Genome-Wide DNA Methylation in Policemen Working in Cities Differing by Major Sources of Air Pollution
DNA methylation is the most studied epigenetic mechanism that regulates gene expression, and it can serve as a useful biomarker of prior environmental exposure and future health outcomes. This study focused on DNA methylation profiles in a human cohort, comprising 125 nonsmoking city policemen (samp...
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ftmdpi:oai:mdpi.com:/1422-0067/23/3/1666/ 2023-08-20T04:06:09+02:00 Genome-Wide DNA Methylation in Policemen Working in Cities Differing by Major Sources of Air Pollution Katerina Honkova Andrea Rossnerova Irena Chvojkova Alena Milcova Hasmik Margaryan Anna Pastorkova Antonin Ambroz Pavel Rossner Vitezslav Jirik Jiri Rubes Radim J. Sram Jan Topinka agris 2022-01-31 application/pdf https://doi.org/10.3390/ijms23031666 EN eng Multidisciplinary Digital Publishing Institute Molecular Toxicology https://dx.doi.org/10.3390/ijms23031666 https://creativecommons.org/licenses/by/4.0/ International Journal of Molecular Sciences; Volume 23; Issue 3; Pages: 1666 air pollution DNA methylation environment molecular epidemiology epigenetics Text 2022 ftmdpi https://doi.org/10.3390/ijms23031666 2023-08-01T04:01:54Z DNA methylation is the most studied epigenetic mechanism that regulates gene expression, and it can serve as a useful biomarker of prior environmental exposure and future health outcomes. This study focused on DNA methylation profiles in a human cohort, comprising 125 nonsmoking city policemen (sampled twice), living and working in three localities (Prague, Ostrava and Ceske Budejovice) of the Czech Republic, who spent the majority of their working time outdoors. The main characterization of the localities, differing by major sources of air pollution, was defined by the stationary air pollution monitoring of PM2.5, B[a]P and NO2. DNA methylation was analyzed by a genome-wide microarray method. No season-specific DNA methylation pattern was discovered; however, we identified 13,643 differentially methylated CpG loci (DML) for a comparison between the Prague and Ostrava groups. The most significant DML was cg10123377 (log2FC = −1.92, p = 8.30 × 10−4) and loci annotated to RPTOR (total 20 CpG loci). We also found two hypomethylated loci annotated to the DNA repair gene XRCC5. Groups of DML annotated to the same gene were linked to diabetes mellitus (KCNQ1), respiratory diseases (PTPRN2), the dopaminergic system of the brain and neurodegenerative diseases (NR4A2). The most significant possibly affected pathway was Axon guidance, with 86 potentially deregulated genes near DML. The cluster of gene sets that could be affected by DNA methylation in the Ostrava groups mainly includes the neuronal functions and biological processes of cell junctions and adhesion assembly. The study demonstrates that the differences in the type of air pollution between localities can affect a unique change in DNA methylation profiles across the human genome. Text DML MDPI Open Access Publishing International Journal of Molecular Sciences 23 3 1666 |
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MDPI Open Access Publishing |
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English |
topic |
air pollution DNA methylation environment molecular epidemiology epigenetics |
spellingShingle |
air pollution DNA methylation environment molecular epidemiology epigenetics Katerina Honkova Andrea Rossnerova Irena Chvojkova Alena Milcova Hasmik Margaryan Anna Pastorkova Antonin Ambroz Pavel Rossner Vitezslav Jirik Jiri Rubes Radim J. Sram Jan Topinka Genome-Wide DNA Methylation in Policemen Working in Cities Differing by Major Sources of Air Pollution |
topic_facet |
air pollution DNA methylation environment molecular epidemiology epigenetics |
description |
DNA methylation is the most studied epigenetic mechanism that regulates gene expression, and it can serve as a useful biomarker of prior environmental exposure and future health outcomes. This study focused on DNA methylation profiles in a human cohort, comprising 125 nonsmoking city policemen (sampled twice), living and working in three localities (Prague, Ostrava and Ceske Budejovice) of the Czech Republic, who spent the majority of their working time outdoors. The main characterization of the localities, differing by major sources of air pollution, was defined by the stationary air pollution monitoring of PM2.5, B[a]P and NO2. DNA methylation was analyzed by a genome-wide microarray method. No season-specific DNA methylation pattern was discovered; however, we identified 13,643 differentially methylated CpG loci (DML) for a comparison between the Prague and Ostrava groups. The most significant DML was cg10123377 (log2FC = −1.92, p = 8.30 × 10−4) and loci annotated to RPTOR (total 20 CpG loci). We also found two hypomethylated loci annotated to the DNA repair gene XRCC5. Groups of DML annotated to the same gene were linked to diabetes mellitus (KCNQ1), respiratory diseases (PTPRN2), the dopaminergic system of the brain and neurodegenerative diseases (NR4A2). The most significant possibly affected pathway was Axon guidance, with 86 potentially deregulated genes near DML. The cluster of gene sets that could be affected by DNA methylation in the Ostrava groups mainly includes the neuronal functions and biological processes of cell junctions and adhesion assembly. The study demonstrates that the differences in the type of air pollution between localities can affect a unique change in DNA methylation profiles across the human genome. |
format |
Text |
author |
Katerina Honkova Andrea Rossnerova Irena Chvojkova Alena Milcova Hasmik Margaryan Anna Pastorkova Antonin Ambroz Pavel Rossner Vitezslav Jirik Jiri Rubes Radim J. Sram Jan Topinka |
author_facet |
Katerina Honkova Andrea Rossnerova Irena Chvojkova Alena Milcova Hasmik Margaryan Anna Pastorkova Antonin Ambroz Pavel Rossner Vitezslav Jirik Jiri Rubes Radim J. Sram Jan Topinka |
author_sort |
Katerina Honkova |
title |
Genome-Wide DNA Methylation in Policemen Working in Cities Differing by Major Sources of Air Pollution |
title_short |
Genome-Wide DNA Methylation in Policemen Working in Cities Differing by Major Sources of Air Pollution |
title_full |
Genome-Wide DNA Methylation in Policemen Working in Cities Differing by Major Sources of Air Pollution |
title_fullStr |
Genome-Wide DNA Methylation in Policemen Working in Cities Differing by Major Sources of Air Pollution |
title_full_unstemmed |
Genome-Wide DNA Methylation in Policemen Working in Cities Differing by Major Sources of Air Pollution |
title_sort |
genome-wide dna methylation in policemen working in cities differing by major sources of air pollution |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2022 |
url |
https://doi.org/10.3390/ijms23031666 |
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agris |
genre |
DML |
genre_facet |
DML |
op_source |
International Journal of Molecular Sciences; Volume 23; Issue 3; Pages: 1666 |
op_relation |
Molecular Toxicology https://dx.doi.org/10.3390/ijms23031666 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/ijms23031666 |
container_title |
International Journal of Molecular Sciences |
container_volume |
23 |
container_issue |
3 |
container_start_page |
1666 |
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1774717078884843520 |