Tissue Localization of Lymphocystis Disease Virus (LCDV) Receptor-27.8 kDa and Its Expression Kinetics Induced by the Viral Infection in Turbot (Scophthalmus maximus)
The 27.8 kDa membrane protein expressed in flounder (Paralichthys olivaceus) gill cells was proved to be a receptor mediating lymphocystis disease virus (LCDV) infection. In this study, SDS-PAGE and Western blotting demonstrated that 27.8 kDa receptor (27.8R) was shared by flounder and turbot (Scoph...
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ftmdpi:oai:mdpi.com:/1422-0067/16/11/26506/ 2023-05-15T18:15:40+02:00 Tissue Localization of Lymphocystis Disease Virus (LCDV) Receptor-27.8 kDa and Its Expression Kinetics Induced by the Viral Infection in Turbot (Scophthalmus maximus) Xiuzhen Sheng Ronghua Wu Xiaoqian Tang Jing Xing Wenbin Zhan 2015-11-05 application/pdf https://doi.org/10.3390/ijms161125974 EN eng Multidisciplinary Digital Publishing Institute Biochemistry, Molecular and Cellular Biology http://creativecommons.org/licenses/by/3.0/ CC-BY International Journal of Molecular Sciences Volume 16 Issue 11 Pages 26506-26519 lymphocystis disease virus turbot ( Scophthalmus maximus ) receptor-27.8 kDa expression dynamics tissue localization Text 2015 ftmdpi https://doi.org/10.3390/ijms161125974 2019-04-08T21:52:00Z The 27.8 kDa membrane protein expressed in flounder (Paralichthys olivaceus) gill cells was proved to be a receptor mediating lymphocystis disease virus (LCDV) infection. In this study, SDS-PAGE and Western blotting demonstrated that 27.8 kDa receptor (27.8R) was shared by flounder and turbot (Scophthalmus maximus). Indirect immunofluorescence assay (IIFA) and immunohistochemistry showed that 27.8R was widely expressed in tested tissues of healthy turbot. The indirect enzyme-linked immunosorbent assay indicated that 27.8R expression was relatively higher in stomach, gill, heart, and intestine, followed by skin, head kidney, spleen, blood cells, kidney and liver, and lower in ovary and brain in healthy turbot, and it was significantly up-regulated after LCDV infection. Meanwhile, real-time quantitative PCR demonstrated that LCDV was detected in heart, peripheral blood cells, and head kidney at 3 h post infection (p.i.), and then in other tested tissues at 12 h p.i. LCDV copies increased in a time-dependent manner, and were generally higher in the tissues with higher 27.8R expression. Additionally, IIFA showed that 27.8R and LCDV were detected at 3 h p.i. in some leukocytes. These results suggested that 27.8R also served as a receptor in turbot, and LCDV can infect some leukocytes which might result in LCDV spreading to different tissues in turbot. Text Scophthalmus maximus Turbot MDPI Open Access Publishing International Journal of Molecular Sciences 16 11 26506 26519 |
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English |
topic |
lymphocystis disease virus turbot ( Scophthalmus maximus ) receptor-27.8 kDa expression dynamics tissue localization |
spellingShingle |
lymphocystis disease virus turbot ( Scophthalmus maximus ) receptor-27.8 kDa expression dynamics tissue localization Xiuzhen Sheng Ronghua Wu Xiaoqian Tang Jing Xing Wenbin Zhan Tissue Localization of Lymphocystis Disease Virus (LCDV) Receptor-27.8 kDa and Its Expression Kinetics Induced by the Viral Infection in Turbot (Scophthalmus maximus) |
topic_facet |
lymphocystis disease virus turbot ( Scophthalmus maximus ) receptor-27.8 kDa expression dynamics tissue localization |
description |
The 27.8 kDa membrane protein expressed in flounder (Paralichthys olivaceus) gill cells was proved to be a receptor mediating lymphocystis disease virus (LCDV) infection. In this study, SDS-PAGE and Western blotting demonstrated that 27.8 kDa receptor (27.8R) was shared by flounder and turbot (Scophthalmus maximus). Indirect immunofluorescence assay (IIFA) and immunohistochemistry showed that 27.8R was widely expressed in tested tissues of healthy turbot. The indirect enzyme-linked immunosorbent assay indicated that 27.8R expression was relatively higher in stomach, gill, heart, and intestine, followed by skin, head kidney, spleen, blood cells, kidney and liver, and lower in ovary and brain in healthy turbot, and it was significantly up-regulated after LCDV infection. Meanwhile, real-time quantitative PCR demonstrated that LCDV was detected in heart, peripheral blood cells, and head kidney at 3 h post infection (p.i.), and then in other tested tissues at 12 h p.i. LCDV copies increased in a time-dependent manner, and were generally higher in the tissues with higher 27.8R expression. Additionally, IIFA showed that 27.8R and LCDV were detected at 3 h p.i. in some leukocytes. These results suggested that 27.8R also served as a receptor in turbot, and LCDV can infect some leukocytes which might result in LCDV spreading to different tissues in turbot. |
format |
Text |
author |
Xiuzhen Sheng Ronghua Wu Xiaoqian Tang Jing Xing Wenbin Zhan |
author_facet |
Xiuzhen Sheng Ronghua Wu Xiaoqian Tang Jing Xing Wenbin Zhan |
author_sort |
Xiuzhen Sheng |
title |
Tissue Localization of Lymphocystis Disease Virus (LCDV) Receptor-27.8 kDa and Its Expression Kinetics Induced by the Viral Infection in Turbot (Scophthalmus maximus) |
title_short |
Tissue Localization of Lymphocystis Disease Virus (LCDV) Receptor-27.8 kDa and Its Expression Kinetics Induced by the Viral Infection in Turbot (Scophthalmus maximus) |
title_full |
Tissue Localization of Lymphocystis Disease Virus (LCDV) Receptor-27.8 kDa and Its Expression Kinetics Induced by the Viral Infection in Turbot (Scophthalmus maximus) |
title_fullStr |
Tissue Localization of Lymphocystis Disease Virus (LCDV) Receptor-27.8 kDa and Its Expression Kinetics Induced by the Viral Infection in Turbot (Scophthalmus maximus) |
title_full_unstemmed |
Tissue Localization of Lymphocystis Disease Virus (LCDV) Receptor-27.8 kDa and Its Expression Kinetics Induced by the Viral Infection in Turbot (Scophthalmus maximus) |
title_sort |
tissue localization of lymphocystis disease virus (lcdv) receptor-27.8 kda and its expression kinetics induced by the viral infection in turbot (scophthalmus maximus) |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2015 |
url |
https://doi.org/10.3390/ijms161125974 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_source |
International Journal of Molecular Sciences Volume 16 Issue 11 Pages 26506-26519 |
op_relation |
Biochemistry, Molecular and Cellular Biology |
op_rights |
http://creativecommons.org/licenses/by/3.0/ |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3390/ijms161125974 |
container_title |
International Journal of Molecular Sciences |
container_volume |
16 |
container_issue |
11 |
container_start_page |
26506 |
op_container_end_page |
26519 |
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1766188852557381632 |