Production of Lipopeptide Biosurfactant by a Hydrocarbon-Degrading Antarctic Rhodococcus
Rhodococci are renowned for their great metabolic repertoire partly because of their numerous putative pathways for large number of specialized metabolites such as biosurfactant. Screening and genome-based assessment for the capacity to produce surface-active molecules was conducted on Rhodococcus s...
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ftmdpi:oai:mdpi.com:/1422-0067/21/17/6138/ 2023-08-20T04:02:34+02:00 Production of Lipopeptide Biosurfactant by a Hydrocarbon-Degrading Antarctic Rhodococcus Syahir Habib Siti Aqlima Ahmad Wan Lutfi Wan Johari Mohd Yunus Abd Shukor Siti Aisyah Alias Jerzy Smykla Nurul Hani Saruni Nur Syafiqah Abdul Razak Nur Adeela Yasid agris 2020-08-26 application/pdf https://doi.org/10.3390/ijms21176138 EN eng Multidisciplinary Digital Publishing Institute Molecular Informatics https://dx.doi.org/10.3390/ijms21176138 https://creativecommons.org/licenses/by/4.0/ International Journal of Molecular Sciences; Volume 21; Issue 17; Pages: 6138 surface-active lipopeptide biosynthetic gene clusters fellfield soil Antarctica Text 2020 ftmdpi https://doi.org/10.3390/ijms21176138 2023-07-31T23:59:03Z Rhodococci are renowned for their great metabolic repertoire partly because of their numerous putative pathways for large number of specialized metabolites such as biosurfactant. Screening and genome-based assessment for the capacity to produce surface-active molecules was conducted on Rhodococcus sp. ADL36, a diesel-degrading Antarctic bacterium. The strain showed a positive bacterial adhesion to hydrocarbon (BATH) assay, drop collapse test, oil displacement activity, microplate assay, maximal emulsification index at 45% and ability to reduce water surface tension to < 30 mN/m. The evaluation of the cell-free supernatant demonstrated its high stability across the temperature, pH and salinity gradient although no correlation was found between the surface and emulsification activity. Based on the positive relationship between the assessment of macromolecules content and infrared analysis, the extracted biosurfactant synthesized was classified as a lipopeptide. Prediction of the secondary metabolites in the non-ribosomal peptide synthetase (NRPS) clusters suggested the likelihood of the surface-active lipopeptide production in the strain’s genomic data. This is the third report of surface-active lipopeptide producers from this phylotype and the first from the polar region. The lipopeptide synthesized by ADL36 has the prospect to be an Antarctic remediation tool while furnishing a distinctive natural product for biotechnological application and research. Text Antarc* Antarctic Antarctica MDPI Open Access Publishing Antarctic International Journal of Molecular Sciences 21 17 6138 |
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Open Polar |
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MDPI Open Access Publishing |
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ftmdpi |
language |
English |
topic |
surface-active lipopeptide biosynthetic gene clusters fellfield soil Antarctica |
spellingShingle |
surface-active lipopeptide biosynthetic gene clusters fellfield soil Antarctica Syahir Habib Siti Aqlima Ahmad Wan Lutfi Wan Johari Mohd Yunus Abd Shukor Siti Aisyah Alias Jerzy Smykla Nurul Hani Saruni Nur Syafiqah Abdul Razak Nur Adeela Yasid Production of Lipopeptide Biosurfactant by a Hydrocarbon-Degrading Antarctic Rhodococcus |
topic_facet |
surface-active lipopeptide biosynthetic gene clusters fellfield soil Antarctica |
description |
Rhodococci are renowned for their great metabolic repertoire partly because of their numerous putative pathways for large number of specialized metabolites such as biosurfactant. Screening and genome-based assessment for the capacity to produce surface-active molecules was conducted on Rhodococcus sp. ADL36, a diesel-degrading Antarctic bacterium. The strain showed a positive bacterial adhesion to hydrocarbon (BATH) assay, drop collapse test, oil displacement activity, microplate assay, maximal emulsification index at 45% and ability to reduce water surface tension to < 30 mN/m. The evaluation of the cell-free supernatant demonstrated its high stability across the temperature, pH and salinity gradient although no correlation was found between the surface and emulsification activity. Based on the positive relationship between the assessment of macromolecules content and infrared analysis, the extracted biosurfactant synthesized was classified as a lipopeptide. Prediction of the secondary metabolites in the non-ribosomal peptide synthetase (NRPS) clusters suggested the likelihood of the surface-active lipopeptide production in the strain’s genomic data. This is the third report of surface-active lipopeptide producers from this phylotype and the first from the polar region. The lipopeptide synthesized by ADL36 has the prospect to be an Antarctic remediation tool while furnishing a distinctive natural product for biotechnological application and research. |
format |
Text |
author |
Syahir Habib Siti Aqlima Ahmad Wan Lutfi Wan Johari Mohd Yunus Abd Shukor Siti Aisyah Alias Jerzy Smykla Nurul Hani Saruni Nur Syafiqah Abdul Razak Nur Adeela Yasid |
author_facet |
Syahir Habib Siti Aqlima Ahmad Wan Lutfi Wan Johari Mohd Yunus Abd Shukor Siti Aisyah Alias Jerzy Smykla Nurul Hani Saruni Nur Syafiqah Abdul Razak Nur Adeela Yasid |
author_sort |
Syahir Habib |
title |
Production of Lipopeptide Biosurfactant by a Hydrocarbon-Degrading Antarctic Rhodococcus |
title_short |
Production of Lipopeptide Biosurfactant by a Hydrocarbon-Degrading Antarctic Rhodococcus |
title_full |
Production of Lipopeptide Biosurfactant by a Hydrocarbon-Degrading Antarctic Rhodococcus |
title_fullStr |
Production of Lipopeptide Biosurfactant by a Hydrocarbon-Degrading Antarctic Rhodococcus |
title_full_unstemmed |
Production of Lipopeptide Biosurfactant by a Hydrocarbon-Degrading Antarctic Rhodococcus |
title_sort |
production of lipopeptide biosurfactant by a hydrocarbon-degrading antarctic rhodococcus |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2020 |
url |
https://doi.org/10.3390/ijms21176138 |
op_coverage |
agris |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_source |
International Journal of Molecular Sciences; Volume 21; Issue 17; Pages: 6138 |
op_relation |
Molecular Informatics https://dx.doi.org/10.3390/ijms21176138 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/ijms21176138 |
container_title |
International Journal of Molecular Sciences |
container_volume |
21 |
container_issue |
17 |
container_start_page |
6138 |
_version_ |
1774713079311892480 |