DES-Based Biocatalysis as a Green Alternative for the l-menthyl Ester Production Based on l-menthol Acylation
The deep eutectic solvent (DES)-based biocatalysis of l-menthol acylation was designed for the production of fatty acid l-menthyl ester (FME) using fatty acid methyl ester (FAME). The biocatalytic reaction was assisted by a lipase enzyme in the DES reaction medium. ւՒ-menthol and fatty acids (e.g.,...
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ftmdpi:oai:mdpi.com:/1420-3049/27/16/5273/ 2023-08-20T04:01:36+02:00 DES-Based Biocatalysis as a Green Alternative for the l-menthyl Ester Production Based on l-menthol Acylation Sabina Ion Florentina Olănescu Florina Teodorescu Robert Tincu Daniela Gheorghe Vasile I. Pârvulescu Mădălina Tudorache agris 2022-08-18 application/pdf https://doi.org/10.3390/molecules27165273 EN eng Multidisciplinary Digital Publishing Institute Organic Chemistry https://dx.doi.org/10.3390/molecules27165273 https://creativecommons.org/licenses/by/4.0/ Molecules; Volume 27; Issue 16; Pages: 5273 biocatalysis DES l -menthol fatty acid l -menthyl ester (FME) immobilized lipase Text 2022 ftmdpi https://doi.org/10.3390/molecules27165273 2023-08-01T06:07:29Z The deep eutectic solvent (DES)-based biocatalysis of l-menthol acylation was designed for the production of fatty acid l-menthyl ester (FME) using fatty acid methyl ester (FAME). The biocatalytic reaction was assisted by a lipase enzyme in the DES reaction medium. ւՒ-menthol and fatty acids (e.g., CA—caprylic acid; OA—oleic acid; LiA—linoleic acid; and LnA—linolenic acid) were combined in the binary mixture of DES. In this way, the DES provided a nonpolar environment for requested homogeneity of a biocatalytic system with reduced impact on the environment. The screening of lipase enzyme demonstrated better performance of immobilized lipase compared with powdered lipase. The performance of the biocatalytic system was evaluated for different DES compositions (type and concentration of the acid component). l-menthol:CA = 73:27 molar ratio allowed it to reach a maximum conversion of 95% methyl lauric ester (MLE) using a NV (Candida antarctica lipase B immobilized on acrylic resin) lipase biocatalyst. The recyclability of biocatalysts under optimum conditions of the system was also evaluated (more than 80% recovered biocatalytic activity was achieved for the tested biocatalysts after five reaction cycles). DES mixtures were characterized based on differential scanning calorimetry (DSC) and refractive index analysis. Text Antarc* Antarctica MDPI Open Access Publishing Molecules 27 16 5273 |
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MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
biocatalysis DES l -menthol fatty acid l -menthyl ester (FME) immobilized lipase |
spellingShingle |
biocatalysis DES l -menthol fatty acid l -menthyl ester (FME) immobilized lipase Sabina Ion Florentina Olănescu Florina Teodorescu Robert Tincu Daniela Gheorghe Vasile I. Pârvulescu Mădălina Tudorache DES-Based Biocatalysis as a Green Alternative for the l-menthyl Ester Production Based on l-menthol Acylation |
topic_facet |
biocatalysis DES l -menthol fatty acid l -menthyl ester (FME) immobilized lipase |
description |
The deep eutectic solvent (DES)-based biocatalysis of l-menthol acylation was designed for the production of fatty acid l-menthyl ester (FME) using fatty acid methyl ester (FAME). The biocatalytic reaction was assisted by a lipase enzyme in the DES reaction medium. ւՒ-menthol and fatty acids (e.g., CA—caprylic acid; OA—oleic acid; LiA—linoleic acid; and LnA—linolenic acid) were combined in the binary mixture of DES. In this way, the DES provided a nonpolar environment for requested homogeneity of a biocatalytic system with reduced impact on the environment. The screening of lipase enzyme demonstrated better performance of immobilized lipase compared with powdered lipase. The performance of the biocatalytic system was evaluated for different DES compositions (type and concentration of the acid component). l-menthol:CA = 73:27 molar ratio allowed it to reach a maximum conversion of 95% methyl lauric ester (MLE) using a NV (Candida antarctica lipase B immobilized on acrylic resin) lipase biocatalyst. The recyclability of biocatalysts under optimum conditions of the system was also evaluated (more than 80% recovered biocatalytic activity was achieved for the tested biocatalysts after five reaction cycles). DES mixtures were characterized based on differential scanning calorimetry (DSC) and refractive index analysis. |
format |
Text |
author |
Sabina Ion Florentina Olănescu Florina Teodorescu Robert Tincu Daniela Gheorghe Vasile I. Pârvulescu Mădălina Tudorache |
author_facet |
Sabina Ion Florentina Olănescu Florina Teodorescu Robert Tincu Daniela Gheorghe Vasile I. Pârvulescu Mădălina Tudorache |
author_sort |
Sabina Ion |
title |
DES-Based Biocatalysis as a Green Alternative for the l-menthyl Ester Production Based on l-menthol Acylation |
title_short |
DES-Based Biocatalysis as a Green Alternative for the l-menthyl Ester Production Based on l-menthol Acylation |
title_full |
DES-Based Biocatalysis as a Green Alternative for the l-menthyl Ester Production Based on l-menthol Acylation |
title_fullStr |
DES-Based Biocatalysis as a Green Alternative for the l-menthyl Ester Production Based on l-menthol Acylation |
title_full_unstemmed |
DES-Based Biocatalysis as a Green Alternative for the l-menthyl Ester Production Based on l-menthol Acylation |
title_sort |
des-based biocatalysis as a green alternative for the l-menthyl ester production based on l-menthol acylation |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2022 |
url |
https://doi.org/10.3390/molecules27165273 |
op_coverage |
agris |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Molecules; Volume 27; Issue 16; Pages: 5273 |
op_relation |
Organic Chemistry https://dx.doi.org/10.3390/molecules27165273 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/molecules27165273 |
container_title |
Molecules |
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27 |
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16 |
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5273 |
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1774724853101756416 |