Identification and Characterization of the Larval Settlement Pheromone Protein Components in Adult Shells of Crassostrea gigas: A Novel Function of Shell Matrix Proteins
The global decline of natural oyster populations emphasizes the need to improve our understanding of their biology. Understanding the role of chemical cues from conspecifics on how oysters occupy appropriate substrata is crucial to learning about their evolution, population dynamics, and chemical co...
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ftmdpi:oai:mdpi.com:/1422-0067/23/17/9816/ 2023-08-20T04:06:03+02:00 Identification and Characterization of the Larval Settlement Pheromone Protein Components in Adult Shells of Crassostrea gigas: A Novel Function of Shell Matrix Proteins Mary Grace Sedanza Asami Yoshida Hee-Jin Kim Kenichi Yamaguchi Kiyoshi Osatomi Cyril Glenn Satuito agris 2022-08-29 application/pdf https://doi.org/10.3390/ijms23179816 EN eng Multidisciplinary Digital Publishing Institute Molecular Biology https://dx.doi.org/10.3390/ijms23179816 https://creativecommons.org/licenses/by/4.0/ International Journal of Molecular Sciences; Volume 23; Issue 17; Pages: 9816 oyster larval ecology conspecific cues settlement induction marine chemical ecology biomineralization post-translational modifications lectin-glycan interaction Text 2022 ftmdpi https://doi.org/10.3390/ijms23179816 2023-08-01T06:16:01Z The global decline of natural oyster populations emphasizes the need to improve our understanding of their biology. Understanding the role of chemical cues from conspecifics on how oysters occupy appropriate substrata is crucial to learning about their evolution, population dynamics, and chemical communication. Here, a novel role of a macromolecular assembly of shell matrix proteins which act as Crassostrea gigas Settlement Pheromone Protein Components in adult shells is demonstrated as the biological cue responsible for gregarious settlement on conspecifics. A bioassay-guided fractionation approach aided by biochemical and molecular analyses reveals that Gigasin-6 isoform X1 and/or X2 isolated from adult shells is the major inducing cue for larval settlement and may also play a role in postlarva–larva settlement interactions. Other isolated Stains-all-stainable acidic proteins may function as a co-factor and a scaffold/structural framework for other matrix proteins to anchor within this assembly and provide protection. Notably, conspecific cue-mediated larval settlement induction in C. gigas presents a complex system that requires an interplay of different glycans, disulfide bonds, amino acid groups, and phosphorylation crosstalk for recognition. These results may find application in the development of oyster aquacultures which could help recover declining marine species and as targets of anti-fouling agents. Text Crassostrea gigas MDPI Open Access Publishing International Journal of Molecular Sciences 23 17 9816 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
oyster larval ecology conspecific cues settlement induction marine chemical ecology biomineralization post-translational modifications lectin-glycan interaction |
spellingShingle |
oyster larval ecology conspecific cues settlement induction marine chemical ecology biomineralization post-translational modifications lectin-glycan interaction Mary Grace Sedanza Asami Yoshida Hee-Jin Kim Kenichi Yamaguchi Kiyoshi Osatomi Cyril Glenn Satuito Identification and Characterization of the Larval Settlement Pheromone Protein Components in Adult Shells of Crassostrea gigas: A Novel Function of Shell Matrix Proteins |
topic_facet |
oyster larval ecology conspecific cues settlement induction marine chemical ecology biomineralization post-translational modifications lectin-glycan interaction |
description |
The global decline of natural oyster populations emphasizes the need to improve our understanding of their biology. Understanding the role of chemical cues from conspecifics on how oysters occupy appropriate substrata is crucial to learning about their evolution, population dynamics, and chemical communication. Here, a novel role of a macromolecular assembly of shell matrix proteins which act as Crassostrea gigas Settlement Pheromone Protein Components in adult shells is demonstrated as the biological cue responsible for gregarious settlement on conspecifics. A bioassay-guided fractionation approach aided by biochemical and molecular analyses reveals that Gigasin-6 isoform X1 and/or X2 isolated from adult shells is the major inducing cue for larval settlement and may also play a role in postlarva–larva settlement interactions. Other isolated Stains-all-stainable acidic proteins may function as a co-factor and a scaffold/structural framework for other matrix proteins to anchor within this assembly and provide protection. Notably, conspecific cue-mediated larval settlement induction in C. gigas presents a complex system that requires an interplay of different glycans, disulfide bonds, amino acid groups, and phosphorylation crosstalk for recognition. These results may find application in the development of oyster aquacultures which could help recover declining marine species and as targets of anti-fouling agents. |
format |
Text |
author |
Mary Grace Sedanza Asami Yoshida Hee-Jin Kim Kenichi Yamaguchi Kiyoshi Osatomi Cyril Glenn Satuito |
author_facet |
Mary Grace Sedanza Asami Yoshida Hee-Jin Kim Kenichi Yamaguchi Kiyoshi Osatomi Cyril Glenn Satuito |
author_sort |
Mary Grace Sedanza |
title |
Identification and Characterization of the Larval Settlement Pheromone Protein Components in Adult Shells of Crassostrea gigas: A Novel Function of Shell Matrix Proteins |
title_short |
Identification and Characterization of the Larval Settlement Pheromone Protein Components in Adult Shells of Crassostrea gigas: A Novel Function of Shell Matrix Proteins |
title_full |
Identification and Characterization of the Larval Settlement Pheromone Protein Components in Adult Shells of Crassostrea gigas: A Novel Function of Shell Matrix Proteins |
title_fullStr |
Identification and Characterization of the Larval Settlement Pheromone Protein Components in Adult Shells of Crassostrea gigas: A Novel Function of Shell Matrix Proteins |
title_full_unstemmed |
Identification and Characterization of the Larval Settlement Pheromone Protein Components in Adult Shells of Crassostrea gigas: A Novel Function of Shell Matrix Proteins |
title_sort |
identification and characterization of the larval settlement pheromone protein components in adult shells of crassostrea gigas: a novel function of shell matrix proteins |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2022 |
url |
https://doi.org/10.3390/ijms23179816 |
op_coverage |
agris |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
International Journal of Molecular Sciences; Volume 23; Issue 17; Pages: 9816 |
op_relation |
Molecular Biology https://dx.doi.org/10.3390/ijms23179816 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/ijms23179816 |
container_title |
International Journal of Molecular Sciences |
container_volume |
23 |
container_issue |
17 |
container_start_page |
9816 |
_version_ |
1774716954851934208 |