently proposed. Due to the presence of π -bonds at the surface, the electronic doping of graphene is determined by the external conditions and can be electrochemically controlled or altered by the contact with an acid or alkaline fluid. The doping level affects the vibrational energies of the graphene that can be monitored by conventional Raman spectroscopy. In addition, Surface-Enhanced Raman Spectroscopy (SERS) can give direct information on the biochemical changes occurring in spore components. In this work, we propose the joint use of Graphene-Based Raman Spectroscopy (GbRS) and SERS for the monitoring of the response of spores to exposure to low temperatures down to 100 K. The spores of the thermophilic bacterium Parageobacillus thermantarcticus isolated from an active volcano of Antarctica (Mt. Melbourne) were investigated. These spores are particularly resistant to several stressing stimuli and able to adapt to extreme conditions like low temperatures, UV irradiation, and γ -rays exposure. The results obtained showed that the joint use of GbRS and SERS represents a valuable tool for monitoring the physio-chemical response of bacterial spores upon exposure to stressing stimuli. |
format |
Text |
author |
Carlo Camerlingo Giuseppe Di Meo Maria Lepore Mikhail Lisitskiy Annarita Poli Marianna Portaccio Ida Romano Paola Di Donato |
author_facet |
Carlo Camerlingo Giuseppe Di Meo Maria Lepore Mikhail Lisitskiy Annarita Poli Marianna Portaccio Ida Romano Paola Di Donato |
author_sort |
Carlo Camerlingo |
title |
Graphene-Based and Surface-Enhanced Raman Spectroscopy for Monitoring the Physio-Chemical Response of Thermophilic Bacterial Spores to Low Temperatures Exposure |
title_short |
Graphene-Based and Surface-Enhanced Raman Spectroscopy for Monitoring the Physio-Chemical Response of Thermophilic Bacterial Spores to Low Temperatures Exposure |
title_full |
Graphene-Based and Surface-Enhanced Raman Spectroscopy for Monitoring the Physio-Chemical Response of Thermophilic Bacterial Spores to Low Temperatures Exposure |
title_fullStr |
Graphene-Based and Surface-Enhanced Raman Spectroscopy for Monitoring the Physio-Chemical Response of Thermophilic Bacterial Spores to Low Temperatures Exposure |
title_full_unstemmed |
Graphene-Based and Surface-Enhanced Raman Spectroscopy for Monitoring the Physio-Chemical Response of Thermophilic Bacterial Spores to Low Temperatures Exposure |
title_sort |
graphene-based and surface-enhanced raman spectroscopy for monitoring the physio-chemical response of thermophilic bacterial spores to low temperatures exposure |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2020 |
url |
https://doi.org/10.3390/s20154150 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Sensors; Volume 20; Issue 15; Pages: 4150 |
op_relation |
https://dx.doi.org/10.3390/s20154150 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/s20154150 |
container_title |
Sensors |
container_volume |
20 |
container_issue |
15 |
container_start_page |
4150 |
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1774713000166424576 |