Participants in the Trans-Antarctic Winter Traverse Expedition Showed Increased Bacterial Load and Diversity in Saliva but Maintained Individual Differences within Stool Microbiota and Across Metabolite Fingerprints
Understanding the impact of long-term physiological and environmental stress on the human microbiota and metabolome may be important for the success of space flight. This work is logistically difficult and has a limited number of available participants. Terrestrial analogies present important opport...
Published in: | International Journal of Molecular Sciences |
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Online Access: | https://doi.org/10.3390/ijms24054850 |
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ftmdpi:oai:mdpi.com:/1422-0067/24/5/4850/ 2023-08-20T04:01:05+02:00 Participants in the Trans-Antarctic Winter Traverse Expedition Showed Increased Bacterial Load and Diversity in Saliva but Maintained Individual Differences within Stool Microbiota and Across Metabolite Fingerprints Simon J. S. Cameron Arwyn Edwards Robert J. Lambert Mike Stroud Luis A. J. Mur agris 2023-03-02 application/pdf https://doi.org/10.3390/ijms24054850 EN eng Multidisciplinary Digital Publishing Institute Molecular Microbiology https://dx.doi.org/10.3390/ijms24054850 https://creativecommons.org/licenses/by/4.0/ International Journal of Molecular Sciences; Volume 24; Issue 5; Pages: 4850 salivary microbiota stool microbiota metabolomics metabolite fingerprinting isolation longitudinal microbiota dynamics microbiota-metabolome integration Text 2023 ftmdpi https://doi.org/10.3390/ijms24054850 2023-08-01T09:04:56Z Understanding the impact of long-term physiological and environmental stress on the human microbiota and metabolome may be important for the success of space flight. This work is logistically difficult and has a limited number of available participants. Terrestrial analogies present important opportunities to understand changes in the microbiota and metabolome and how this may impact participant health and fitness. Here, we present work from one such analogy: the Transarctic Winter Traverse expedition, which we believe is the first assessment of the microbiota and metabolome from different bodily locations during prolonged environmental and physiological stress. Bacterial load and diversity were significantly higher during the expedition when compared with baseline levels (p < 0.001) in saliva but not stool, and only a single operational taxonomic unit assigned to the Ruminococcaceae family shows significantly altered levels in stool (p < 0.001). Metabolite fingerprints show the maintenance of individual differences across saliva, stool, and plasma samples when analysed using flow infusion electrospray mass spectrometry and Fourier transform infrared spectroscopy. Significant activity-associated changes in terms of both bacterial diversity and load are seen in saliva but not in stool, and participant differences in metabolite fingerprints persist across all three sample types. Text Antarc* Antarctic MDPI Open Access Publishing Antarctic International Journal of Molecular Sciences 24 5 4850 |
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Open Polar |
collection |
MDPI Open Access Publishing |
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ftmdpi |
language |
English |
topic |
salivary microbiota stool microbiota metabolomics metabolite fingerprinting isolation longitudinal microbiota dynamics microbiota-metabolome integration |
spellingShingle |
salivary microbiota stool microbiota metabolomics metabolite fingerprinting isolation longitudinal microbiota dynamics microbiota-metabolome integration Simon J. S. Cameron Arwyn Edwards Robert J. Lambert Mike Stroud Luis A. J. Mur Participants in the Trans-Antarctic Winter Traverse Expedition Showed Increased Bacterial Load and Diversity in Saliva but Maintained Individual Differences within Stool Microbiota and Across Metabolite Fingerprints |
topic_facet |
salivary microbiota stool microbiota metabolomics metabolite fingerprinting isolation longitudinal microbiota dynamics microbiota-metabolome integration |
description |
Understanding the impact of long-term physiological and environmental stress on the human microbiota and metabolome may be important for the success of space flight. This work is logistically difficult and has a limited number of available participants. Terrestrial analogies present important opportunities to understand changes in the microbiota and metabolome and how this may impact participant health and fitness. Here, we present work from one such analogy: the Transarctic Winter Traverse expedition, which we believe is the first assessment of the microbiota and metabolome from different bodily locations during prolonged environmental and physiological stress. Bacterial load and diversity were significantly higher during the expedition when compared with baseline levels (p < 0.001) in saliva but not stool, and only a single operational taxonomic unit assigned to the Ruminococcaceae family shows significantly altered levels in stool (p < 0.001). Metabolite fingerprints show the maintenance of individual differences across saliva, stool, and plasma samples when analysed using flow infusion electrospray mass spectrometry and Fourier transform infrared spectroscopy. Significant activity-associated changes in terms of both bacterial diversity and load are seen in saliva but not in stool, and participant differences in metabolite fingerprints persist across all three sample types. |
format |
Text |
author |
Simon J. S. Cameron Arwyn Edwards Robert J. Lambert Mike Stroud Luis A. J. Mur |
author_facet |
Simon J. S. Cameron Arwyn Edwards Robert J. Lambert Mike Stroud Luis A. J. Mur |
author_sort |
Simon J. S. Cameron |
title |
Participants in the Trans-Antarctic Winter Traverse Expedition Showed Increased Bacterial Load and Diversity in Saliva but Maintained Individual Differences within Stool Microbiota and Across Metabolite Fingerprints |
title_short |
Participants in the Trans-Antarctic Winter Traverse Expedition Showed Increased Bacterial Load and Diversity in Saliva but Maintained Individual Differences within Stool Microbiota and Across Metabolite Fingerprints |
title_full |
Participants in the Trans-Antarctic Winter Traverse Expedition Showed Increased Bacterial Load and Diversity in Saliva but Maintained Individual Differences within Stool Microbiota and Across Metabolite Fingerprints |
title_fullStr |
Participants in the Trans-Antarctic Winter Traverse Expedition Showed Increased Bacterial Load and Diversity in Saliva but Maintained Individual Differences within Stool Microbiota and Across Metabolite Fingerprints |
title_full_unstemmed |
Participants in the Trans-Antarctic Winter Traverse Expedition Showed Increased Bacterial Load and Diversity in Saliva but Maintained Individual Differences within Stool Microbiota and Across Metabolite Fingerprints |
title_sort |
participants in the trans-antarctic winter traverse expedition showed increased bacterial load and diversity in saliva but maintained individual differences within stool microbiota and across metabolite fingerprints |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2023 |
url |
https://doi.org/10.3390/ijms24054850 |
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agris |
geographic |
Antarctic |
geographic_facet |
Antarctic |
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Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
International Journal of Molecular Sciences; Volume 24; Issue 5; Pages: 4850 |
op_relation |
Molecular Microbiology https://dx.doi.org/10.3390/ijms24054850 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/ijms24054850 |
container_title |
International Journal of Molecular Sciences |
container_volume |
24 |
container_issue |
5 |
container_start_page |
4850 |
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1774722488751620096 |