Membrane-Based Inverse Transition Cycling: An Improved Means for Purifying Plant-Derived Recombinant Protein-Elastin-Like Polypeptide Fusions
Elastin-like peptide (ELP) was fused to two different avian flu H5N1 antigens and expressed in transgenic tobacco plants. The presence of the ELP tag enhanced the accumulation of the heterologous proteins in the tobacco leaves. An effective membrane-based Inverse Transition Cycling was developed to...
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Molecular Diversity Preservation International
2011
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ftmdpi:oai:mdpi.com:/1422-0067/12/5/2808/ 2023-08-20T04:05:22+02:00 Membrane-Based Inverse Transition Cycling: An Improved Means for Purifying Plant-Derived Recombinant Protein-Elastin-Like Polypeptide Fusions Hoang Trong Phan Udo Conrad agris 2011-04-29 application/pdf https://doi.org/10.3390/ijms12052808 EN eng Molecular Diversity Preservation International Bioactives and Nutraceuticals https://dx.doi.org/10.3390/ijms12052808 https://creativecommons.org/licenses/by/3.0/ International Journal of Molecular Sciences; Volume 12; Issue 5; Pages: 2808-2821 transgenic plants avian influenza H5N1 nanobody ELPylation Inverse Transition Cycling Text 2011 ftmdpi https://doi.org/10.3390/ijms12052808 2023-07-31T20:26:28Z Elastin-like peptide (ELP) was fused to two different avian flu H5N1 antigens and expressed in transgenic tobacco plants. The presence of the ELP tag enhanced the accumulation of the heterologous proteins in the tobacco leaves. An effective membrane-based Inverse Transition Cycling was developed to recover the ELPylated antigens and antibodies from plant material. The functionality of both the ELPylated neuraminidase and an ELPylated nanobody was demonstrated. Text Avian flu MDPI Open Access Publishing International Journal of Molecular Sciences 12 5 2808 2821 |
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Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
transgenic plants avian influenza H5N1 nanobody ELPylation Inverse Transition Cycling |
spellingShingle |
transgenic plants avian influenza H5N1 nanobody ELPylation Inverse Transition Cycling Hoang Trong Phan Udo Conrad Membrane-Based Inverse Transition Cycling: An Improved Means for Purifying Plant-Derived Recombinant Protein-Elastin-Like Polypeptide Fusions |
topic_facet |
transgenic plants avian influenza H5N1 nanobody ELPylation Inverse Transition Cycling |
description |
Elastin-like peptide (ELP) was fused to two different avian flu H5N1 antigens and expressed in transgenic tobacco plants. The presence of the ELP tag enhanced the accumulation of the heterologous proteins in the tobacco leaves. An effective membrane-based Inverse Transition Cycling was developed to recover the ELPylated antigens and antibodies from plant material. The functionality of both the ELPylated neuraminidase and an ELPylated nanobody was demonstrated. |
format |
Text |
author |
Hoang Trong Phan Udo Conrad |
author_facet |
Hoang Trong Phan Udo Conrad |
author_sort |
Hoang Trong Phan |
title |
Membrane-Based Inverse Transition Cycling: An Improved Means for Purifying Plant-Derived Recombinant Protein-Elastin-Like Polypeptide Fusions |
title_short |
Membrane-Based Inverse Transition Cycling: An Improved Means for Purifying Plant-Derived Recombinant Protein-Elastin-Like Polypeptide Fusions |
title_full |
Membrane-Based Inverse Transition Cycling: An Improved Means for Purifying Plant-Derived Recombinant Protein-Elastin-Like Polypeptide Fusions |
title_fullStr |
Membrane-Based Inverse Transition Cycling: An Improved Means for Purifying Plant-Derived Recombinant Protein-Elastin-Like Polypeptide Fusions |
title_full_unstemmed |
Membrane-Based Inverse Transition Cycling: An Improved Means for Purifying Plant-Derived Recombinant Protein-Elastin-Like Polypeptide Fusions |
title_sort |
membrane-based inverse transition cycling: an improved means for purifying plant-derived recombinant protein-elastin-like polypeptide fusions |
publisher |
Molecular Diversity Preservation International |
publishDate |
2011 |
url |
https://doi.org/10.3390/ijms12052808 |
op_coverage |
agris |
genre |
Avian flu |
genre_facet |
Avian flu |
op_source |
International Journal of Molecular Sciences; Volume 12; Issue 5; Pages: 2808-2821 |
op_relation |
Bioactives and Nutraceuticals https://dx.doi.org/10.3390/ijms12052808 |
op_rights |
https://creativecommons.org/licenses/by/3.0/ |
op_doi |
https://doi.org/10.3390/ijms12052808 |
container_title |
International Journal of Molecular Sciences |
container_volume |
12 |
container_issue |
5 |
container_start_page |
2808 |
op_container_end_page |
2821 |
_version_ |
1774715884081774592 |