Atlantic Salmon Mucins Inhibit LuxS-Dependent A. Salmonicida AI-2 Quorum Sensing in an N-Acetylneuraminic Acid-Dependent Manner
One of the most important bacterial diseases in salmonid aquaculture is furunculosis, caused by Aeromonas salmonicida. Bacterial communication through secreted autoinducer signals, quorum sensing, takes part in the regulation of gene expression in bacteria, influencing growth and virulence. The skin...
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ftmdpi:oai:mdpi.com:/1422-0067/23/8/4326/ 2023-08-20T04:05:13+02:00 Atlantic Salmon Mucins Inhibit LuxS-Dependent A. Salmonicida AI-2 Quorum Sensing in an N-Acetylneuraminic Acid-Dependent Manner János Tamás Padra Stefany Ojaimi Loibman Kaisa Thorell Henrik Sundh Kristina Sundell Sara K. Lindén agris 2022-04-13 application/pdf https://doi.org/10.3390/ijms23084326 EN eng Multidisciplinary Digital Publishing Institute Biochemistry https://dx.doi.org/10.3390/ijms23084326 https://creativecommons.org/licenses/by/4.0/ International Journal of Molecular Sciences; Volume 23; Issue 8; Pages: 4326 sialic acid signaling bacterial growth biofilm mucus Text 2022 ftmdpi https://doi.org/10.3390/ijms23084326 2023-08-01T04:45:16Z One of the most important bacterial diseases in salmonid aquaculture is furunculosis, caused by Aeromonas salmonicida. Bacterial communication through secreted autoinducer signals, quorum sensing, takes part in the regulation of gene expression in bacteria, influencing growth and virulence. The skin and mucosal surfaces, covered by a mucus layer, are the first point of contact between fish and bacteria. Mucins are highly glycosylated and are the main components of mucus. Here, we validate the Vibrio harveyi BB170 bioreporter assay for quantifying A. salmonicida quorum sensing and study the effects of Atlantic salmon mucins as well as mono- and disaccharides on the AI-2 levels of A. salmonicida. Atlantic salmon mucins from skin, pyloric ceca, proximal and distal intestine reduced A. salmonicida AI-2 levels. Among the saccharides abundant on mucins, fucose, N-acetylneuraminic acid and GlcNAcβ1-3Gal inhibited AI-2 A. salmonicida secretion. Removal of N-acetylneuraminic acid, which is the most abundant terminal residue on mucin glycans on Atlantic salmon mucins, attenuated the inhibitory effects on AI-2 levels of A. salmonicida. Deletion of A. salmonicidaluxS abolished AI-2 production. In conclusion, Atlantic salmon mucins regulate A. salmonicida quorum sensing in a luxS and N-acetylneuraminic acid-dependent manner. Text Atlantic salmon MDPI Open Access Publishing International Journal of Molecular Sciences 23 8 4326 |
institution |
Open Polar |
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MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
sialic acid signaling bacterial growth biofilm mucus |
spellingShingle |
sialic acid signaling bacterial growth biofilm mucus János Tamás Padra Stefany Ojaimi Loibman Kaisa Thorell Henrik Sundh Kristina Sundell Sara K. Lindén Atlantic Salmon Mucins Inhibit LuxS-Dependent A. Salmonicida AI-2 Quorum Sensing in an N-Acetylneuraminic Acid-Dependent Manner |
topic_facet |
sialic acid signaling bacterial growth biofilm mucus |
description |
One of the most important bacterial diseases in salmonid aquaculture is furunculosis, caused by Aeromonas salmonicida. Bacterial communication through secreted autoinducer signals, quorum sensing, takes part in the regulation of gene expression in bacteria, influencing growth and virulence. The skin and mucosal surfaces, covered by a mucus layer, are the first point of contact between fish and bacteria. Mucins are highly glycosylated and are the main components of mucus. Here, we validate the Vibrio harveyi BB170 bioreporter assay for quantifying A. salmonicida quorum sensing and study the effects of Atlantic salmon mucins as well as mono- and disaccharides on the AI-2 levels of A. salmonicida. Atlantic salmon mucins from skin, pyloric ceca, proximal and distal intestine reduced A. salmonicida AI-2 levels. Among the saccharides abundant on mucins, fucose, N-acetylneuraminic acid and GlcNAcβ1-3Gal inhibited AI-2 A. salmonicida secretion. Removal of N-acetylneuraminic acid, which is the most abundant terminal residue on mucin glycans on Atlantic salmon mucins, attenuated the inhibitory effects on AI-2 levels of A. salmonicida. Deletion of A. salmonicidaluxS abolished AI-2 production. In conclusion, Atlantic salmon mucins regulate A. salmonicida quorum sensing in a luxS and N-acetylneuraminic acid-dependent manner. |
format |
Text |
author |
János Tamás Padra Stefany Ojaimi Loibman Kaisa Thorell Henrik Sundh Kristina Sundell Sara K. Lindén |
author_facet |
János Tamás Padra Stefany Ojaimi Loibman Kaisa Thorell Henrik Sundh Kristina Sundell Sara K. Lindén |
author_sort |
János Tamás Padra |
title |
Atlantic Salmon Mucins Inhibit LuxS-Dependent A. Salmonicida AI-2 Quorum Sensing in an N-Acetylneuraminic Acid-Dependent Manner |
title_short |
Atlantic Salmon Mucins Inhibit LuxS-Dependent A. Salmonicida AI-2 Quorum Sensing in an N-Acetylneuraminic Acid-Dependent Manner |
title_full |
Atlantic Salmon Mucins Inhibit LuxS-Dependent A. Salmonicida AI-2 Quorum Sensing in an N-Acetylneuraminic Acid-Dependent Manner |
title_fullStr |
Atlantic Salmon Mucins Inhibit LuxS-Dependent A. Salmonicida AI-2 Quorum Sensing in an N-Acetylneuraminic Acid-Dependent Manner |
title_full_unstemmed |
Atlantic Salmon Mucins Inhibit LuxS-Dependent A. Salmonicida AI-2 Quorum Sensing in an N-Acetylneuraminic Acid-Dependent Manner |
title_sort |
atlantic salmon mucins inhibit luxs-dependent a. salmonicida ai-2 quorum sensing in an n-acetylneuraminic acid-dependent manner |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2022 |
url |
https://doi.org/10.3390/ijms23084326 |
op_coverage |
agris |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
International Journal of Molecular Sciences; Volume 23; Issue 8; Pages: 4326 |
op_relation |
Biochemistry https://dx.doi.org/10.3390/ijms23084326 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/ijms23084326 |
container_title |
International Journal of Molecular Sciences |
container_volume |
23 |
container_issue |
8 |
container_start_page |
4326 |
_version_ |
1774715705357238272 |