Exopolysaccharide ID1 Improves Post-Warming Outcomes after Vitrification of In Vitro-Produced Bovine Embryos
This study aimed to assess the cryoprotectant role of exopolysaccharide (EPS) ID1, produced by Antarctic Pseudomonas sp., in the vitrification of in vitro-produced (IVP) bovine embryos. IVP day 7 (D7) and day 8 (D8) expanded blastocysts derived from cow or calf oocytes were vitrified without supplem...
Published in: | International Journal of Molecular Sciences |
---|---|
Main Authors: | , , , , , , |
Format: | Text |
Language: | English |
Published: |
Multidisciplinary Digital Publishing Institute
2022
|
Subjects: | |
Online Access: | https://doi.org/10.3390/ijms23137069 |
id |
ftmdpi:oai:mdpi.com:/1422-0067/23/13/7069/ |
---|---|
record_format |
openpolar |
spelling |
ftmdpi:oai:mdpi.com:/1422-0067/23/13/7069/ 2023-08-20T04:02:12+02:00 Exopolysaccharide ID1 Improves Post-Warming Outcomes after Vitrification of In Vitro-Produced Bovine Embryos Erika Alina Ordóñez-León Iris Martínez-Rodero Tania García-Martínez Manel López-Béjar Marc Yeste Elena Mercade Teresa Mogas agris 2022-06-25 application/pdf https://doi.org/10.3390/ijms23137069 EN eng Multidisciplinary Digital Publishing Institute Molecular Biology https://dx.doi.org/10.3390/ijms23137069 https://creativecommons.org/licenses/by/4.0/ International Journal of Molecular Sciences; Volume 23; Issue 13; Pages: 7069 cryopreservation blastocyst total cell number inner cell mass TUNEL embryo development gene expression regulation Text 2022 ftmdpi https://doi.org/10.3390/ijms23137069 2023-08-01T05:29:48Z This study aimed to assess the cryoprotectant role of exopolysaccharide (EPS) ID1, produced by Antarctic Pseudomonas sp., in the vitrification of in vitro-produced (IVP) bovine embryos. IVP day 7 (D7) and day 8 (D8) expanded blastocysts derived from cow or calf oocytes were vitrified without supplementation (EPS0) or supplemented with 10 µg/mL (EPS10) or 100 µg/mL (EPS100) EPS ID1. The effect of EPS ID1 was assessed in post-warming re-expansion and hatching rates, differential cell count, apoptosis rate, and gene expression. EPS100 re-expansion rates were significantly higher than those observed for the EPS0 and EPS10 treatments, regardless of culture length or oocyte source. EPS100 hatching rate was similar to the one of the fresh blastocysts except for those D7 blastocysts derived from calf oocytes. No differences were observed among EPS ID1 treatments when the inner cell mass, trophectoderm, and total cell number were assessed. Although apoptosis rates were higher (p ≤ 0.05) in vitrified groups compared to fresh embryos, EPS100 blastocysts had a lower number (p ≤ 0.05) of apoptotic nuclei than the EPS0 or EPS10 groups. No differences in the expression of BCL2, AQP3, CX43, and SOD1 genes between treatments were observed. Vitrification without EPS ID1 supplementation produced blastocysts with significantly higher BAX gene expression, whereas treatment with 100 µg/mL EPS ID1 returned BAX levels to those observed in non-vitrified blastocysts. Our results suggest that 100 µg/mL EPS ID1 added to the vitrification media is beneficial for embryo cryopreservation because it results in higher re-expansion and hatching ability and it positively modulates apoptosis. Text Antarc* Antarctic MDPI Open Access Publishing Antarctic International Journal of Molecular Sciences 23 13 7069 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
cryopreservation blastocyst total cell number inner cell mass TUNEL embryo development gene expression regulation |
spellingShingle |
cryopreservation blastocyst total cell number inner cell mass TUNEL embryo development gene expression regulation Erika Alina Ordóñez-León Iris Martínez-Rodero Tania García-Martínez Manel López-Béjar Marc Yeste Elena Mercade Teresa Mogas Exopolysaccharide ID1 Improves Post-Warming Outcomes after Vitrification of In Vitro-Produced Bovine Embryos |
topic_facet |
cryopreservation blastocyst total cell number inner cell mass TUNEL embryo development gene expression regulation |
description |
This study aimed to assess the cryoprotectant role of exopolysaccharide (EPS) ID1, produced by Antarctic Pseudomonas sp., in the vitrification of in vitro-produced (IVP) bovine embryos. IVP day 7 (D7) and day 8 (D8) expanded blastocysts derived from cow or calf oocytes were vitrified without supplementation (EPS0) or supplemented with 10 µg/mL (EPS10) or 100 µg/mL (EPS100) EPS ID1. The effect of EPS ID1 was assessed in post-warming re-expansion and hatching rates, differential cell count, apoptosis rate, and gene expression. EPS100 re-expansion rates were significantly higher than those observed for the EPS0 and EPS10 treatments, regardless of culture length or oocyte source. EPS100 hatching rate was similar to the one of the fresh blastocysts except for those D7 blastocysts derived from calf oocytes. No differences were observed among EPS ID1 treatments when the inner cell mass, trophectoderm, and total cell number were assessed. Although apoptosis rates were higher (p ≤ 0.05) in vitrified groups compared to fresh embryos, EPS100 blastocysts had a lower number (p ≤ 0.05) of apoptotic nuclei than the EPS0 or EPS10 groups. No differences in the expression of BCL2, AQP3, CX43, and SOD1 genes between treatments were observed. Vitrification without EPS ID1 supplementation produced blastocysts with significantly higher BAX gene expression, whereas treatment with 100 µg/mL EPS ID1 returned BAX levels to those observed in non-vitrified blastocysts. Our results suggest that 100 µg/mL EPS ID1 added to the vitrification media is beneficial for embryo cryopreservation because it results in higher re-expansion and hatching ability and it positively modulates apoptosis. |
format |
Text |
author |
Erika Alina Ordóñez-León Iris Martínez-Rodero Tania García-Martínez Manel López-Béjar Marc Yeste Elena Mercade Teresa Mogas |
author_facet |
Erika Alina Ordóñez-León Iris Martínez-Rodero Tania García-Martínez Manel López-Béjar Marc Yeste Elena Mercade Teresa Mogas |
author_sort |
Erika Alina Ordóñez-León |
title |
Exopolysaccharide ID1 Improves Post-Warming Outcomes after Vitrification of In Vitro-Produced Bovine Embryos |
title_short |
Exopolysaccharide ID1 Improves Post-Warming Outcomes after Vitrification of In Vitro-Produced Bovine Embryos |
title_full |
Exopolysaccharide ID1 Improves Post-Warming Outcomes after Vitrification of In Vitro-Produced Bovine Embryos |
title_fullStr |
Exopolysaccharide ID1 Improves Post-Warming Outcomes after Vitrification of In Vitro-Produced Bovine Embryos |
title_full_unstemmed |
Exopolysaccharide ID1 Improves Post-Warming Outcomes after Vitrification of In Vitro-Produced Bovine Embryos |
title_sort |
exopolysaccharide id1 improves post-warming outcomes after vitrification of in vitro-produced bovine embryos |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2022 |
url |
https://doi.org/10.3390/ijms23137069 |
op_coverage |
agris |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
International Journal of Molecular Sciences; Volume 23; Issue 13; Pages: 7069 |
op_relation |
Molecular Biology https://dx.doi.org/10.3390/ijms23137069 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/ijms23137069 |
container_title |
International Journal of Molecular Sciences |
container_volume |
23 |
container_issue |
13 |
container_start_page |
7069 |
_version_ |
1774712573758799872 |