Transient Receptor Potential (TRP) Channels in the Pacific Oyster (Crassostrea gigas): Genome-Wide Identification and Expression Profiling after Heat Stress between C. gigas and C. angulata
Transmembrane proteins are involved in an array of stress responses, particularly in thermo-sensation and thermo-regulation. In this study, we performed a genome-wide identification and characterization of the Transient Receptor Potential (TRP) genes in the Pacific oyster (Crassostrea gigas) and inv...
Published in: | International Journal of Molecular Sciences |
---|---|
Main Authors: | , , , , , , , |
Format: | Text |
Language: | English |
Published: |
Multidisciplinary Digital Publishing Institute
2021
|
Subjects: | |
Online Access: | https://doi.org/10.3390/ijms22063222 |
id |
ftmdpi:oai:mdpi.com:/1422-0067/22/6/3222/ |
---|---|
record_format |
openpolar |
spelling |
ftmdpi:oai:mdpi.com:/1422-0067/22/6/3222/ 2023-08-20T04:06:01+02:00 Transient Receptor Potential (TRP) Channels in the Pacific Oyster (Crassostrea gigas): Genome-Wide Identification and Expression Profiling after Heat Stress between C. gigas and C. angulata Huiru Fu Zexin Jiao Yongjing Li Jing Tian Liting Ren Fuqiang Zhang Qi Li Shikai Liu agris 2021-03-22 application/pdf https://doi.org/10.3390/ijms22063222 EN eng Multidisciplinary Digital Publishing Institute Biochemistry https://dx.doi.org/10.3390/ijms22063222 https://creativecommons.org/licenses/by/4.0/ International Journal of Molecular Sciences; Volume 22; Issue 6; Pages: 3222 Crassostrea gigas TRPs phylogeny gene expression thermal stress Text 2021 ftmdpi https://doi.org/10.3390/ijms22063222 2023-08-01T01:20:30Z Transmembrane proteins are involved in an array of stress responses, particularly in thermo-sensation and thermo-regulation. In this study, we performed a genome-wide identification and characterization of the Transient Receptor Potential (TRP) genes in the Pacific oyster (Crassostrea gigas) and investigated their expression profiles after heat stress to identify critical TRPs potentially associated with thermal regulation. A total of 66 TRP genes were identified in the C. gigas, which showed significant gene expansion and tandem duplication. Meta-analysis of the available RNA-Seq data generated from samples after acute heat stress revealed a set of heat-inducible TRPs. Further examination of their expression profiles under chronic heat stress, and comparison between C. gigas and C. angulata, two oyster species with different tolerance levels to heat stress, led to the identification of TRPC3.6, TRPC3.7, and TRPV4.7 as important TRPs involved in thermal regulation in oysters. This work provided valuable information for future studies on the molecular mechanism of TRP mediated thermal tolerance, and identification of diagnostic biomarker for thermal stress in the oysters. Text Crassostrea gigas Pacific oyster MDPI Open Access Publishing Pacific International Journal of Molecular Sciences 22 6 3222 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
Crassostrea gigas TRPs phylogeny gene expression thermal stress |
spellingShingle |
Crassostrea gigas TRPs phylogeny gene expression thermal stress Huiru Fu Zexin Jiao Yongjing Li Jing Tian Liting Ren Fuqiang Zhang Qi Li Shikai Liu Transient Receptor Potential (TRP) Channels in the Pacific Oyster (Crassostrea gigas): Genome-Wide Identification and Expression Profiling after Heat Stress between C. gigas and C. angulata |
topic_facet |
Crassostrea gigas TRPs phylogeny gene expression thermal stress |
description |
Transmembrane proteins are involved in an array of stress responses, particularly in thermo-sensation and thermo-regulation. In this study, we performed a genome-wide identification and characterization of the Transient Receptor Potential (TRP) genes in the Pacific oyster (Crassostrea gigas) and investigated their expression profiles after heat stress to identify critical TRPs potentially associated with thermal regulation. A total of 66 TRP genes were identified in the C. gigas, which showed significant gene expansion and tandem duplication. Meta-analysis of the available RNA-Seq data generated from samples after acute heat stress revealed a set of heat-inducible TRPs. Further examination of their expression profiles under chronic heat stress, and comparison between C. gigas and C. angulata, two oyster species with different tolerance levels to heat stress, led to the identification of TRPC3.6, TRPC3.7, and TRPV4.7 as important TRPs involved in thermal regulation in oysters. This work provided valuable information for future studies on the molecular mechanism of TRP mediated thermal tolerance, and identification of diagnostic biomarker for thermal stress in the oysters. |
format |
Text |
author |
Huiru Fu Zexin Jiao Yongjing Li Jing Tian Liting Ren Fuqiang Zhang Qi Li Shikai Liu |
author_facet |
Huiru Fu Zexin Jiao Yongjing Li Jing Tian Liting Ren Fuqiang Zhang Qi Li Shikai Liu |
author_sort |
Huiru Fu |
title |
Transient Receptor Potential (TRP) Channels in the Pacific Oyster (Crassostrea gigas): Genome-Wide Identification and Expression Profiling after Heat Stress between C. gigas and C. angulata |
title_short |
Transient Receptor Potential (TRP) Channels in the Pacific Oyster (Crassostrea gigas): Genome-Wide Identification and Expression Profiling after Heat Stress between C. gigas and C. angulata |
title_full |
Transient Receptor Potential (TRP) Channels in the Pacific Oyster (Crassostrea gigas): Genome-Wide Identification and Expression Profiling after Heat Stress between C. gigas and C. angulata |
title_fullStr |
Transient Receptor Potential (TRP) Channels in the Pacific Oyster (Crassostrea gigas): Genome-Wide Identification and Expression Profiling after Heat Stress between C. gigas and C. angulata |
title_full_unstemmed |
Transient Receptor Potential (TRP) Channels in the Pacific Oyster (Crassostrea gigas): Genome-Wide Identification and Expression Profiling after Heat Stress between C. gigas and C. angulata |
title_sort |
transient receptor potential (trp) channels in the pacific oyster (crassostrea gigas): genome-wide identification and expression profiling after heat stress between c. gigas and c. angulata |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2021 |
url |
https://doi.org/10.3390/ijms22063222 |
op_coverage |
agris |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
International Journal of Molecular Sciences; Volume 22; Issue 6; Pages: 3222 |
op_relation |
Biochemistry https://dx.doi.org/10.3390/ijms22063222 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/ijms22063222 |
container_title |
International Journal of Molecular Sciences |
container_volume |
22 |
container_issue |
6 |
container_start_page |
3222 |
_version_ |
1774716917829861376 |