Application of Thin-Layer Chromatography-Flame Ionization Detection (TLC-FID) to Total Lipid Quantitation in Mycolic-Acid Synthesizing Rhodococcus and Williamsia Species

• Published in: International Journal of Molecular Sciences
• Main Authors: Akhikun Nahar, Anthony L. Baker, David S. Nichols, John P. Bowman, Margaret L. Britz
• Format: Text
• Language: English
• Published: Multidisciplinary Digital Publishing Institute 2020
• Subjects: TLC-FID; Iatroscan rods; NMR; lipid extraction; mycolic acids; Williamsia; Rhodococcus; Antarctic; Fid; Antarc*
• Online Access: https://doi.org/10.3390/ijms21051670

In addition to cell membrane phospholipids, Actinobacteria in the order Corynebacteriales possess a waxy cell envelope containing mycolic acids (MA). In optimized culture condition, some species can also accumulate high concentrations of intracellular triacylglycerols (TAG), which are a potential so...