Fusion of Mitochondria to 3-D Networks, Autophagy and Increased Organelle Contacts are Important Subcellular Hallmarks during Cold Stress in Plants
Low temperature stress has a severe impact on the distribution, physiology, and survival of plants in their natural habitats. While numerous studies have focused on the physiological and molecular adjustments to low temperatures, this study provides evidence that cold induced physiological responses...
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ftmdpi:oai:mdpi.com:/1422-0067/21/22/8753/ 2023-08-20T04:09:24+02:00 Fusion of Mitochondria to 3-D Networks, Autophagy and Increased Organelle Contacts are Important Subcellular Hallmarks during Cold Stress in Plants Philip Steiner Othmar Buchner Ancuela Andosch Gerhard Wanner Gilbert Neuner Ursula Lütz-Meindl agris 2020-11-19 application/pdf https://doi.org/10.3390/ijms21228753 EN eng Multidisciplinary Digital Publishing Institute Molecular Plant Sciences https://dx.doi.org/10.3390/ijms21228753 https://creativecommons.org/licenses/by/4.0/ International Journal of Molecular Sciences; Volume 21; Issue 22; Pages: 8753 electron microscopy TEM FIB-SEM Micrasterias denticulata Lemna sp. Ranunculus glacialis organelle networks ultrastructure freezing stress Text 2020 ftmdpi https://doi.org/10.3390/ijms21228753 2023-08-01T00:29:44Z Low temperature stress has a severe impact on the distribution, physiology, and survival of plants in their natural habitats. While numerous studies have focused on the physiological and molecular adjustments to low temperatures, this study provides evidence that cold induced physiological responses coincide with distinct ultrastructural alterations. Three plants from different evolutionary levels and habitats were investigated: The freshwater alga Micrasterias denticulata, the aquatic plant Lemna sp., and the nival plant Ranunculus glacialis. Ultrastructural alterations during low temperature stress were determined by the employment of 2-D transmission electron microscopy and 3-D reconstructions from focused ion beam–scanning electron microscopic series. With decreasing temperatures, increasing numbers of organelle contacts and particularly the fusion of mitochondria to 3-dimensional networks were observed. We assume that the increase or at least maintenance of respiration during low temperature stress is likely to be based on these mitochondrial interconnections. Moreover, it is shown that autophagy and degeneration processes accompany freezing stress in Lemna and R. glacialis. This might be an essential mechanism to recycle damaged cytoplasmic constituents to maintain the cellular metabolism during freezing stress. Text Ranunculus glacialis MDPI Open Access Publishing International Journal of Molecular Sciences 21 22 8753 |
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Open Polar |
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MDPI Open Access Publishing |
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language |
English |
topic |
electron microscopy TEM FIB-SEM Micrasterias denticulata Lemna sp. Ranunculus glacialis organelle networks ultrastructure freezing stress |
spellingShingle |
electron microscopy TEM FIB-SEM Micrasterias denticulata Lemna sp. Ranunculus glacialis organelle networks ultrastructure freezing stress Philip Steiner Othmar Buchner Ancuela Andosch Gerhard Wanner Gilbert Neuner Ursula Lütz-Meindl Fusion of Mitochondria to 3-D Networks, Autophagy and Increased Organelle Contacts are Important Subcellular Hallmarks during Cold Stress in Plants |
topic_facet |
electron microscopy TEM FIB-SEM Micrasterias denticulata Lemna sp. Ranunculus glacialis organelle networks ultrastructure freezing stress |
description |
Low temperature stress has a severe impact on the distribution, physiology, and survival of plants in their natural habitats. While numerous studies have focused on the physiological and molecular adjustments to low temperatures, this study provides evidence that cold induced physiological responses coincide with distinct ultrastructural alterations. Three plants from different evolutionary levels and habitats were investigated: The freshwater alga Micrasterias denticulata, the aquatic plant Lemna sp., and the nival plant Ranunculus glacialis. Ultrastructural alterations during low temperature stress were determined by the employment of 2-D transmission electron microscopy and 3-D reconstructions from focused ion beam–scanning electron microscopic series. With decreasing temperatures, increasing numbers of organelle contacts and particularly the fusion of mitochondria to 3-dimensional networks were observed. We assume that the increase or at least maintenance of respiration during low temperature stress is likely to be based on these mitochondrial interconnections. Moreover, it is shown that autophagy and degeneration processes accompany freezing stress in Lemna and R. glacialis. This might be an essential mechanism to recycle damaged cytoplasmic constituents to maintain the cellular metabolism during freezing stress. |
format |
Text |
author |
Philip Steiner Othmar Buchner Ancuela Andosch Gerhard Wanner Gilbert Neuner Ursula Lütz-Meindl |
author_facet |
Philip Steiner Othmar Buchner Ancuela Andosch Gerhard Wanner Gilbert Neuner Ursula Lütz-Meindl |
author_sort |
Philip Steiner |
title |
Fusion of Mitochondria to 3-D Networks, Autophagy and Increased Organelle Contacts are Important Subcellular Hallmarks during Cold Stress in Plants |
title_short |
Fusion of Mitochondria to 3-D Networks, Autophagy and Increased Organelle Contacts are Important Subcellular Hallmarks during Cold Stress in Plants |
title_full |
Fusion of Mitochondria to 3-D Networks, Autophagy and Increased Organelle Contacts are Important Subcellular Hallmarks during Cold Stress in Plants |
title_fullStr |
Fusion of Mitochondria to 3-D Networks, Autophagy and Increased Organelle Contacts are Important Subcellular Hallmarks during Cold Stress in Plants |
title_full_unstemmed |
Fusion of Mitochondria to 3-D Networks, Autophagy and Increased Organelle Contacts are Important Subcellular Hallmarks during Cold Stress in Plants |
title_sort |
fusion of mitochondria to 3-d networks, autophagy and increased organelle contacts are important subcellular hallmarks during cold stress in plants |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2020 |
url |
https://doi.org/10.3390/ijms21228753 |
op_coverage |
agris |
genre |
Ranunculus glacialis |
genre_facet |
Ranunculus glacialis |
op_source |
International Journal of Molecular Sciences; Volume 21; Issue 22; Pages: 8753 |
op_relation |
Molecular Plant Sciences https://dx.doi.org/10.3390/ijms21228753 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/ijms21228753 |
container_title |
International Journal of Molecular Sciences |
container_volume |
21 |
container_issue |
22 |
container_start_page |
8753 |
_version_ |
1774722341426692096 |