Assessment of Astaxanthin Accumulation in Hepatocytes of Atlantic Salmon Fed Different Diets Using NMR Spectroscopy
This study aimed to assess the astaxanthin (Ax) accumulation in hepatocytes isolated from farmed Atlantic salmon fed different diets (rich marine, poor, poor with marine phospholipids (MPL) and poor with docosahexaenoic acid (DHA)). Nuclear magnetic resonance (NMR) spectroscopy was used for the Ax d...
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ftmdpi:oai:mdpi.com:/1420-3049/25/8/1769/ 2023-08-20T04:05:14+02:00 Assessment of Astaxanthin Accumulation in Hepatocytes of Atlantic Salmon Fed Different Diets Using NMR Spectroscopy Elena Shumilina Alessandra Ciampa Trine Ytrestøyl Alexander Dikiy agris 2020-04-12 application/pdf https://doi.org/10.3390/molecules25081769 EN eng Multidisciplinary Digital Publishing Institute https://dx.doi.org/10.3390/molecules25081769 https://creativecommons.org/licenses/by/4.0/ Molecules; Volume 25; Issue 8; Pages: 1769 carotenoid astaxanthin Atlantic salmon fish diet fish feed plant proteins DHA hepatocytes NMR Text 2020 ftmdpi https://doi.org/10.3390/molecules25081769 2023-07-31T23:21:51Z This study aimed to assess the astaxanthin (Ax) accumulation in hepatocytes isolated from farmed Atlantic salmon fed different diets (rich marine, poor, poor with marine phospholipids (MPL) and poor with docosahexaenoic acid (DHA)). Nuclear magnetic resonance (NMR) spectroscopy was used for the Ax detection and quantification. The use of the 13C-enriched Ax allowed the assessment of short-time Ax metabolism. The substitution of fish oil and meal in fish feed on plant analogs and the addition of MPL caused further catabolism and decrease of Ax accumulation in hepatocytes from 17 to about 6 mg/kg or to almost zero in the case of DHA addition. Signals assignment of the native and 13C-enriched astaxanthin in acetone were performed using 1D and 2D NMR spectra. Text Atlantic salmon MDPI Open Access Publishing Molecules 25 8 1769 |
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Open Polar |
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MDPI Open Access Publishing |
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ftmdpi |
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English |
topic |
carotenoid astaxanthin Atlantic salmon fish diet fish feed plant proteins DHA hepatocytes NMR |
spellingShingle |
carotenoid astaxanthin Atlantic salmon fish diet fish feed plant proteins DHA hepatocytes NMR Elena Shumilina Alessandra Ciampa Trine Ytrestøyl Alexander Dikiy Assessment of Astaxanthin Accumulation in Hepatocytes of Atlantic Salmon Fed Different Diets Using NMR Spectroscopy |
topic_facet |
carotenoid astaxanthin Atlantic salmon fish diet fish feed plant proteins DHA hepatocytes NMR |
description |
This study aimed to assess the astaxanthin (Ax) accumulation in hepatocytes isolated from farmed Atlantic salmon fed different diets (rich marine, poor, poor with marine phospholipids (MPL) and poor with docosahexaenoic acid (DHA)). Nuclear magnetic resonance (NMR) spectroscopy was used for the Ax detection and quantification. The use of the 13C-enriched Ax allowed the assessment of short-time Ax metabolism. The substitution of fish oil and meal in fish feed on plant analogs and the addition of MPL caused further catabolism and decrease of Ax accumulation in hepatocytes from 17 to about 6 mg/kg or to almost zero in the case of DHA addition. Signals assignment of the native and 13C-enriched astaxanthin in acetone were performed using 1D and 2D NMR spectra. |
format |
Text |
author |
Elena Shumilina Alessandra Ciampa Trine Ytrestøyl Alexander Dikiy |
author_facet |
Elena Shumilina Alessandra Ciampa Trine Ytrestøyl Alexander Dikiy |
author_sort |
Elena Shumilina |
title |
Assessment of Astaxanthin Accumulation in Hepatocytes of Atlantic Salmon Fed Different Diets Using NMR Spectroscopy |
title_short |
Assessment of Astaxanthin Accumulation in Hepatocytes of Atlantic Salmon Fed Different Diets Using NMR Spectroscopy |
title_full |
Assessment of Astaxanthin Accumulation in Hepatocytes of Atlantic Salmon Fed Different Diets Using NMR Spectroscopy |
title_fullStr |
Assessment of Astaxanthin Accumulation in Hepatocytes of Atlantic Salmon Fed Different Diets Using NMR Spectroscopy |
title_full_unstemmed |
Assessment of Astaxanthin Accumulation in Hepatocytes of Atlantic Salmon Fed Different Diets Using NMR Spectroscopy |
title_sort |
assessment of astaxanthin accumulation in hepatocytes of atlantic salmon fed different diets using nmr spectroscopy |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2020 |
url |
https://doi.org/10.3390/molecules25081769 |
op_coverage |
agris |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Molecules; Volume 25; Issue 8; Pages: 1769 |
op_relation |
https://dx.doi.org/10.3390/molecules25081769 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/molecules25081769 |
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Molecules |
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25 |
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8 |
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1769 |
_version_ |
1774715726008942592 |