Peptide Biomarkers Discovery for Seven Species of Deer Antler Using LC-MS/MS and Label-Free Approach
Deer antler is a globally widely used precious natural medicine and the material of deer horn gelatin. However, identification of deer antler species based on traditional approaches are problematic because of their similarity in appearance and physical-chemical properties. In this study, we performe...
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ftmdpi:oai:mdpi.com:/1420-3049/27/15/4756/ 2023-08-20T03:59:23+02:00 Peptide Biomarkers Discovery for Seven Species of Deer Antler Using LC-MS/MS and Label-Free Approach Fei Xue Bing Wang Dong-Xiao Guo Yang Jiao Xue Yin Wei-Liang Cui Qian-Qian Zhou Feng-Rui Yu Yong-Qiang Lin agris 2022-07-25 application/pdf https://doi.org/10.3390/molecules27154756 EN eng Multidisciplinary Digital Publishing Institute Applied Chemistry https://dx.doi.org/10.3390/molecules27154756 https://creativecommons.org/licenses/by/4.0/ Molecules; Volume 27; Issue 15; Pages: 4756 adult beta-globin deer antler peptide biomarkers deer species identification label-free approach Text 2022 ftmdpi https://doi.org/10.3390/molecules27154756 2023-08-01T05:50:12Z Deer antler is a globally widely used precious natural medicine and the material of deer horn gelatin. However, identification of deer antler species based on traditional approaches are problematic because of their similarity in appearance and physical-chemical properties. In this study, we performed a comprehensive antler peptidome analysis using a label-free approach: nano LC-Orbitrap MS was applied to discover peptide biomarkers in deer adult beta-globin (HBBA), and HPLC-Triple Quadrupole MS was used to verify their specificity. Nineteen peptide biomarkers were found, on which foundation a strategy for antlers and a strategy for antler mixtures such as flakes or powder are provided to identify seven species of deer antler including Eurasian elk (Alces alces), reindeer (Rangifer tarandus), white-tailed deer (Odocoileus viginianus), white-lipped deer (Przewalskium albirostris), fallow deer (Dama dama), sika deer (Cervus nippon), and red deer (Cervus elaphus) simultaneously. It is worth noting that our search found that the HBBA gene of sika deer, red deer, and North American wapiti (Cervus canadensis) in China may have undergone severe genetic drifts. Text Alces alces Rangifer tarandus MDPI Open Access Publishing Molecules 27 15 4756 |
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English |
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adult beta-globin deer antler peptide biomarkers deer species identification label-free approach |
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adult beta-globin deer antler peptide biomarkers deer species identification label-free approach Fei Xue Bing Wang Dong-Xiao Guo Yang Jiao Xue Yin Wei-Liang Cui Qian-Qian Zhou Feng-Rui Yu Yong-Qiang Lin Peptide Biomarkers Discovery for Seven Species of Deer Antler Using LC-MS/MS and Label-Free Approach |
topic_facet |
adult beta-globin deer antler peptide biomarkers deer species identification label-free approach |
description |
Deer antler is a globally widely used precious natural medicine and the material of deer horn gelatin. However, identification of deer antler species based on traditional approaches are problematic because of their similarity in appearance and physical-chemical properties. In this study, we performed a comprehensive antler peptidome analysis using a label-free approach: nano LC-Orbitrap MS was applied to discover peptide biomarkers in deer adult beta-globin (HBBA), and HPLC-Triple Quadrupole MS was used to verify their specificity. Nineteen peptide biomarkers were found, on which foundation a strategy for antlers and a strategy for antler mixtures such as flakes or powder are provided to identify seven species of deer antler including Eurasian elk (Alces alces), reindeer (Rangifer tarandus), white-tailed deer (Odocoileus viginianus), white-lipped deer (Przewalskium albirostris), fallow deer (Dama dama), sika deer (Cervus nippon), and red deer (Cervus elaphus) simultaneously. It is worth noting that our search found that the HBBA gene of sika deer, red deer, and North American wapiti (Cervus canadensis) in China may have undergone severe genetic drifts. |
format |
Text |
author |
Fei Xue Bing Wang Dong-Xiao Guo Yang Jiao Xue Yin Wei-Liang Cui Qian-Qian Zhou Feng-Rui Yu Yong-Qiang Lin |
author_facet |
Fei Xue Bing Wang Dong-Xiao Guo Yang Jiao Xue Yin Wei-Liang Cui Qian-Qian Zhou Feng-Rui Yu Yong-Qiang Lin |
author_sort |
Fei Xue |
title |
Peptide Biomarkers Discovery for Seven Species of Deer Antler Using LC-MS/MS and Label-Free Approach |
title_short |
Peptide Biomarkers Discovery for Seven Species of Deer Antler Using LC-MS/MS and Label-Free Approach |
title_full |
Peptide Biomarkers Discovery for Seven Species of Deer Antler Using LC-MS/MS and Label-Free Approach |
title_fullStr |
Peptide Biomarkers Discovery for Seven Species of Deer Antler Using LC-MS/MS and Label-Free Approach |
title_full_unstemmed |
Peptide Biomarkers Discovery for Seven Species of Deer Antler Using LC-MS/MS and Label-Free Approach |
title_sort |
peptide biomarkers discovery for seven species of deer antler using lc-ms/ms and label-free approach |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2022 |
url |
https://doi.org/10.3390/molecules27154756 |
op_coverage |
agris |
genre |
Alces alces Rangifer tarandus |
genre_facet |
Alces alces Rangifer tarandus |
op_source |
Molecules; Volume 27; Issue 15; Pages: 4756 |
op_relation |
Applied Chemistry https://dx.doi.org/10.3390/molecules27154756 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/molecules27154756 |
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Molecules |
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27 |
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15 |
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4756 |
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1774712628277411840 |