Adjuvant Effect of Quillaja saponaria Saponin (QSS) on Protective Efficacy and IgM Generation in Turbot (Scophthalmus maximus) upon Immersion Vaccination
The adjuvant effect of Quillaja saponaria saponin (QSS) on protection of turbot fry was investigated with immersion vaccination of formalin-killed Vibrio anguillarum O1 and various concentrations of QSS (5, 25, 45 and 65 mg/L). Fish were challenged at days 7, 14 and 28 post-vaccination. Significantl...
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ftmdpi:oai:mdpi.com:/1422-0067/17/3/325/ 2023-08-20T04:09:40+02:00 Adjuvant Effect of Quillaja saponaria Saponin (QSS) on Protective Efficacy and IgM Generation in Turbot (Scophthalmus maximus) upon Immersion Vaccination Yujuan Wang Xiuhua Wang Jie Huang Jun Li agris 2016-03-02 application/pdf https://doi.org/10.3390/ijms17030325 EN eng Multidisciplinary Digital Publishing Institute Biochemistry https://dx.doi.org/10.3390/ijms17030325 https://creativecommons.org/licenses/by/4.0/ International Journal of Molecular Sciences; Volume 17; Issue 3; Pages: 325 Quillaja saponaria saponins adjuvant vaccination Scophthalmus maximus relative percent of survival (RPS) Text 2016 ftmdpi https://doi.org/10.3390/ijms17030325 2023-07-31T20:50:50Z The adjuvant effect of Quillaja saponaria saponin (QSS) on protection of turbot fry was investigated with immersion vaccination of formalin-killed Vibrio anguillarum O1 and various concentrations of QSS (5, 25, 45 and 65 mg/L). Fish were challenged at days 7, 14 and 28 post-vaccination. Significantly high relative percent of survival (RPS) ((59.1 ± 13.6)%, (81.7 ± 8.2)%, (77.8 ± 9.6)%) were recorded in the fish that received bacterins immersion with QSS at 45 mg/L, which is comparable to the positive control group vaccinated by intraperitoneal injection (IP). Moreover, a remarkably higher serum antibody titer was also demonstrated after 28 days in the vaccinated fish with QSS (45 mg/L) than those vaccinated fish without QSS (p < 0.05), but lower than the IP immunized fish (p < 0.05). Significant upregulation of IgM gene expression has also been identified in the tissues of skin, gill, spleen and kidney from the immunized fish in comparison to the control fish. Taken together, the present study indicated that QSS was able to dramatically evoke systemic and mucosal immune responses in immunized fish. Therefore, QSS might be a promising adjuvant candidate for fish vaccination via an immersion administering route. Text Scophthalmus maximus Turbot MDPI Open Access Publishing International Journal of Molecular Sciences 17 3 325 |
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English |
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Quillaja saponaria saponins adjuvant vaccination Scophthalmus maximus relative percent of survival (RPS) |
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Quillaja saponaria saponins adjuvant vaccination Scophthalmus maximus relative percent of survival (RPS) Yujuan Wang Xiuhua Wang Jie Huang Jun Li Adjuvant Effect of Quillaja saponaria Saponin (QSS) on Protective Efficacy and IgM Generation in Turbot (Scophthalmus maximus) upon Immersion Vaccination |
topic_facet |
Quillaja saponaria saponins adjuvant vaccination Scophthalmus maximus relative percent of survival (RPS) |
description |
The adjuvant effect of Quillaja saponaria saponin (QSS) on protection of turbot fry was investigated with immersion vaccination of formalin-killed Vibrio anguillarum O1 and various concentrations of QSS (5, 25, 45 and 65 mg/L). Fish were challenged at days 7, 14 and 28 post-vaccination. Significantly high relative percent of survival (RPS) ((59.1 ± 13.6)%, (81.7 ± 8.2)%, (77.8 ± 9.6)%) were recorded in the fish that received bacterins immersion with QSS at 45 mg/L, which is comparable to the positive control group vaccinated by intraperitoneal injection (IP). Moreover, a remarkably higher serum antibody titer was also demonstrated after 28 days in the vaccinated fish with QSS (45 mg/L) than those vaccinated fish without QSS (p < 0.05), but lower than the IP immunized fish (p < 0.05). Significant upregulation of IgM gene expression has also been identified in the tissues of skin, gill, spleen and kidney from the immunized fish in comparison to the control fish. Taken together, the present study indicated that QSS was able to dramatically evoke systemic and mucosal immune responses in immunized fish. Therefore, QSS might be a promising adjuvant candidate for fish vaccination via an immersion administering route. |
format |
Text |
author |
Yujuan Wang Xiuhua Wang Jie Huang Jun Li |
author_facet |
Yujuan Wang Xiuhua Wang Jie Huang Jun Li |
author_sort |
Yujuan Wang |
title |
Adjuvant Effect of Quillaja saponaria Saponin (QSS) on Protective Efficacy and IgM Generation in Turbot (Scophthalmus maximus) upon Immersion Vaccination |
title_short |
Adjuvant Effect of Quillaja saponaria Saponin (QSS) on Protective Efficacy and IgM Generation in Turbot (Scophthalmus maximus) upon Immersion Vaccination |
title_full |
Adjuvant Effect of Quillaja saponaria Saponin (QSS) on Protective Efficacy and IgM Generation in Turbot (Scophthalmus maximus) upon Immersion Vaccination |
title_fullStr |
Adjuvant Effect of Quillaja saponaria Saponin (QSS) on Protective Efficacy and IgM Generation in Turbot (Scophthalmus maximus) upon Immersion Vaccination |
title_full_unstemmed |
Adjuvant Effect of Quillaja saponaria Saponin (QSS) on Protective Efficacy and IgM Generation in Turbot (Scophthalmus maximus) upon Immersion Vaccination |
title_sort |
adjuvant effect of quillaja saponaria saponin (qss) on protective efficacy and igm generation in turbot (scophthalmus maximus) upon immersion vaccination |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2016 |
url |
https://doi.org/10.3390/ijms17030325 |
op_coverage |
agris |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_source |
International Journal of Molecular Sciences; Volume 17; Issue 3; Pages: 325 |
op_relation |
Biochemistry https://dx.doi.org/10.3390/ijms17030325 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/ijms17030325 |
container_title |
International Journal of Molecular Sciences |
container_volume |
17 |
container_issue |
3 |
container_start_page |
325 |
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1774723267511189504 |