Statistical Assessment of Phenol Biodegradation by a Metal-Tolerant Binary Consortium of Indigenous Antarctic Bacteria
Since the heroic age of Antarctic exploration, the continent has been pressurized by multiple anthropogenic activities, today including research and tourism, which have led to the emergence of phenol pollution. Natural attenuation rates are very slow in this region due to the harsh environmental con...
Published in: | Diversity |
---|---|
Main Authors: | , , , , , , , |
Format: | Text |
Language: | English |
Published: |
Multidisciplinary Digital Publishing Institute
2021
|
Subjects: | |
Online Access: | https://doi.org/10.3390/d13120643 |
id |
ftmdpi:oai:mdpi.com:/1424-2818/13/12/643/ |
---|---|
record_format |
openpolar |
spelling |
ftmdpi:oai:mdpi.com:/1424-2818/13/12/643/ 2023-08-20T04:01:57+02:00 Statistical Assessment of Phenol Biodegradation by a Metal-Tolerant Binary Consortium of Indigenous Antarctic Bacteria Kavilasni Subramaniam Siti Aqlima Ahmad Peter Convey Noor Azmi Shaharuddin Khalilah Abdul Khalil Tengku Athirrah Tengku-Mazuki Claudio Gomez-Fuentes Azham Zulkharnain agris 2021-12-04 application/pdf https://doi.org/10.3390/d13120643 EN eng Multidisciplinary Digital Publishing Institute Microbial Diversity and Culture Collections https://dx.doi.org/10.3390/d13120643 https://creativecommons.org/licenses/by/4.0/ Diversity; Volume 13; Issue 12; Pages: 643 cold climate pollution statistical optimization mixed culture metal ion Text 2021 ftmdpi https://doi.org/10.3390/d13120643 2023-08-01T03:27:43Z Since the heroic age of Antarctic exploration, the continent has been pressurized by multiple anthropogenic activities, today including research and tourism, which have led to the emergence of phenol pollution. Natural attenuation rates are very slow in this region due to the harsh environmental conditions; hence, biodegradation of phenol using native bacterial strains is recognized as a sustainable remediation approach. The aim of this study was to analyze the effectiveness of phenol degradation by a binary consortium of Antarctic soil bacteria, Arthrobacter sp. strain AQ5-06, and Arthrobacter sp. strain AQ5-15. Phenol degradation by this co-culture was statistically optimized using response surface methodology (RSM) and tolerance of exposure to different heavy metals was investigated under optimized conditions. Analysis of variance of central composite design (CCD) identified temperature as the most significant factor that affects phenol degradation by this consortium, with the optimum temperature ranging from 12.50 to 13.75 °C. This co-culture was able to degrade up to 1.7 g/L of phenol within seven days and tolerated phenol concentration as high as 1.9 g/L. Investigation of heavy metal tolerance revealed phenol biodegradation by this co-culture was completed in the presence of arsenic (As), aluminum (Al), copper (Cu), zinc (Zn), lead (Pb), cobalt (Co), chromium (Cr), and nickel (Ni) at concentrations of 1.0 ppm, but was inhibited by cadmium (Cd), silver (Ag), and mercury (Hg). Text Antarc* Antarctic MDPI Open Access Publishing Antarctic Diversity 13 12 643 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
cold climate pollution statistical optimization mixed culture metal ion |
spellingShingle |
cold climate pollution statistical optimization mixed culture metal ion Kavilasni Subramaniam Siti Aqlima Ahmad Peter Convey Noor Azmi Shaharuddin Khalilah Abdul Khalil Tengku Athirrah Tengku-Mazuki Claudio Gomez-Fuentes Azham Zulkharnain Statistical Assessment of Phenol Biodegradation by a Metal-Tolerant Binary Consortium of Indigenous Antarctic Bacteria |
topic_facet |
cold climate pollution statistical optimization mixed culture metal ion |
description |
Since the heroic age of Antarctic exploration, the continent has been pressurized by multiple anthropogenic activities, today including research and tourism, which have led to the emergence of phenol pollution. Natural attenuation rates are very slow in this region due to the harsh environmental conditions; hence, biodegradation of phenol using native bacterial strains is recognized as a sustainable remediation approach. The aim of this study was to analyze the effectiveness of phenol degradation by a binary consortium of Antarctic soil bacteria, Arthrobacter sp. strain AQ5-06, and Arthrobacter sp. strain AQ5-15. Phenol degradation by this co-culture was statistically optimized using response surface methodology (RSM) and tolerance of exposure to different heavy metals was investigated under optimized conditions. Analysis of variance of central composite design (CCD) identified temperature as the most significant factor that affects phenol degradation by this consortium, with the optimum temperature ranging from 12.50 to 13.75 °C. This co-culture was able to degrade up to 1.7 g/L of phenol within seven days and tolerated phenol concentration as high as 1.9 g/L. Investigation of heavy metal tolerance revealed phenol biodegradation by this co-culture was completed in the presence of arsenic (As), aluminum (Al), copper (Cu), zinc (Zn), lead (Pb), cobalt (Co), chromium (Cr), and nickel (Ni) at concentrations of 1.0 ppm, but was inhibited by cadmium (Cd), silver (Ag), and mercury (Hg). |
format |
Text |
author |
Kavilasni Subramaniam Siti Aqlima Ahmad Peter Convey Noor Azmi Shaharuddin Khalilah Abdul Khalil Tengku Athirrah Tengku-Mazuki Claudio Gomez-Fuentes Azham Zulkharnain |
author_facet |
Kavilasni Subramaniam Siti Aqlima Ahmad Peter Convey Noor Azmi Shaharuddin Khalilah Abdul Khalil Tengku Athirrah Tengku-Mazuki Claudio Gomez-Fuentes Azham Zulkharnain |
author_sort |
Kavilasni Subramaniam |
title |
Statistical Assessment of Phenol Biodegradation by a Metal-Tolerant Binary Consortium of Indigenous Antarctic Bacteria |
title_short |
Statistical Assessment of Phenol Biodegradation by a Metal-Tolerant Binary Consortium of Indigenous Antarctic Bacteria |
title_full |
Statistical Assessment of Phenol Biodegradation by a Metal-Tolerant Binary Consortium of Indigenous Antarctic Bacteria |
title_fullStr |
Statistical Assessment of Phenol Biodegradation by a Metal-Tolerant Binary Consortium of Indigenous Antarctic Bacteria |
title_full_unstemmed |
Statistical Assessment of Phenol Biodegradation by a Metal-Tolerant Binary Consortium of Indigenous Antarctic Bacteria |
title_sort |
statistical assessment of phenol biodegradation by a metal-tolerant binary consortium of indigenous antarctic bacteria |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2021 |
url |
https://doi.org/10.3390/d13120643 |
op_coverage |
agris |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Diversity; Volume 13; Issue 12; Pages: 643 |
op_relation |
Microbial Diversity and Culture Collections https://dx.doi.org/10.3390/d13120643 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/d13120643 |
container_title |
Diversity |
container_volume |
13 |
container_issue |
12 |
container_start_page |
643 |
_version_ |
1774712328869117952 |