Bacterial Number and Genetic Diversity in a Permafrost Peatland (Western Siberia): Testing a Link with Organic Matter Quality and Elementary Composition of a Peat Soil Profile
Permafrost peatlands, containing a sizable amount of soil organic carbon (OC), play a pivotal role in soil (peat) OC transformation into soluble and volatile forms and greatly contribute to overall natural CO2 and CH4 emissions to the atmosphere under ongoing permafrost thaw and soil OC degradation....
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ftmdpi:oai:mdpi.com:/1424-2818/13/7/328/ 2023-08-20T04:07:07+02:00 Bacterial Number and Genetic Diversity in a Permafrost Peatland (Western Siberia): Testing a Link with Organic Matter Quality and Elementary Composition of a Peat Soil Profile Andrey S. Aksenov Liudmila S. Shirokova Oksana Ya. Kisil Sofiia N. Kolesova Artem G. Lim Darya Kuzmina Sophie Pouillé Marie A. Alexis Maryse Castrec-Rouelle Sergey V. Loiko Oleg S. Pokrovsky agris 2021-07-17 application/pdf https://doi.org/10.3390/d13070328 EN eng Multidisciplinary Digital Publishing Institute https://dx.doi.org/10.3390/d13070328 https://creativecommons.org/licenses/by/4.0/ Diversity; Volume 13; Issue 7; Pages: 328 permafrost peat pore water bacteria number metabolism active layer Text 2021 ftmdpi https://doi.org/10.3390/d13070328 2023-08-01T02:12:46Z Permafrost peatlands, containing a sizable amount of soil organic carbon (OC), play a pivotal role in soil (peat) OC transformation into soluble and volatile forms and greatly contribute to overall natural CO2 and CH4 emissions to the atmosphere under ongoing permafrost thaw and soil OC degradation. Peat microorganisms are largely responsible for the processing of this OC, yet coupled studies of chemical and bacterial parameters in permafrost peatlands are rather limited and geographically biased. Towards testing the possible impact of peat and peat pore water chemical composition on microbial population and diversity, here we present results of a preliminary study of the western Siberia permafrost peatland discontinuous permafrost zone. The quantitative evaluation of microorganisms and determination of microbial diversity along a 100 cm thick peat soil column, which included thawed and frozen peat and bottom mineral horizon, was performed by RT-PCR and 16S rRNA gene-based metagenomic analysis, respectively. Bacteria (mainly Proteobacteria, Acidobacteria, Actinobacteria) strongly dominated the microbial diversity (99% sequences), with a negligible proportion of archaea (0.3–0.5%). There was a systematic evolution of main taxa according to depth, with a maximum of 65% (Acidobacteria) encountered in the active layer, or permafrost boundary (50–60 cm). We also measured C, N, nutrients and ~50 major and trace elements in peat (19 samples) as well as its pore water and dispersed ice (10 samples), sampled over the same core, and we analyzed organic matter quality in six organic and one mineral horizon of this core. Using multiparametric statistics (PCA), we tested the links between the total microbial number and 16S rRNA diversity and chemical composition of both the solid and fluid phase harboring the microorganisms. Under climate warming and permafrost thaw, one can expect a downward movement of the layer of maximal genetic diversity following the active layer thickening. Given a one to two orders of magnitude ... Text Ice permafrost Siberia MDPI Open Access Publishing Diversity 13 7 328 |
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MDPI Open Access Publishing |
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English |
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permafrost peat pore water bacteria number metabolism active layer |
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permafrost peat pore water bacteria number metabolism active layer Andrey S. Aksenov Liudmila S. Shirokova Oksana Ya. Kisil Sofiia N. Kolesova Artem G. Lim Darya Kuzmina Sophie Pouillé Marie A. Alexis Maryse Castrec-Rouelle Sergey V. Loiko Oleg S. Pokrovsky Bacterial Number and Genetic Diversity in a Permafrost Peatland (Western Siberia): Testing a Link with Organic Matter Quality and Elementary Composition of a Peat Soil Profile |
topic_facet |
permafrost peat pore water bacteria number metabolism active layer |
description |
Permafrost peatlands, containing a sizable amount of soil organic carbon (OC), play a pivotal role in soil (peat) OC transformation into soluble and volatile forms and greatly contribute to overall natural CO2 and CH4 emissions to the atmosphere under ongoing permafrost thaw and soil OC degradation. Peat microorganisms are largely responsible for the processing of this OC, yet coupled studies of chemical and bacterial parameters in permafrost peatlands are rather limited and geographically biased. Towards testing the possible impact of peat and peat pore water chemical composition on microbial population and diversity, here we present results of a preliminary study of the western Siberia permafrost peatland discontinuous permafrost zone. The quantitative evaluation of microorganisms and determination of microbial diversity along a 100 cm thick peat soil column, which included thawed and frozen peat and bottom mineral horizon, was performed by RT-PCR and 16S rRNA gene-based metagenomic analysis, respectively. Bacteria (mainly Proteobacteria, Acidobacteria, Actinobacteria) strongly dominated the microbial diversity (99% sequences), with a negligible proportion of archaea (0.3–0.5%). There was a systematic evolution of main taxa according to depth, with a maximum of 65% (Acidobacteria) encountered in the active layer, or permafrost boundary (50–60 cm). We also measured C, N, nutrients and ~50 major and trace elements in peat (19 samples) as well as its pore water and dispersed ice (10 samples), sampled over the same core, and we analyzed organic matter quality in six organic and one mineral horizon of this core. Using multiparametric statistics (PCA), we tested the links between the total microbial number and 16S rRNA diversity and chemical composition of both the solid and fluid phase harboring the microorganisms. Under climate warming and permafrost thaw, one can expect a downward movement of the layer of maximal genetic diversity following the active layer thickening. Given a one to two orders of magnitude ... |
format |
Text |
author |
Andrey S. Aksenov Liudmila S. Shirokova Oksana Ya. Kisil Sofiia N. Kolesova Artem G. Lim Darya Kuzmina Sophie Pouillé Marie A. Alexis Maryse Castrec-Rouelle Sergey V. Loiko Oleg S. Pokrovsky |
author_facet |
Andrey S. Aksenov Liudmila S. Shirokova Oksana Ya. Kisil Sofiia N. Kolesova Artem G. Lim Darya Kuzmina Sophie Pouillé Marie A. Alexis Maryse Castrec-Rouelle Sergey V. Loiko Oleg S. Pokrovsky |
author_sort |
Andrey S. Aksenov |
title |
Bacterial Number and Genetic Diversity in a Permafrost Peatland (Western Siberia): Testing a Link with Organic Matter Quality and Elementary Composition of a Peat Soil Profile |
title_short |
Bacterial Number and Genetic Diversity in a Permafrost Peatland (Western Siberia): Testing a Link with Organic Matter Quality and Elementary Composition of a Peat Soil Profile |
title_full |
Bacterial Number and Genetic Diversity in a Permafrost Peatland (Western Siberia): Testing a Link with Organic Matter Quality and Elementary Composition of a Peat Soil Profile |
title_fullStr |
Bacterial Number and Genetic Diversity in a Permafrost Peatland (Western Siberia): Testing a Link with Organic Matter Quality and Elementary Composition of a Peat Soil Profile |
title_full_unstemmed |
Bacterial Number and Genetic Diversity in a Permafrost Peatland (Western Siberia): Testing a Link with Organic Matter Quality and Elementary Composition of a Peat Soil Profile |
title_sort |
bacterial number and genetic diversity in a permafrost peatland (western siberia): testing a link with organic matter quality and elementary composition of a peat soil profile |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2021 |
url |
https://doi.org/10.3390/d13070328 |
op_coverage |
agris |
genre |
Ice permafrost Siberia |
genre_facet |
Ice permafrost Siberia |
op_source |
Diversity; Volume 13; Issue 7; Pages: 328 |
op_relation |
https://dx.doi.org/10.3390/d13070328 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/d13070328 |
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Diversity |
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328 |
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