The Simple Method of Preparation of Highly Carboxylated Bacterial Cellulose with Ni- and Mg-Ferrite-Based Versatile Magnetic Carrier for Enzyme Immobilization
The bacterial cellulose (BC) is a versatile biopolymer of microbial origin characterized by high purity and unusual water and material properties. However, the native BC contains a low number of functional groups, which significantly limits its further application. The main goal of its effective mod...
Published in: | International Journal of Molecular Sciences |
---|---|
Main Authors: | , , , , , |
Format: | Text |
Language: | English |
Published: |
Multidisciplinary Digital Publishing Institute
2021
|
Subjects: | |
Online Access: | https://doi.org/10.3390/ijms22168563 |
id |
ftmdpi:oai:mdpi.com:/1422-0067/22/16/8563/ |
---|---|
record_format |
openpolar |
spelling |
ftmdpi:oai:mdpi.com:/1422-0067/22/16/8563/ 2023-08-20T04:02:22+02:00 The Simple Method of Preparation of Highly Carboxylated Bacterial Cellulose with Ni- and Mg-Ferrite-Based Versatile Magnetic Carrier for Enzyme Immobilization Radosław Drozd Magdalena Szymańska Katarzyna Przygrodzka Jakub Hoppe Grzegorz Leniec Urszula Kowalska agris 2021-08-09 application/pdf https://doi.org/10.3390/ijms22168563 EN eng Multidisciplinary Digital Publishing Institute Macromolecules https://dx.doi.org/10.3390/ijms22168563 https://creativecommons.org/licenses/by/4.0/ International Journal of Molecular Sciences; Volume 22; Issue 16; Pages: 8563 bacterial cellulose magnetic carrier carboxylation citric acid immobilization Text 2021 ftmdpi https://doi.org/10.3390/ijms22168563 2023-08-01T02:23:43Z The bacterial cellulose (BC) is a versatile biopolymer of microbial origin characterized by high purity and unusual water and material properties. However, the native BC contains a low number of functional groups, which significantly limits its further application. The main goal of its effective modification is to use methods that allow the unusual properties of BC to be retained and the desired functional group to be efficiently introduced. In the present study, the new magnetic carrier based on functionalized citric acid (CA) bacterial cellulose was developed and tested to support critical industrial enzymes such as lipase B from Candida antarctica and phospholipase A from Aspergillus oryzae. The applied method allowed BC to be effectively modified by citric acid and a sufficient number of carboxylic groups to be introduced, up to 3.6 mmol of COOH per gram of dry mass of the prepared carrier. The DSC and TGA analyses revealed carrier stability at operational temperatures in the range of 20 °C to 100 °C and substantially influenced the amount of the introduced carboxyl groups on carrier properties. Both enzymes’ immobilization significantly improves their thermal stability at 60 °C without a significant thermal and pH optima effect. The analyzed enzymes showed good operational stability with a significant residual activity after ten cycles of repeated uses. The new magnetic carrier based on highly carboxylated bacterial cellulose has a high application capability as matrix for immobilization the various enzymes of industrial interest. Text Antarc* Antarctica MDPI Open Access Publishing International Journal of Molecular Sciences 22 16 8563 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
bacterial cellulose magnetic carrier carboxylation citric acid immobilization |
spellingShingle |
bacterial cellulose magnetic carrier carboxylation citric acid immobilization Radosław Drozd Magdalena Szymańska Katarzyna Przygrodzka Jakub Hoppe Grzegorz Leniec Urszula Kowalska The Simple Method of Preparation of Highly Carboxylated Bacterial Cellulose with Ni- and Mg-Ferrite-Based Versatile Magnetic Carrier for Enzyme Immobilization |
topic_facet |
bacterial cellulose magnetic carrier carboxylation citric acid immobilization |
description |
The bacterial cellulose (BC) is a versatile biopolymer of microbial origin characterized by high purity and unusual water and material properties. However, the native BC contains a low number of functional groups, which significantly limits its further application. The main goal of its effective modification is to use methods that allow the unusual properties of BC to be retained and the desired functional group to be efficiently introduced. In the present study, the new magnetic carrier based on functionalized citric acid (CA) bacterial cellulose was developed and tested to support critical industrial enzymes such as lipase B from Candida antarctica and phospholipase A from Aspergillus oryzae. The applied method allowed BC to be effectively modified by citric acid and a sufficient number of carboxylic groups to be introduced, up to 3.6 mmol of COOH per gram of dry mass of the prepared carrier. The DSC and TGA analyses revealed carrier stability at operational temperatures in the range of 20 °C to 100 °C and substantially influenced the amount of the introduced carboxyl groups on carrier properties. Both enzymes’ immobilization significantly improves their thermal stability at 60 °C without a significant thermal and pH optima effect. The analyzed enzymes showed good operational stability with a significant residual activity after ten cycles of repeated uses. The new magnetic carrier based on highly carboxylated bacterial cellulose has a high application capability as matrix for immobilization the various enzymes of industrial interest. |
format |
Text |
author |
Radosław Drozd Magdalena Szymańska Katarzyna Przygrodzka Jakub Hoppe Grzegorz Leniec Urszula Kowalska |
author_facet |
Radosław Drozd Magdalena Szymańska Katarzyna Przygrodzka Jakub Hoppe Grzegorz Leniec Urszula Kowalska |
author_sort |
Radosław Drozd |
title |
The Simple Method of Preparation of Highly Carboxylated Bacterial Cellulose with Ni- and Mg-Ferrite-Based Versatile Magnetic Carrier for Enzyme Immobilization |
title_short |
The Simple Method of Preparation of Highly Carboxylated Bacterial Cellulose with Ni- and Mg-Ferrite-Based Versatile Magnetic Carrier for Enzyme Immobilization |
title_full |
The Simple Method of Preparation of Highly Carboxylated Bacterial Cellulose with Ni- and Mg-Ferrite-Based Versatile Magnetic Carrier for Enzyme Immobilization |
title_fullStr |
The Simple Method of Preparation of Highly Carboxylated Bacterial Cellulose with Ni- and Mg-Ferrite-Based Versatile Magnetic Carrier for Enzyme Immobilization |
title_full_unstemmed |
The Simple Method of Preparation of Highly Carboxylated Bacterial Cellulose with Ni- and Mg-Ferrite-Based Versatile Magnetic Carrier for Enzyme Immobilization |
title_sort |
simple method of preparation of highly carboxylated bacterial cellulose with ni- and mg-ferrite-based versatile magnetic carrier for enzyme immobilization |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2021 |
url |
https://doi.org/10.3390/ijms22168563 |
op_coverage |
agris |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
International Journal of Molecular Sciences; Volume 22; Issue 16; Pages: 8563 |
op_relation |
Macromolecules https://dx.doi.org/10.3390/ijms22168563 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/ijms22168563 |
container_title |
International Journal of Molecular Sciences |
container_volume |
22 |
container_issue |
16 |
container_start_page |
8563 |
_version_ |
1774712793374654464 |