Integration of Maps Enables a Cytogenomics Analysis of the Complete Karyotype in Solea senegalensis
The Pleuronectiformes order, which includes several commercially-important species, has undergone extensive chromosome evolution. One of these species is Solea senegalensis, a flatfish with 2n = 42 chromosomes. In this study, a cytogenomics approach and integration with previous maps was applied to...
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ftmdpi:oai:mdpi.com:/1422-0067/23/10/5353/ 2023-08-20T04:09:40+02:00 Integration of Maps Enables a Cytogenomics Analysis of the Complete Karyotype in Solea senegalensis Daniel Ramírez María Esther Rodríguez Ismael Cross Alberto Arias-Pérez Manuel Alejandro Merlo Marco Anaya Silvia Portela-Bens Paulino Martínez Francisca Robles Carmelo Ruiz-Rejón Laureana Rebordinos agris 2022-05-11 application/pdf https://doi.org/10.3390/ijms23105353 EN eng Multidisciplinary Digital Publishing Institute Molecular Biology https://dx.doi.org/10.3390/ijms23105353 https://creativecommons.org/licenses/by/4.0/ International Journal of Molecular Sciences; Volume 23; Issue 10; Pages: 5353 Solea senegalensis pleuronectiformes genetic maps cytogenomics chromosome evolution karyotype repetitive sequences comparative genomics Text 2022 ftmdpi https://doi.org/10.3390/ijms23105353 2023-08-01T05:01:07Z The Pleuronectiformes order, which includes several commercially-important species, has undergone extensive chromosome evolution. One of these species is Solea senegalensis, a flatfish with 2n = 42 chromosomes. In this study, a cytogenomics approach and integration with previous maps was applied to characterize the karyotype of the species. Synteny analysis of S. senegalensis was carried out using two flatfish as a reference: Cynoglossus semilaevis and Scophthalmus maximus. Most S. senegalensis chromosomes (or chromosome arms for metacentrics and submetacentrics) showed a one-to-one macrosyntenic pattern with the other two species. In addition, we studied how repetitive sequences could have played a role in the evolution of S. senegalensis bi-armed (3, and 5–9) and acrocentric (11, 12 and 16) chromosomes, which showed the highest rearrangements compared with the reference species. A higher abundance of TEs (Transposable Elements) and other repeated elements was observed adjacent to telomeric regions on chromosomes 3, 7, 9 and 16. However, on chromosome 11, a greater abundance of DNA transposons was detected in interstitial BACs. This chromosome is syntenic with several chromosomes of the other two flatfish species, suggesting rearrangements during its evolution. A similar situation was also found on chromosome 16 (for microsatellites and low complexity sequences), but not for TEs (retroelements and DNA transposons). These differences in the distribution and abundance of repetitive elements in chromosomes that have undergone remodeling processes during the course of evolution also suggest a possible role for simple repeat sequences in rearranged regions. Text Scophthalmus maximus MDPI Open Access Publishing International Journal of Molecular Sciences 23 10 5353 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
Solea senegalensis pleuronectiformes genetic maps cytogenomics chromosome evolution karyotype repetitive sequences comparative genomics |
spellingShingle |
Solea senegalensis pleuronectiformes genetic maps cytogenomics chromosome evolution karyotype repetitive sequences comparative genomics Daniel Ramírez María Esther Rodríguez Ismael Cross Alberto Arias-Pérez Manuel Alejandro Merlo Marco Anaya Silvia Portela-Bens Paulino Martínez Francisca Robles Carmelo Ruiz-Rejón Laureana Rebordinos Integration of Maps Enables a Cytogenomics Analysis of the Complete Karyotype in Solea senegalensis |
topic_facet |
Solea senegalensis pleuronectiformes genetic maps cytogenomics chromosome evolution karyotype repetitive sequences comparative genomics |
description |
The Pleuronectiformes order, which includes several commercially-important species, has undergone extensive chromosome evolution. One of these species is Solea senegalensis, a flatfish with 2n = 42 chromosomes. In this study, a cytogenomics approach and integration with previous maps was applied to characterize the karyotype of the species. Synteny analysis of S. senegalensis was carried out using two flatfish as a reference: Cynoglossus semilaevis and Scophthalmus maximus. Most S. senegalensis chromosomes (or chromosome arms for metacentrics and submetacentrics) showed a one-to-one macrosyntenic pattern with the other two species. In addition, we studied how repetitive sequences could have played a role in the evolution of S. senegalensis bi-armed (3, and 5–9) and acrocentric (11, 12 and 16) chromosomes, which showed the highest rearrangements compared with the reference species. A higher abundance of TEs (Transposable Elements) and other repeated elements was observed adjacent to telomeric regions on chromosomes 3, 7, 9 and 16. However, on chromosome 11, a greater abundance of DNA transposons was detected in interstitial BACs. This chromosome is syntenic with several chromosomes of the other two flatfish species, suggesting rearrangements during its evolution. A similar situation was also found on chromosome 16 (for microsatellites and low complexity sequences), but not for TEs (retroelements and DNA transposons). These differences in the distribution and abundance of repetitive elements in chromosomes that have undergone remodeling processes during the course of evolution also suggest a possible role for simple repeat sequences in rearranged regions. |
format |
Text |
author |
Daniel Ramírez María Esther Rodríguez Ismael Cross Alberto Arias-Pérez Manuel Alejandro Merlo Marco Anaya Silvia Portela-Bens Paulino Martínez Francisca Robles Carmelo Ruiz-Rejón Laureana Rebordinos |
author_facet |
Daniel Ramírez María Esther Rodríguez Ismael Cross Alberto Arias-Pérez Manuel Alejandro Merlo Marco Anaya Silvia Portela-Bens Paulino Martínez Francisca Robles Carmelo Ruiz-Rejón Laureana Rebordinos |
author_sort |
Daniel Ramírez |
title |
Integration of Maps Enables a Cytogenomics Analysis of the Complete Karyotype in Solea senegalensis |
title_short |
Integration of Maps Enables a Cytogenomics Analysis of the Complete Karyotype in Solea senegalensis |
title_full |
Integration of Maps Enables a Cytogenomics Analysis of the Complete Karyotype in Solea senegalensis |
title_fullStr |
Integration of Maps Enables a Cytogenomics Analysis of the Complete Karyotype in Solea senegalensis |
title_full_unstemmed |
Integration of Maps Enables a Cytogenomics Analysis of the Complete Karyotype in Solea senegalensis |
title_sort |
integration of maps enables a cytogenomics analysis of the complete karyotype in solea senegalensis |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2022 |
url |
https://doi.org/10.3390/ijms23105353 |
op_coverage |
agris |
genre |
Scophthalmus maximus |
genre_facet |
Scophthalmus maximus |
op_source |
International Journal of Molecular Sciences; Volume 23; Issue 10; Pages: 5353 |
op_relation |
Molecular Biology https://dx.doi.org/10.3390/ijms23105353 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/ijms23105353 |
container_title |
International Journal of Molecular Sciences |
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23 |
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10 |
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5353 |
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1774723275852611584 |