Characterization of Terrihabitans soli gen. nov., sp. nov., a Novel 0.2 μm-Filterable Soil Bacterium Belonging to a Widely Distributed Lineage of Hyphomicrobiales (Rhizobiales)
We previously showed that novel filterable bacteria remain in “sterile” (<0.2 μm filtered) terrestrial environmental samples from Japan, China, and Arctic Norway. Here, we characterized the novel filterable strain IZ6T, a representative strain of a widely distributed lineage. Phylogenetic analysi...
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ftmdpi:oai:mdpi.com:/1424-2818/13/9/422/ 2023-08-20T04:04:43+02:00 Characterization of Terrihabitans soli gen. nov., sp. nov., a Novel 0.2 μm-Filterable Soil Bacterium Belonging to a Widely Distributed Lineage of Hyphomicrobiales (Rhizobiales) Ryosuke Nakai Takeshi Naganuma Nozomi Tazato Tadao Kunihiro Sho Morohoshi Tomomi Koide Hiroyuki Kusada Hideyuki Tamaki Takashi Narihiro agris 2021-09-01 application/pdf https://doi.org/10.3390/d13090422 EN eng Multidisciplinary Digital Publishing Institute Microbial Diversity and Culture Collections https://dx.doi.org/10.3390/d13090422 https://creativecommons.org/licenses/by/4.0/ Diversity; Volume 13; Issue 9; Pages: 422 filter sterilization filterable bacteria soil phylogeny taxonomy Hyphomicrobiales Rhizobiales Text 2021 ftmdpi https://doi.org/10.3390/d13090422 2023-08-01T02:35:32Z We previously showed that novel filterable bacteria remain in “sterile” (<0.2 μm filtered) terrestrial environmental samples from Japan, China, and Arctic Norway. Here, we characterized the novel filterable strain IZ6T, a representative strain of a widely distributed lineage. Phylogenetic analysis showed that this strain was affiliated with the Rhizobiales (now proposed as Hyphomicrobiales) of Alphaproteobacteria, but distinct from any other type strains. Strain IZ6T shared the following chemotaxonomic features with the closest (but distantly) related type strain, Flaviflagellibacter deserti SYSU D60017T: ubiquinone-10 as the major quinone; phosphatidylethanolamine, phosphatidylcholine, and phosphatidylglycerol as major polar lipids; and slightly high G+C content of 62.2 mol%. However, the cellular fatty acid composition differed between them, and the unsaturated fatty acid (C18:1ω7c/C18:1ω6c) was predominantly found in our strain. Moreover, unlike methyrotrophs and nitrogen-fixers of the neighboring genera of Hyphomicrobiales (Rhizobiales), strain IZ6T cannot utilize a one-carbon compound (e.g., methanol) and fix atmospheric nitrogen gas. These findings were consistent with the genome-inferred physiological potential. Based on the phylogenetic, physiological, and chemotaxonomic traits, we propose that strain IZ6T represents a novel genus and species with the name Terrihabitans soli gen. nov., sp. nov. (=NBRC 106741T = NCIMB 15058T). The findings will provide deeper insight into the eco-physiology of filterable microorganisms. Text Arctic MDPI Open Access Publishing Arctic Norway Diversity 13 9 422 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
filter sterilization filterable bacteria soil phylogeny taxonomy Hyphomicrobiales Rhizobiales |
spellingShingle |
filter sterilization filterable bacteria soil phylogeny taxonomy Hyphomicrobiales Rhizobiales Ryosuke Nakai Takeshi Naganuma Nozomi Tazato Tadao Kunihiro Sho Morohoshi Tomomi Koide Hiroyuki Kusada Hideyuki Tamaki Takashi Narihiro Characterization of Terrihabitans soli gen. nov., sp. nov., a Novel 0.2 μm-Filterable Soil Bacterium Belonging to a Widely Distributed Lineage of Hyphomicrobiales (Rhizobiales) |
topic_facet |
filter sterilization filterable bacteria soil phylogeny taxonomy Hyphomicrobiales Rhizobiales |
description |
We previously showed that novel filterable bacteria remain in “sterile” (<0.2 μm filtered) terrestrial environmental samples from Japan, China, and Arctic Norway. Here, we characterized the novel filterable strain IZ6T, a representative strain of a widely distributed lineage. Phylogenetic analysis showed that this strain was affiliated with the Rhizobiales (now proposed as Hyphomicrobiales) of Alphaproteobacteria, but distinct from any other type strains. Strain IZ6T shared the following chemotaxonomic features with the closest (but distantly) related type strain, Flaviflagellibacter deserti SYSU D60017T: ubiquinone-10 as the major quinone; phosphatidylethanolamine, phosphatidylcholine, and phosphatidylglycerol as major polar lipids; and slightly high G+C content of 62.2 mol%. However, the cellular fatty acid composition differed between them, and the unsaturated fatty acid (C18:1ω7c/C18:1ω6c) was predominantly found in our strain. Moreover, unlike methyrotrophs and nitrogen-fixers of the neighboring genera of Hyphomicrobiales (Rhizobiales), strain IZ6T cannot utilize a one-carbon compound (e.g., methanol) and fix atmospheric nitrogen gas. These findings were consistent with the genome-inferred physiological potential. Based on the phylogenetic, physiological, and chemotaxonomic traits, we propose that strain IZ6T represents a novel genus and species with the name Terrihabitans soli gen. nov., sp. nov. (=NBRC 106741T = NCIMB 15058T). The findings will provide deeper insight into the eco-physiology of filterable microorganisms. |
format |
Text |
author |
Ryosuke Nakai Takeshi Naganuma Nozomi Tazato Tadao Kunihiro Sho Morohoshi Tomomi Koide Hiroyuki Kusada Hideyuki Tamaki Takashi Narihiro |
author_facet |
Ryosuke Nakai Takeshi Naganuma Nozomi Tazato Tadao Kunihiro Sho Morohoshi Tomomi Koide Hiroyuki Kusada Hideyuki Tamaki Takashi Narihiro |
author_sort |
Ryosuke Nakai |
title |
Characterization of Terrihabitans soli gen. nov., sp. nov., a Novel 0.2 μm-Filterable Soil Bacterium Belonging to a Widely Distributed Lineage of Hyphomicrobiales (Rhizobiales) |
title_short |
Characterization of Terrihabitans soli gen. nov., sp. nov., a Novel 0.2 μm-Filterable Soil Bacterium Belonging to a Widely Distributed Lineage of Hyphomicrobiales (Rhizobiales) |
title_full |
Characterization of Terrihabitans soli gen. nov., sp. nov., a Novel 0.2 μm-Filterable Soil Bacterium Belonging to a Widely Distributed Lineage of Hyphomicrobiales (Rhizobiales) |
title_fullStr |
Characterization of Terrihabitans soli gen. nov., sp. nov., a Novel 0.2 μm-Filterable Soil Bacterium Belonging to a Widely Distributed Lineage of Hyphomicrobiales (Rhizobiales) |
title_full_unstemmed |
Characterization of Terrihabitans soli gen. nov., sp. nov., a Novel 0.2 μm-Filterable Soil Bacterium Belonging to a Widely Distributed Lineage of Hyphomicrobiales (Rhizobiales) |
title_sort |
characterization of terrihabitans soli gen. nov., sp. nov., a novel 0.2 μm-filterable soil bacterium belonging to a widely distributed lineage of hyphomicrobiales (rhizobiales) |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2021 |
url |
https://doi.org/10.3390/d13090422 |
op_coverage |
agris |
geographic |
Arctic Norway |
geographic_facet |
Arctic Norway |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Diversity; Volume 13; Issue 9; Pages: 422 |
op_relation |
Microbial Diversity and Culture Collections https://dx.doi.org/10.3390/d13090422 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/d13090422 |
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Diversity |
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13 |
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9 |
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422 |
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