Identification and Evaluation of 21 Novel Microsatellite Markers from the Autumnal Moth (Epirrita autumnata) (Lepidoptera: Geometridae)
The autumnal moth (Epirrita autumnata) is a cyclically outbreaking forest Lepidoptera with circumpolar distribution and substantial impact on Northern ecosystems. We have isolated 21 microsatellites from the species to facilitate population genetic studies of population cycles, outbreaks, and crashe...
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ftmdpi:oai:mdpi.com:/1422-0067/16/9/22541/ 2023-08-20T04:08:45+02:00 Identification and Evaluation of 21 Novel Microsatellite Markers from the Autumnal Moth (Epirrita autumnata) (Lepidoptera: Geometridae) Siv Aarnes Ida Fløystad Julia Schregel Ole Vindstad Jane Jepsen Hans Eiken Rolf Ims Snorre Hagen agris 2015-09-17 application/pdf https://doi.org/10.3390/ijms160922541 EN eng Multidisciplinary Digital Publishing Institute Biochemistry https://dx.doi.org/10.3390/ijms160922541 https://creativecommons.org/licenses/by/4.0/ International Journal of Molecular Sciences; Volume 16; Issue 9; Pages: 22541-22554 tri- and tetranucleotide microsatellites multiplex PCR Lepidoptera population genetics Text 2015 ftmdpi https://doi.org/10.3390/ijms160922541 2023-07-31T20:46:35Z The autumnal moth (Epirrita autumnata) is a cyclically outbreaking forest Lepidoptera with circumpolar distribution and substantial impact on Northern ecosystems. We have isolated 21 microsatellites from the species to facilitate population genetic studies of population cycles, outbreaks, and crashes. First, PCR primers and PCR conditions were developed to amplify 19 trinucleotide loci and two tetranucleotide loci in six multiplex PCR approaches and then analyzed for species specificity, sensitivity and precision. Twelve of the loci showed simple tandem repeat array structures while nine loci showed imperfect repeat structures, and repeat numbers varied in our material between six and 15. The application in population genetics for all the 21 microsatellites were further validated in 48 autumnal moths sampled from Northern Norway, and allelic variation was detected in 19 loci. The detected numbers of alleles per locus ranged from two to 13, and the observed and expected heterozygosities varied from 0.04 to 0.69 and 0.04 to 0.79, respectively. Evidence for linkage disequilibrium was found for six loci as well as indication of one null allele. We find that these novel microsatellites and their multiplex-PCR assays are suitable for further research on fine- and large-scale population-genetic studies of Epirrita autumnata. Text Northern Norway MDPI Open Access Publishing Norway International Journal of Molecular Sciences 16 9 22541 22554 |
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MDPI Open Access Publishing |
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ftmdpi |
language |
English |
topic |
tri- and tetranucleotide microsatellites multiplex PCR Lepidoptera population genetics |
spellingShingle |
tri- and tetranucleotide microsatellites multiplex PCR Lepidoptera population genetics Siv Aarnes Ida Fløystad Julia Schregel Ole Vindstad Jane Jepsen Hans Eiken Rolf Ims Snorre Hagen Identification and Evaluation of 21 Novel Microsatellite Markers from the Autumnal Moth (Epirrita autumnata) (Lepidoptera: Geometridae) |
topic_facet |
tri- and tetranucleotide microsatellites multiplex PCR Lepidoptera population genetics |
description |
The autumnal moth (Epirrita autumnata) is a cyclically outbreaking forest Lepidoptera with circumpolar distribution and substantial impact on Northern ecosystems. We have isolated 21 microsatellites from the species to facilitate population genetic studies of population cycles, outbreaks, and crashes. First, PCR primers and PCR conditions were developed to amplify 19 trinucleotide loci and two tetranucleotide loci in six multiplex PCR approaches and then analyzed for species specificity, sensitivity and precision. Twelve of the loci showed simple tandem repeat array structures while nine loci showed imperfect repeat structures, and repeat numbers varied in our material between six and 15. The application in population genetics for all the 21 microsatellites were further validated in 48 autumnal moths sampled from Northern Norway, and allelic variation was detected in 19 loci. The detected numbers of alleles per locus ranged from two to 13, and the observed and expected heterozygosities varied from 0.04 to 0.69 and 0.04 to 0.79, respectively. Evidence for linkage disequilibrium was found for six loci as well as indication of one null allele. We find that these novel microsatellites and their multiplex-PCR assays are suitable for further research on fine- and large-scale population-genetic studies of Epirrita autumnata. |
format |
Text |
author |
Siv Aarnes Ida Fløystad Julia Schregel Ole Vindstad Jane Jepsen Hans Eiken Rolf Ims Snorre Hagen |
author_facet |
Siv Aarnes Ida Fløystad Julia Schregel Ole Vindstad Jane Jepsen Hans Eiken Rolf Ims Snorre Hagen |
author_sort |
Siv Aarnes |
title |
Identification and Evaluation of 21 Novel Microsatellite Markers from the Autumnal Moth (Epirrita autumnata) (Lepidoptera: Geometridae) |
title_short |
Identification and Evaluation of 21 Novel Microsatellite Markers from the Autumnal Moth (Epirrita autumnata) (Lepidoptera: Geometridae) |
title_full |
Identification and Evaluation of 21 Novel Microsatellite Markers from the Autumnal Moth (Epirrita autumnata) (Lepidoptera: Geometridae) |
title_fullStr |
Identification and Evaluation of 21 Novel Microsatellite Markers from the Autumnal Moth (Epirrita autumnata) (Lepidoptera: Geometridae) |
title_full_unstemmed |
Identification and Evaluation of 21 Novel Microsatellite Markers from the Autumnal Moth (Epirrita autumnata) (Lepidoptera: Geometridae) |
title_sort |
identification and evaluation of 21 novel microsatellite markers from the autumnal moth (epirrita autumnata) (lepidoptera: geometridae) |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2015 |
url |
https://doi.org/10.3390/ijms160922541 |
op_coverage |
agris |
geographic |
Norway |
geographic_facet |
Norway |
genre |
Northern Norway |
genre_facet |
Northern Norway |
op_source |
International Journal of Molecular Sciences; Volume 16; Issue 9; Pages: 22541-22554 |
op_relation |
Biochemistry https://dx.doi.org/10.3390/ijms160922541 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/ijms160922541 |
container_title |
International Journal of Molecular Sciences |
container_volume |
16 |
container_issue |
9 |
container_start_page |
22541 |
op_container_end_page |
22554 |
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1774721216046694400 |