Appraisal of the Genus Pleurastrum (Chlorophyta) Based on Molecular and Climate Data
Two green microalgal strains, CCCryo 469-16 and CCCryo 470-16, were isolated from samples of Antarctic microflora. Their morphology and 18S rRNA sequences indicated a phylogenetic relationship with strains of the genera Chlorococcum Meneghini, Macrochloris Korshikov, and Pleurastrum Chodat. The last...
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ftmdpi:oai:mdpi.com:/1424-2818/15/5/650/ 2023-08-20T04:02:22+02:00 Appraisal of the Genus Pleurastrum (Chlorophyta) Based on Molecular and Climate Data Katia Sciuto Marion A. Wolf Michele Mistri Isabella Moro agris 2023-05-10 application/pdf https://doi.org/10.3390/d15050650 EN eng Multidisciplinary Digital Publishing Institute Phylogeny and Evolution https://dx.doi.org/10.3390/d15050650 https://creativecommons.org/licenses/by/4.0/ Diversity; Volume 15; Issue 5; Pages: 650 18S rRNA Chlorococcum climate zones ITS2 secondary structure Macrochloris rubrioleum Pleurastrum rbc L tuf A Text 2023 ftmdpi https://doi.org/10.3390/d15050650 2023-08-01T10:01:35Z Two green microalgal strains, CCCryo 469-16 and CCCryo 470-16, were isolated from samples of Antarctic microflora. Their morphology and 18S rRNA sequences indicated a phylogenetic relationship with strains of the genera Chlorococcum Meneghini, Macrochloris Korshikov, and Pleurastrum Chodat. The last genus is considered particularly problematic as it underwent several revisions. Moreover, its type strain, P. insigne SAG 30.93, was recently demonstrated to coincide genetically with the authentic strain of Chlorococcum oleofaciens from an 18S rRNA phylogeny. This deprived the genus Pleurastrum of an important type reference. Thus, the molecular markers rbcL, tufA, and ITS were employed to identify the Antarctic isolates more precisely. Several other microalgae related to our isolates were obtained from international culture collections for comparison. The obtained results allowed the re-establishment of strain P. insigne SAG 30.93 as the reference strain and the definition of the molecular borders of both genera Pleurastrum and Chlorococcum. Based on our findings, several Chlorococcum species are now re-attributed to Pleurastrum, as well as Macrochloris rubrioleum, here re-named Pleurastrum rubrioleum comb. nov., to which the Antarctic isolates also belong. Finally, a relationship between Pleurastrum lineages and climate zones was established. Text Antarc* Antarctic MDPI Open Access Publishing Antarctic The Antarctic Diversity 15 5 650 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
18S rRNA Chlorococcum climate zones ITS2 secondary structure Macrochloris rubrioleum Pleurastrum rbc L tuf A |
spellingShingle |
18S rRNA Chlorococcum climate zones ITS2 secondary structure Macrochloris rubrioleum Pleurastrum rbc L tuf A Katia Sciuto Marion A. Wolf Michele Mistri Isabella Moro Appraisal of the Genus Pleurastrum (Chlorophyta) Based on Molecular and Climate Data |
topic_facet |
18S rRNA Chlorococcum climate zones ITS2 secondary structure Macrochloris rubrioleum Pleurastrum rbc L tuf A |
description |
Two green microalgal strains, CCCryo 469-16 and CCCryo 470-16, were isolated from samples of Antarctic microflora. Their morphology and 18S rRNA sequences indicated a phylogenetic relationship with strains of the genera Chlorococcum Meneghini, Macrochloris Korshikov, and Pleurastrum Chodat. The last genus is considered particularly problematic as it underwent several revisions. Moreover, its type strain, P. insigne SAG 30.93, was recently demonstrated to coincide genetically with the authentic strain of Chlorococcum oleofaciens from an 18S rRNA phylogeny. This deprived the genus Pleurastrum of an important type reference. Thus, the molecular markers rbcL, tufA, and ITS were employed to identify the Antarctic isolates more precisely. Several other microalgae related to our isolates were obtained from international culture collections for comparison. The obtained results allowed the re-establishment of strain P. insigne SAG 30.93 as the reference strain and the definition of the molecular borders of both genera Pleurastrum and Chlorococcum. Based on our findings, several Chlorococcum species are now re-attributed to Pleurastrum, as well as Macrochloris rubrioleum, here re-named Pleurastrum rubrioleum comb. nov., to which the Antarctic isolates also belong. Finally, a relationship between Pleurastrum lineages and climate zones was established. |
format |
Text |
author |
Katia Sciuto Marion A. Wolf Michele Mistri Isabella Moro |
author_facet |
Katia Sciuto Marion A. Wolf Michele Mistri Isabella Moro |
author_sort |
Katia Sciuto |
title |
Appraisal of the Genus Pleurastrum (Chlorophyta) Based on Molecular and Climate Data |
title_short |
Appraisal of the Genus Pleurastrum (Chlorophyta) Based on Molecular and Climate Data |
title_full |
Appraisal of the Genus Pleurastrum (Chlorophyta) Based on Molecular and Climate Data |
title_fullStr |
Appraisal of the Genus Pleurastrum (Chlorophyta) Based on Molecular and Climate Data |
title_full_unstemmed |
Appraisal of the Genus Pleurastrum (Chlorophyta) Based on Molecular and Climate Data |
title_sort |
appraisal of the genus pleurastrum (chlorophyta) based on molecular and climate data |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2023 |
url |
https://doi.org/10.3390/d15050650 |
op_coverage |
agris |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Diversity; Volume 15; Issue 5; Pages: 650 |
op_relation |
Phylogeny and Evolution https://dx.doi.org/10.3390/d15050650 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/d15050650 |
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Diversity |
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15 |
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5 |
container_start_page |
650 |
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