In Silico Analysis of Pacific Oyster (Crassostrea gigas) Transcriptome over Developmental Stages Reveals Candidate Genes for Larval Settlement
Following their planktonic phase, the larvae of benthic marine organisms must locate a suitable habitat to settle and metamorphose. For oysters, larval adhesion occurs at the pediveliger stage with the secretion of a proteinaceous bioadhesive produced by the foot, a specialized and ephemeral organ....
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ftmdpi:oai:mdpi.com:/1422-0067/20/1/197/ 2023-08-20T04:06:01+02:00 In Silico Analysis of Pacific Oyster (Crassostrea gigas) Transcriptome over Developmental Stages Reveals Candidate Genes for Larval Settlement Valentin Foulon Pierre Boudry Sébastien Artigaud Fabienne Guérard Claire Hellio agris 2019-01-08 application/pdf https://doi.org/10.3390/ijms20010197 EN eng Multidisciplinary Digital Publishing Institute Molecular Biology https://dx.doi.org/10.3390/ijms20010197 https://creativecommons.org/licenses/by/4.0/ International Journal of Molecular Sciences; Volume 20; Issue 1; Pages: 197 Crassostrea gigas Pacific oyster pediveliger larvae bioadhesive transcriptome Text 2019 ftmdpi https://doi.org/10.3390/ijms20010197 2023-07-31T21:57:10Z Following their planktonic phase, the larvae of benthic marine organisms must locate a suitable habitat to settle and metamorphose. For oysters, larval adhesion occurs at the pediveliger stage with the secretion of a proteinaceous bioadhesive produced by the foot, a specialized and ephemeral organ. Oyster bioadhesive is highly resistant to proteomic extraction and is only produced in very low quantities, which explains why it has been very little examined in larvae to date. In silico analysis of nucleic acid databases could help to identify genes of interest implicated in settlement. In this work, the publicly available transcriptome of Pacific oyster Crassostrea gigas over its developmental stages was mined to select genes highly expressed at the pediveliger stage. Our analysis revealed 59 sequences potentially implicated in adhesion of C. gigas larvae. Some related proteins contain conserved domains already described in other bioadhesives. We propose a hypothetic composition of C. gigas bioadhesive in which the protein constituent is probably composed of collagen and the von Willebrand Factor domain could play a role in adhesive cohesion. Genes coding for enzymes implicated in DOPA chemistry were also detected, indicating that this modification is also potentially present in the adhesive of pediveliger larvae. Text Crassostrea gigas Pacific oyster MDPI Open Access Publishing Pacific International Journal of Molecular Sciences 20 1 197 |
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Open Polar |
collection |
MDPI Open Access Publishing |
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ftmdpi |
language |
English |
topic |
Crassostrea gigas Pacific oyster pediveliger larvae bioadhesive transcriptome |
spellingShingle |
Crassostrea gigas Pacific oyster pediveliger larvae bioadhesive transcriptome Valentin Foulon Pierre Boudry Sébastien Artigaud Fabienne Guérard Claire Hellio In Silico Analysis of Pacific Oyster (Crassostrea gigas) Transcriptome over Developmental Stages Reveals Candidate Genes for Larval Settlement |
topic_facet |
Crassostrea gigas Pacific oyster pediveliger larvae bioadhesive transcriptome |
description |
Following their planktonic phase, the larvae of benthic marine organisms must locate a suitable habitat to settle and metamorphose. For oysters, larval adhesion occurs at the pediveliger stage with the secretion of a proteinaceous bioadhesive produced by the foot, a specialized and ephemeral organ. Oyster bioadhesive is highly resistant to proteomic extraction and is only produced in very low quantities, which explains why it has been very little examined in larvae to date. In silico analysis of nucleic acid databases could help to identify genes of interest implicated in settlement. In this work, the publicly available transcriptome of Pacific oyster Crassostrea gigas over its developmental stages was mined to select genes highly expressed at the pediveliger stage. Our analysis revealed 59 sequences potentially implicated in adhesion of C. gigas larvae. Some related proteins contain conserved domains already described in other bioadhesives. We propose a hypothetic composition of C. gigas bioadhesive in which the protein constituent is probably composed of collagen and the von Willebrand Factor domain could play a role in adhesive cohesion. Genes coding for enzymes implicated in DOPA chemistry were also detected, indicating that this modification is also potentially present in the adhesive of pediveliger larvae. |
format |
Text |
author |
Valentin Foulon Pierre Boudry Sébastien Artigaud Fabienne Guérard Claire Hellio |
author_facet |
Valentin Foulon Pierre Boudry Sébastien Artigaud Fabienne Guérard Claire Hellio |
author_sort |
Valentin Foulon |
title |
In Silico Analysis of Pacific Oyster (Crassostrea gigas) Transcriptome over Developmental Stages Reveals Candidate Genes for Larval Settlement |
title_short |
In Silico Analysis of Pacific Oyster (Crassostrea gigas) Transcriptome over Developmental Stages Reveals Candidate Genes for Larval Settlement |
title_full |
In Silico Analysis of Pacific Oyster (Crassostrea gigas) Transcriptome over Developmental Stages Reveals Candidate Genes for Larval Settlement |
title_fullStr |
In Silico Analysis of Pacific Oyster (Crassostrea gigas) Transcriptome over Developmental Stages Reveals Candidate Genes for Larval Settlement |
title_full_unstemmed |
In Silico Analysis of Pacific Oyster (Crassostrea gigas) Transcriptome over Developmental Stages Reveals Candidate Genes for Larval Settlement |
title_sort |
in silico analysis of pacific oyster (crassostrea gigas) transcriptome over developmental stages reveals candidate genes for larval settlement |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2019 |
url |
https://doi.org/10.3390/ijms20010197 |
op_coverage |
agris |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
International Journal of Molecular Sciences; Volume 20; Issue 1; Pages: 197 |
op_relation |
Molecular Biology https://dx.doi.org/10.3390/ijms20010197 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/ijms20010197 |
container_title |
International Journal of Molecular Sciences |
container_volume |
20 |
container_issue |
1 |
container_start_page |
197 |
_version_ |
1774716919659626496 |