Ethyl Butyrate Synthesis Catalyzed by Lipases A and B from Candida antarctica Immobilized onto Magnetic Nanoparticles. Improvement of Biocatalysts’ Performance under Ultrasonic Irradiation
The synthesis of ethyl butyrate catalyzed by lipases A (CALA) or B (CALB) from Candida antarctica immobilized onto magnetic nanoparticles (MNP), CALA-MNP and CALB-MNP, respectively, is hereby reported. MNPs were prepared by co-precipitation, functionalized with 3-aminopropyltriethoxysilane, activate...
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ftmdpi:oai:mdpi.com:/1422-0067/20/22/5807/ 2023-08-20T04:02:31+02:00 Ethyl Butyrate Synthesis Catalyzed by Lipases A and B from Candida antarctica Immobilized onto Magnetic Nanoparticles. Improvement of Biocatalysts’ Performance under Ultrasonic Irradiation Rodolpho R. C. Monteiro Davino M. Andrade Neto Pierre B. A. Fechine Ada A. S. Lopes Luciana R. B. Gonçalves José C. S. dos Santos Maria C. M. de Souza Roberto Fernandez-Lafuente agris 2019-11-19 application/pdf https://doi.org/10.3390/ijms20225807 EN eng Multidisciplinary Digital Publishing Institute Materials Science https://dx.doi.org/10.3390/ijms20225807 https://creativecommons.org/licenses/by/4.0/ International Journal of Molecular Sciences; Volume 20; Issue 22; Pages: 5807 ethyl butyrate lipase A from Candida antarctica lipase B from Candida antarctica magnetic nanoparticles ultrasonic irradiation Text 2019 ftmdpi https://doi.org/10.3390/ijms20225807 2023-07-31T22:48:30Z The synthesis of ethyl butyrate catalyzed by lipases A (CALA) or B (CALB) from Candida antarctica immobilized onto magnetic nanoparticles (MNP), CALA-MNP and CALB-MNP, respectively, is hereby reported. MNPs were prepared by co-precipitation, functionalized with 3-aminopropyltriethoxysilane, activated with glutaraldehyde, and then used as support to immobilize either CALA or CALB (immobilization yield: 100 ± 1.2% and 57.6 ± 3.8%; biocatalysts activities: 198.3 ± 2.7 Up-NPB/g and 52.9 ± 1.7 Up-NPB/g for CALA-MNP and CALB-MNP, respectively). X-ray diffraction and Raman spectroscopy analysis indicated the production of a magnetic nanomaterial with a diameter of 13.0 nm, whereas Fourier-transform infrared spectroscopy indicated functionalization, activation and enzyme immobilization. To determine the optimum conditions for the synthesis, a four-variable Central Composite Design (CCD) (biocatalyst content, molar ratio, temperature and time) was performed. Under optimized conditions (1:1, 45 °C and 6 h), it was possible to achieve 99.2 ± 0.3% of conversion for CALA-MNP (10 mg) and 97.5 ± 0.8% for CALB-MNP (12.5 mg), which retained approximately 80% of their activity after 10 consecutive cycles of esterification. Under ultrasonic irradiation, similar conversions were achieved but at 4 h of incubation, demonstrating the efficiency of ultrasound technology in the enzymatic synthesis of esters. Text Antarc* Antarctica MDPI Open Access Publishing International Journal of Molecular Sciences 20 22 5807 |
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Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
ethyl butyrate lipase A from Candida antarctica lipase B from Candida antarctica magnetic nanoparticles ultrasonic irradiation |
spellingShingle |
ethyl butyrate lipase A from Candida antarctica lipase B from Candida antarctica magnetic nanoparticles ultrasonic irradiation Rodolpho R. C. Monteiro Davino M. Andrade Neto Pierre B. A. Fechine Ada A. S. Lopes Luciana R. B. Gonçalves José C. S. dos Santos Maria C. M. de Souza Roberto Fernandez-Lafuente Ethyl Butyrate Synthesis Catalyzed by Lipases A and B from Candida antarctica Immobilized onto Magnetic Nanoparticles. Improvement of Biocatalysts’ Performance under Ultrasonic Irradiation |
topic_facet |
ethyl butyrate lipase A from Candida antarctica lipase B from Candida antarctica magnetic nanoparticles ultrasonic irradiation |
description |
The synthesis of ethyl butyrate catalyzed by lipases A (CALA) or B (CALB) from Candida antarctica immobilized onto magnetic nanoparticles (MNP), CALA-MNP and CALB-MNP, respectively, is hereby reported. MNPs were prepared by co-precipitation, functionalized with 3-aminopropyltriethoxysilane, activated with glutaraldehyde, and then used as support to immobilize either CALA or CALB (immobilization yield: 100 ± 1.2% and 57.6 ± 3.8%; biocatalysts activities: 198.3 ± 2.7 Up-NPB/g and 52.9 ± 1.7 Up-NPB/g for CALA-MNP and CALB-MNP, respectively). X-ray diffraction and Raman spectroscopy analysis indicated the production of a magnetic nanomaterial with a diameter of 13.0 nm, whereas Fourier-transform infrared spectroscopy indicated functionalization, activation and enzyme immobilization. To determine the optimum conditions for the synthesis, a four-variable Central Composite Design (CCD) (biocatalyst content, molar ratio, temperature and time) was performed. Under optimized conditions (1:1, 45 °C and 6 h), it was possible to achieve 99.2 ± 0.3% of conversion for CALA-MNP (10 mg) and 97.5 ± 0.8% for CALB-MNP (12.5 mg), which retained approximately 80% of their activity after 10 consecutive cycles of esterification. Under ultrasonic irradiation, similar conversions were achieved but at 4 h of incubation, demonstrating the efficiency of ultrasound technology in the enzymatic synthesis of esters. |
format |
Text |
author |
Rodolpho R. C. Monteiro Davino M. Andrade Neto Pierre B. A. Fechine Ada A. S. Lopes Luciana R. B. Gonçalves José C. S. dos Santos Maria C. M. de Souza Roberto Fernandez-Lafuente |
author_facet |
Rodolpho R. C. Monteiro Davino M. Andrade Neto Pierre B. A. Fechine Ada A. S. Lopes Luciana R. B. Gonçalves José C. S. dos Santos Maria C. M. de Souza Roberto Fernandez-Lafuente |
author_sort |
Rodolpho R. C. Monteiro |
title |
Ethyl Butyrate Synthesis Catalyzed by Lipases A and B from Candida antarctica Immobilized onto Magnetic Nanoparticles. Improvement of Biocatalysts’ Performance under Ultrasonic Irradiation |
title_short |
Ethyl Butyrate Synthesis Catalyzed by Lipases A and B from Candida antarctica Immobilized onto Magnetic Nanoparticles. Improvement of Biocatalysts’ Performance under Ultrasonic Irradiation |
title_full |
Ethyl Butyrate Synthesis Catalyzed by Lipases A and B from Candida antarctica Immobilized onto Magnetic Nanoparticles. Improvement of Biocatalysts’ Performance under Ultrasonic Irradiation |
title_fullStr |
Ethyl Butyrate Synthesis Catalyzed by Lipases A and B from Candida antarctica Immobilized onto Magnetic Nanoparticles. Improvement of Biocatalysts’ Performance under Ultrasonic Irradiation |
title_full_unstemmed |
Ethyl Butyrate Synthesis Catalyzed by Lipases A and B from Candida antarctica Immobilized onto Magnetic Nanoparticles. Improvement of Biocatalysts’ Performance under Ultrasonic Irradiation |
title_sort |
ethyl butyrate synthesis catalyzed by lipases a and b from candida antarctica immobilized onto magnetic nanoparticles. improvement of biocatalysts’ performance under ultrasonic irradiation |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2019 |
url |
https://doi.org/10.3390/ijms20225807 |
op_coverage |
agris |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
International Journal of Molecular Sciences; Volume 20; Issue 22; Pages: 5807 |
op_relation |
Materials Science https://dx.doi.org/10.3390/ijms20225807 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/ijms20225807 |
container_title |
International Journal of Molecular Sciences |
container_volume |
20 |
container_issue |
22 |
container_start_page |
5807 |
_version_ |
1774713004353388544 |