Neurorescue Effects of Frondoside A and Ginsenoside Rg3 in C. elegans Model of Parkinson’s Disease
Parkinson’s disease (PD) is a currently incurable neurodegenerative disorder characterized by the loss of dopaminergic (DAergic) neurons in the substantia nigra pars compacta and α-synuclein aggregation. Accumulated evidence indicates that the saponins, especially from ginseng, have neuroprotective...
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ftmdpi:oai:mdpi.com:/1420-3049/26/16/4843/ 2023-08-20T04:06:06+02:00 Neurorescue Effects of Frondoside A and Ginsenoside Rg3 in C. elegans Model of Parkinson’s Disease Pawanrat Chalorak Tanatcha Sanguanphun Tanapol Limboonreung Krai Meemon agris 2021-08-10 application/pdf https://doi.org/10.3390/molecules26164843 EN eng Multidisciplinary Digital Publishing Institute Natural Products Chemistry https://dx.doi.org/10.3390/molecules26164843 https://creativecommons.org/licenses/by/4.0/ Molecules; Volume 26; Issue 16; Pages: 4843 Parkinson’s disease dopaminergic neurons neurodegeneration α-Synuclein frondoside A ginsenoside Rg3 Caenorhabditis elegans Text 2021 ftmdpi https://doi.org/10.3390/molecules26164843 2023-08-01T02:24:25Z Parkinson’s disease (PD) is a currently incurable neurodegenerative disorder characterized by the loss of dopaminergic (DAergic) neurons in the substantia nigra pars compacta and α-synuclein aggregation. Accumulated evidence indicates that the saponins, especially from ginseng, have neuroprotective effects against neurodegenerative disorders. Interestingly, saponin can also be found in marine organisms such as the sea cucumber, but little is known about its effect in neurodegenerative disease, including PD. In this study, we investigated the anti-Parkinson effects of frondoside A (FA) from Cucumaria frondosa and ginsenoside Rg3 (Rg3) from Panax notoginseng in C. elegans PD model. Both saponins were tested for toxicity and optimal concentration by food clearance assay and used to treat 6-OHDA-induced BZ555 and transgenic α-synuclein NL5901 strains in C. elegans. Treatment with FA and Rg3 significantly attenuated DAergic neurodegeneration induced by 6-OHDA in BZ555 strain, improved basal slowing rate, and prolonged lifespan in the 6-OHDA-induced wild-type strain with downregulation of the apoptosis mediators, egl-1 and ced-3, and upregulation of sod-3 and cat-2. Interestingly, only FA reduced α-synuclein aggregation, rescued lifespan in NL5901, and upregulated the protein degradation regulators, including ubh-4, hsf-1, hsp-16.1 and hsp-16.2. This study indicates that both FA and Rg3 possess beneficial effects in rescuing DAergic neurodegeneration in the 6-OHDA-induced C. elegans model through suppressing apoptosis mediators and stimulating antioxidant enzymes. In addition, FA could attenuate α-synuclein aggregation through the protein degradation process. Text Cucumaria frondosa MDPI Open Access Publishing Molecules 26 16 4843 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
Parkinson’s disease dopaminergic neurons neurodegeneration α-Synuclein frondoside A ginsenoside Rg3 Caenorhabditis elegans |
spellingShingle |
Parkinson’s disease dopaminergic neurons neurodegeneration α-Synuclein frondoside A ginsenoside Rg3 Caenorhabditis elegans Pawanrat Chalorak Tanatcha Sanguanphun Tanapol Limboonreung Krai Meemon Neurorescue Effects of Frondoside A and Ginsenoside Rg3 in C. elegans Model of Parkinson’s Disease |
topic_facet |
Parkinson’s disease dopaminergic neurons neurodegeneration α-Synuclein frondoside A ginsenoside Rg3 Caenorhabditis elegans |
description |
Parkinson’s disease (PD) is a currently incurable neurodegenerative disorder characterized by the loss of dopaminergic (DAergic) neurons in the substantia nigra pars compacta and α-synuclein aggregation. Accumulated evidence indicates that the saponins, especially from ginseng, have neuroprotective effects against neurodegenerative disorders. Interestingly, saponin can also be found in marine organisms such as the sea cucumber, but little is known about its effect in neurodegenerative disease, including PD. In this study, we investigated the anti-Parkinson effects of frondoside A (FA) from Cucumaria frondosa and ginsenoside Rg3 (Rg3) from Panax notoginseng in C. elegans PD model. Both saponins were tested for toxicity and optimal concentration by food clearance assay and used to treat 6-OHDA-induced BZ555 and transgenic α-synuclein NL5901 strains in C. elegans. Treatment with FA and Rg3 significantly attenuated DAergic neurodegeneration induced by 6-OHDA in BZ555 strain, improved basal slowing rate, and prolonged lifespan in the 6-OHDA-induced wild-type strain with downregulation of the apoptosis mediators, egl-1 and ced-3, and upregulation of sod-3 and cat-2. Interestingly, only FA reduced α-synuclein aggregation, rescued lifespan in NL5901, and upregulated the protein degradation regulators, including ubh-4, hsf-1, hsp-16.1 and hsp-16.2. This study indicates that both FA and Rg3 possess beneficial effects in rescuing DAergic neurodegeneration in the 6-OHDA-induced C. elegans model through suppressing apoptosis mediators and stimulating antioxidant enzymes. In addition, FA could attenuate α-synuclein aggregation through the protein degradation process. |
format |
Text |
author |
Pawanrat Chalorak Tanatcha Sanguanphun Tanapol Limboonreung Krai Meemon |
author_facet |
Pawanrat Chalorak Tanatcha Sanguanphun Tanapol Limboonreung Krai Meemon |
author_sort |
Pawanrat Chalorak |
title |
Neurorescue Effects of Frondoside A and Ginsenoside Rg3 in C. elegans Model of Parkinson’s Disease |
title_short |
Neurorescue Effects of Frondoside A and Ginsenoside Rg3 in C. elegans Model of Parkinson’s Disease |
title_full |
Neurorescue Effects of Frondoside A and Ginsenoside Rg3 in C. elegans Model of Parkinson’s Disease |
title_fullStr |
Neurorescue Effects of Frondoside A and Ginsenoside Rg3 in C. elegans Model of Parkinson’s Disease |
title_full_unstemmed |
Neurorescue Effects of Frondoside A and Ginsenoside Rg3 in C. elegans Model of Parkinson’s Disease |
title_sort |
neurorescue effects of frondoside a and ginsenoside rg3 in c. elegans model of parkinson’s disease |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2021 |
url |
https://doi.org/10.3390/molecules26164843 |
op_coverage |
agris |
genre |
Cucumaria frondosa |
genre_facet |
Cucumaria frondosa |
op_source |
Molecules; Volume 26; Issue 16; Pages: 4843 |
op_relation |
Natural Products Chemistry https://dx.doi.org/10.3390/molecules26164843 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/molecules26164843 |
container_title |
Molecules |
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26 |
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16 |
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4843 |
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1774717022050975744 |