Molecular Changes in the Brain of the Wintering Calidris pusilla in the Mangroves of the Amazon River Estuary
Migrant birds prepare differently to fly north for breeding in the spring and for the flight to lower latitudes during autumn, avoiding the cold and food shortages of the Northern Hemisphere’s harsh winter. The molecular events associated with these fundamental stages in the life history of migrants...
Published in: | International Journal of Molecular Sciences |
---|---|
Main Authors: | , , , , , , , , , , , |
Format: | Text |
Language: | English |
Published: |
Multidisciplinary Digital Publishing Institute
2023
|
Subjects: | |
Online Access: | https://doi.org/10.3390/ijms241612712 |
id |
ftmdpi:oai:mdpi.com:/1422-0067/24/16/12712/ |
---|---|
record_format |
openpolar |
spelling |
ftmdpi:oai:mdpi.com:/1422-0067/24/16/12712/ 2023-09-05T13:17:45+02:00 Molecular Changes in the Brain of the Wintering Calidris pusilla in the Mangroves of the Amazon River Estuary Patrick Douglas Corrêa Pereira Ediely Pereira Henrique Emanuel Ramos da Costa Anderson de Jesus Falcão Mauro André Damasceno de Melo Maria Paula Cruz Schneider Rommel Mario Rodriguez Burbano Daniel Guerreiro Diniz Nara Gyzely de Morais Magalhães David Francis Sherry Cristovam Wanderley Picanço Diniz Cristovam Guerreiro-Diniz agris 2023-08-12 application/pdf https://doi.org/10.3390/ijms241612712 EN eng Multidisciplinary Digital Publishing Institute Molecular Neurobiology https://dx.doi.org/10.3390/ijms241612712 https://creativecommons.org/licenses/by/4.0/ International Journal of Molecular Sciences; Volume 24; Issue 16; Pages: 12712 brain transcriptome migratory birds spring migration wintering Calidris pusilla Text 2023 ftmdpi https://doi.org/10.3390/ijms241612712 2023-08-13T23:53:31Z Migrant birds prepare differently to fly north for breeding in the spring and for the flight to lower latitudes during autumn, avoiding the cold and food shortages of the Northern Hemisphere’s harsh winter. The molecular events associated with these fundamental stages in the life history of migrants include the differential gene expression in different tissues. Semipalmated sandpipers (Calidris pusilla) are Arctic-breeding shorebirds that migrate to the coast of South America during the non-breeding season. In a previous study, we demonstrated that between the beginning and the end of the wintering period, substantial glial changes and neurogenesis occur in the brain of C. pusilla. These changes follow the epic journey of the autumn migration when a 5-day non-stop transatlantic flight towards the coast of South America and the subsequent preparation for the long-distance flight of the spring migration takes place. Here, we tested the hypothesis that the differential gene expressions observed in the brains of individuals captured in the autumn and spring windows are consistent with the previously described cellular changes. We searched for differential gene expressions in the brain of the semipalmated sandpiper, of recently arrived birds (RA) from the autumnal migration, and that of individuals in the premigratory period (PM) in the spring. All individuals were collected in the tropical coastal of northern Brazil in the mangrove region of the Amazon River estuary. We generated a de novo neurotranscriptome for C. pusilla individuals and compared the gene expressions across libraries. To that end, we mapped an RNA-Seq that reads to the C. pusilla neurotranscriptome in four brain samples of each group and found that the differential gene expressions in newly arrived and premigratory birds were related with neurogenesis, metabolic pathways (ketone body biosynthetic and the catabolic and lipid biosynthetic processes), and glial changes (astrocyte-dopaminergic neuron signaling, astrocyte differentiation, astrocyte cell ... Text Arctic MDPI Open Access Publishing Arctic International Journal of Molecular Sciences 24 16 12712 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
brain transcriptome migratory birds spring migration wintering Calidris pusilla |
spellingShingle |
brain transcriptome migratory birds spring migration wintering Calidris pusilla Patrick Douglas Corrêa Pereira Ediely Pereira Henrique Emanuel Ramos da Costa Anderson de Jesus Falcão Mauro André Damasceno de Melo Maria Paula Cruz Schneider Rommel Mario Rodriguez Burbano Daniel Guerreiro Diniz Nara Gyzely de Morais Magalhães David Francis Sherry Cristovam Wanderley Picanço Diniz Cristovam Guerreiro-Diniz Molecular Changes in the Brain of the Wintering Calidris pusilla in the Mangroves of the Amazon River Estuary |
topic_facet |
brain transcriptome migratory birds spring migration wintering Calidris pusilla |
description |
Migrant birds prepare differently to fly north for breeding in the spring and for the flight to lower latitudes during autumn, avoiding the cold and food shortages of the Northern Hemisphere’s harsh winter. The molecular events associated with these fundamental stages in the life history of migrants include the differential gene expression in different tissues. Semipalmated sandpipers (Calidris pusilla) are Arctic-breeding shorebirds that migrate to the coast of South America during the non-breeding season. In a previous study, we demonstrated that between the beginning and the end of the wintering period, substantial glial changes and neurogenesis occur in the brain of C. pusilla. These changes follow the epic journey of the autumn migration when a 5-day non-stop transatlantic flight towards the coast of South America and the subsequent preparation for the long-distance flight of the spring migration takes place. Here, we tested the hypothesis that the differential gene expressions observed in the brains of individuals captured in the autumn and spring windows are consistent with the previously described cellular changes. We searched for differential gene expressions in the brain of the semipalmated sandpiper, of recently arrived birds (RA) from the autumnal migration, and that of individuals in the premigratory period (PM) in the spring. All individuals were collected in the tropical coastal of northern Brazil in the mangrove region of the Amazon River estuary. We generated a de novo neurotranscriptome for C. pusilla individuals and compared the gene expressions across libraries. To that end, we mapped an RNA-Seq that reads to the C. pusilla neurotranscriptome in four brain samples of each group and found that the differential gene expressions in newly arrived and premigratory birds were related with neurogenesis, metabolic pathways (ketone body biosynthetic and the catabolic and lipid biosynthetic processes), and glial changes (astrocyte-dopaminergic neuron signaling, astrocyte differentiation, astrocyte cell ... |
format |
Text |
author |
Patrick Douglas Corrêa Pereira Ediely Pereira Henrique Emanuel Ramos da Costa Anderson de Jesus Falcão Mauro André Damasceno de Melo Maria Paula Cruz Schneider Rommel Mario Rodriguez Burbano Daniel Guerreiro Diniz Nara Gyzely de Morais Magalhães David Francis Sherry Cristovam Wanderley Picanço Diniz Cristovam Guerreiro-Diniz |
author_facet |
Patrick Douglas Corrêa Pereira Ediely Pereira Henrique Emanuel Ramos da Costa Anderson de Jesus Falcão Mauro André Damasceno de Melo Maria Paula Cruz Schneider Rommel Mario Rodriguez Burbano Daniel Guerreiro Diniz Nara Gyzely de Morais Magalhães David Francis Sherry Cristovam Wanderley Picanço Diniz Cristovam Guerreiro-Diniz |
author_sort |
Patrick Douglas Corrêa Pereira |
title |
Molecular Changes in the Brain of the Wintering Calidris pusilla in the Mangroves of the Amazon River Estuary |
title_short |
Molecular Changes in the Brain of the Wintering Calidris pusilla in the Mangroves of the Amazon River Estuary |
title_full |
Molecular Changes in the Brain of the Wintering Calidris pusilla in the Mangroves of the Amazon River Estuary |
title_fullStr |
Molecular Changes in the Brain of the Wintering Calidris pusilla in the Mangroves of the Amazon River Estuary |
title_full_unstemmed |
Molecular Changes in the Brain of the Wintering Calidris pusilla in the Mangroves of the Amazon River Estuary |
title_sort |
molecular changes in the brain of the wintering calidris pusilla in the mangroves of the amazon river estuary |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2023 |
url |
https://doi.org/10.3390/ijms241612712 |
op_coverage |
agris |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
International Journal of Molecular Sciences; Volume 24; Issue 16; Pages: 12712 |
op_relation |
Molecular Neurobiology https://dx.doi.org/10.3390/ijms241612712 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/ijms241612712 |
container_title |
International Journal of Molecular Sciences |
container_volume |
24 |
container_issue |
16 |
container_start_page |
12712 |
_version_ |
1776198801663459328 |