Blood Metabolite Profiling of Antarctic Expedition Members: An 1H NMR Spectroscopy-Based Study
Serum samples from eight participants during the XV winter-over at Concordia base (Antarctic expedition) collected at defined time points, including predeparture, constituted the key substrates for a specific metabolomics study. To ascertain acute changes and chronic adaptation to hypoxia, the metab...
Published in: | International Journal of Molecular Sciences |
---|---|
Main Authors: | , , , , , , , , , |
Format: | Text |
Language: | English |
Published: |
Multidisciplinary Digital Publishing Institute
2023
|
Subjects: | |
Online Access: | https://doi.org/10.3390/ijms24098459 |
id |
ftmdpi:oai:mdpi.com:/1422-0067/24/9/8459/ |
---|---|
record_format |
openpolar |
spelling |
ftmdpi:oai:mdpi.com:/1422-0067/24/9/8459/ 2023-08-20T04:00:03+02:00 Blood Metabolite Profiling of Antarctic Expedition Members: An 1H NMR Spectroscopy-Based Study Laura Del Coco Marco Greco Alessandra Inguscio Anas Munir Antonio Danieli Luca Cossa Debora Musarò Maria Rosaria Coscia Francesco Paolo Fanizzi Michele Maffia agris 2023-05-08 application/pdf https://doi.org/10.3390/ijms24098459 EN eng Multidisciplinary Digital Publishing Institute Molecular Endocrinology and Metabolism https://dx.doi.org/10.3390/ijms24098459 https://creativecommons.org/licenses/by/4.0/ International Journal of Molecular Sciences; Volume 24; Issue 9; Pages: 8459 hypoxia winter-over Antarctica Concordia base adaptation NMR metabolomics Text 2023 ftmdpi https://doi.org/10.3390/ijms24098459 2023-08-01T09:59:46Z Serum samples from eight participants during the XV winter-over at Concordia base (Antarctic expedition) collected at defined time points, including predeparture, constituted the key substrates for a specific metabolomics study. To ascertain acute changes and chronic adaptation to hypoxia, the metabolic profiles of the serum samples were analyzed using NMR spectroscopy, with principal components analysis (PCA) followed by partial least squares and orthogonal partial least squares discriminant analyses (PLS-DA and OPLS-DA) used as supervised classification methods. Multivariate data analyses clearly highlighted an adaptation period characterized by an increase in the levels of circulating glutamine and lipids, mobilized to supply the body energy needs. At the same time, a reduction in the circulating levels of glutamate and N-acetyl glycoproteins, stress condition indicators, and proinflammatory markers were also found in the NMR data investigation. Subsequent pathway analysis showed possible perturbations in metabolic processes, potentially related to the physiological adaptation, predominantly found by comparing the baseline (at sea level, before mission onset), the base arrival, and the mission ending collected values. Text Antarc* Antarctic Antarctica MDPI Open Access Publishing Antarctic International Journal of Molecular Sciences 24 9 8459 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
hypoxia winter-over Antarctica Concordia base adaptation NMR metabolomics |
spellingShingle |
hypoxia winter-over Antarctica Concordia base adaptation NMR metabolomics Laura Del Coco Marco Greco Alessandra Inguscio Anas Munir Antonio Danieli Luca Cossa Debora Musarò Maria Rosaria Coscia Francesco Paolo Fanizzi Michele Maffia Blood Metabolite Profiling of Antarctic Expedition Members: An 1H NMR Spectroscopy-Based Study |
topic_facet |
hypoxia winter-over Antarctica Concordia base adaptation NMR metabolomics |
description |
Serum samples from eight participants during the XV winter-over at Concordia base (Antarctic expedition) collected at defined time points, including predeparture, constituted the key substrates for a specific metabolomics study. To ascertain acute changes and chronic adaptation to hypoxia, the metabolic profiles of the serum samples were analyzed using NMR spectroscopy, with principal components analysis (PCA) followed by partial least squares and orthogonal partial least squares discriminant analyses (PLS-DA and OPLS-DA) used as supervised classification methods. Multivariate data analyses clearly highlighted an adaptation period characterized by an increase in the levels of circulating glutamine and lipids, mobilized to supply the body energy needs. At the same time, a reduction in the circulating levels of glutamate and N-acetyl glycoproteins, stress condition indicators, and proinflammatory markers were also found in the NMR data investigation. Subsequent pathway analysis showed possible perturbations in metabolic processes, potentially related to the physiological adaptation, predominantly found by comparing the baseline (at sea level, before mission onset), the base arrival, and the mission ending collected values. |
format |
Text |
author |
Laura Del Coco Marco Greco Alessandra Inguscio Anas Munir Antonio Danieli Luca Cossa Debora Musarò Maria Rosaria Coscia Francesco Paolo Fanizzi Michele Maffia |
author_facet |
Laura Del Coco Marco Greco Alessandra Inguscio Anas Munir Antonio Danieli Luca Cossa Debora Musarò Maria Rosaria Coscia Francesco Paolo Fanizzi Michele Maffia |
author_sort |
Laura Del Coco |
title |
Blood Metabolite Profiling of Antarctic Expedition Members: An 1H NMR Spectroscopy-Based Study |
title_short |
Blood Metabolite Profiling of Antarctic Expedition Members: An 1H NMR Spectroscopy-Based Study |
title_full |
Blood Metabolite Profiling of Antarctic Expedition Members: An 1H NMR Spectroscopy-Based Study |
title_fullStr |
Blood Metabolite Profiling of Antarctic Expedition Members: An 1H NMR Spectroscopy-Based Study |
title_full_unstemmed |
Blood Metabolite Profiling of Antarctic Expedition Members: An 1H NMR Spectroscopy-Based Study |
title_sort |
blood metabolite profiling of antarctic expedition members: an 1h nmr spectroscopy-based study |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2023 |
url |
https://doi.org/10.3390/ijms24098459 |
op_coverage |
agris |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_source |
International Journal of Molecular Sciences; Volume 24; Issue 9; Pages: 8459 |
op_relation |
Molecular Endocrinology and Metabolism https://dx.doi.org/10.3390/ijms24098459 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/ijms24098459 |
container_title |
International Journal of Molecular Sciences |
container_volume |
24 |
container_issue |
9 |
container_start_page |
8459 |
_version_ |
1774716553177071616 |