Proteomic Profiling Skin Mucus of European Eel Anguilla anguilla Infected with Anguillid Herpesvirus
Anguillid herpesvirus 1 (AngHV) is an important viral pathogen affecting eel. This study was designed to investigate the potential molecular mechanisms and immune response elicited at the protein levels in the skin mucus of AngHV-infected Anguilla anguilla. Tandem mass tag (TMT)-labelling proteomics...
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ftmdpi:oai:mdpi.com:/1422-0067/23/19/11283/ 2023-08-20T03:59:40+02:00 Proteomic Profiling Skin Mucus of European Eel Anguilla anguilla Infected with Anguillid Herpesvirus Ying-Ying Li Jin-Xian Yang Xi Chen Qiang Chen Tie-Ying Song Jun-Qing Ge agris 2022-09-24 application/pdf https://doi.org/10.3390/ijms231911283 EN eng Multidisciplinary Digital Publishing Institute Molecular Microbiology https://dx.doi.org/10.3390/ijms231911283 https://creativecommons.org/licenses/by/4.0/ International Journal of Molecular Sciences; Volume 23; Issue 19; Pages: 11283 Anguilla anguilla Anguillid herpesvirus TMT-labelled proteomic skin mucus Text 2022 ftmdpi https://doi.org/10.3390/ijms231911283 2023-08-01T06:36:57Z Anguillid herpesvirus 1 (AngHV) is an important viral pathogen affecting eel. This study was designed to investigate the potential molecular mechanisms and immune response elicited at the protein levels in the skin mucus of AngHV-infected Anguilla anguilla. Tandem mass tag (TMT)-labelling proteomics with the liquid chromatography tandem mass spectrometry (LC-MS/MS) was used for performing quantitative identification of the proteins. In addition, the quantitative protein amount was detected by parallel reaction monitoring (PRM) analysis. A total of 3486 proteins were identified, of which 2935 were quantified. When a protein fold change was greater than 1.3 or less than 0.76, it indicated a differentially expressed protein (DEP). Overall, 187 up-regulated proteins and 126 down-regulated proteins were detected, and most of the DEPs were enriched in the CAMs pathway, intestinal immune pathway, herpes simplex virus 1 infection pathway, phagosome pathway and p53 signaling pathway. The results of the DEPs detected by PRM were highly consistent with the results of the TMT-labelled quantitative proteomic analysis. The findings of this study provide an important research basis for further understanding the pathogenesis of AngHV. Text Anguilla anguilla MDPI Open Access Publishing International Journal of Molecular Sciences 23 19 11283 |
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MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
Anguilla anguilla Anguillid herpesvirus TMT-labelled proteomic skin mucus |
spellingShingle |
Anguilla anguilla Anguillid herpesvirus TMT-labelled proteomic skin mucus Ying-Ying Li Jin-Xian Yang Xi Chen Qiang Chen Tie-Ying Song Jun-Qing Ge Proteomic Profiling Skin Mucus of European Eel Anguilla anguilla Infected with Anguillid Herpesvirus |
topic_facet |
Anguilla anguilla Anguillid herpesvirus TMT-labelled proteomic skin mucus |
description |
Anguillid herpesvirus 1 (AngHV) is an important viral pathogen affecting eel. This study was designed to investigate the potential molecular mechanisms and immune response elicited at the protein levels in the skin mucus of AngHV-infected Anguilla anguilla. Tandem mass tag (TMT)-labelling proteomics with the liquid chromatography tandem mass spectrometry (LC-MS/MS) was used for performing quantitative identification of the proteins. In addition, the quantitative protein amount was detected by parallel reaction monitoring (PRM) analysis. A total of 3486 proteins were identified, of which 2935 were quantified. When a protein fold change was greater than 1.3 or less than 0.76, it indicated a differentially expressed protein (DEP). Overall, 187 up-regulated proteins and 126 down-regulated proteins were detected, and most of the DEPs were enriched in the CAMs pathway, intestinal immune pathway, herpes simplex virus 1 infection pathway, phagosome pathway and p53 signaling pathway. The results of the DEPs detected by PRM were highly consistent with the results of the TMT-labelled quantitative proteomic analysis. The findings of this study provide an important research basis for further understanding the pathogenesis of AngHV. |
format |
Text |
author |
Ying-Ying Li Jin-Xian Yang Xi Chen Qiang Chen Tie-Ying Song Jun-Qing Ge |
author_facet |
Ying-Ying Li Jin-Xian Yang Xi Chen Qiang Chen Tie-Ying Song Jun-Qing Ge |
author_sort |
Ying-Ying Li |
title |
Proteomic Profiling Skin Mucus of European Eel Anguilla anguilla Infected with Anguillid Herpesvirus |
title_short |
Proteomic Profiling Skin Mucus of European Eel Anguilla anguilla Infected with Anguillid Herpesvirus |
title_full |
Proteomic Profiling Skin Mucus of European Eel Anguilla anguilla Infected with Anguillid Herpesvirus |
title_fullStr |
Proteomic Profiling Skin Mucus of European Eel Anguilla anguilla Infected with Anguillid Herpesvirus |
title_full_unstemmed |
Proteomic Profiling Skin Mucus of European Eel Anguilla anguilla Infected with Anguillid Herpesvirus |
title_sort |
proteomic profiling skin mucus of european eel anguilla anguilla infected with anguillid herpesvirus |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2022 |
url |
https://doi.org/10.3390/ijms231911283 |
op_coverage |
agris |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_source |
International Journal of Molecular Sciences; Volume 23; Issue 19; Pages: 11283 |
op_relation |
Molecular Microbiology https://dx.doi.org/10.3390/ijms231911283 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/ijms231911283 |
container_title |
International Journal of Molecular Sciences |
container_volume |
23 |
container_issue |
19 |
container_start_page |
11283 |
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1774714370015625216 |