New Arctic Bacterial Isolates with Relevant Enzymatic Potential
Fragments of wood drifting in the vicinity of Spitzbergen were used for the isolation of microorganisms, carried out using atypical carbon sources: colloidal chitin, cellulose and carboxymethylcellulose, xylan, casein, tributrin and olive oil. Purified cultures were subjected to a three-step identif...
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ftmdpi:oai:mdpi.com:/1420-3049/25/17/3930/ 2023-08-20T04:04:33+02:00 New Arctic Bacterial Isolates with Relevant Enzymatic Potential Michał Piegza Wojciech Łaba Miroslava Kačániová agris 2020-08-28 application/pdf https://doi.org/10.3390/molecules25173930 EN eng Multidisciplinary Digital Publishing Institute https://dx.doi.org/10.3390/molecules25173930 https://creativecommons.org/licenses/by/4.0/ Molecules; Volume 25; Issue 17; Pages: 3930 MALDI-TOF MS Biotyper molecular fluorescence hydrolytic enzymes Text 2020 ftmdpi https://doi.org/10.3390/molecules25173930 2023-07-31T23:59:48Z Fragments of wood drifting in the vicinity of Spitzbergen were used for the isolation of microorganisms, carried out using atypical carbon sources: colloidal chitin, cellulose and carboxymethylcellulose, xylan, casein, tributrin and olive oil. Purified cultures were subjected to a three-step identification: with classical methods, using MALDI-TOF MS Biotyper whole-cell protein fingerprinting, and molecular analysis of 16S rDNA. Subsequently, a preliminary assessment of the enzymatic potential of isolates was carried out. As a result, cellulolytic activity was observed in more than 50% of the bacterial strains, exhibiting activity of 0.30–0.40 U/mL. Over 53% of the isolates demonstrated xylanolytic activity, of which the highest reached from 0.40 to 0.90 U. Polygalacturonase activity of 0.003–1.6 was also demonstrated in half of the bacterial strains studied. Proteolytic activity of isolates did not exceed 0.3 U. An important highlight was the ability of fluorescent dye production by certain strains, grown on skim milk-agar, but also on pure meat extract. Text Arctic Spitzbergen MDPI Open Access Publishing Arctic Molecules 25 17 3930 |
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Open Polar |
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MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
MALDI-TOF MS Biotyper molecular fluorescence hydrolytic enzymes |
spellingShingle |
MALDI-TOF MS Biotyper molecular fluorescence hydrolytic enzymes Michał Piegza Wojciech Łaba Miroslava Kačániová New Arctic Bacterial Isolates with Relevant Enzymatic Potential |
topic_facet |
MALDI-TOF MS Biotyper molecular fluorescence hydrolytic enzymes |
description |
Fragments of wood drifting in the vicinity of Spitzbergen were used for the isolation of microorganisms, carried out using atypical carbon sources: colloidal chitin, cellulose and carboxymethylcellulose, xylan, casein, tributrin and olive oil. Purified cultures were subjected to a three-step identification: with classical methods, using MALDI-TOF MS Biotyper whole-cell protein fingerprinting, and molecular analysis of 16S rDNA. Subsequently, a preliminary assessment of the enzymatic potential of isolates was carried out. As a result, cellulolytic activity was observed in more than 50% of the bacterial strains, exhibiting activity of 0.30–0.40 U/mL. Over 53% of the isolates demonstrated xylanolytic activity, of which the highest reached from 0.40 to 0.90 U. Polygalacturonase activity of 0.003–1.6 was also demonstrated in half of the bacterial strains studied. Proteolytic activity of isolates did not exceed 0.3 U. An important highlight was the ability of fluorescent dye production by certain strains, grown on skim milk-agar, but also on pure meat extract. |
format |
Text |
author |
Michał Piegza Wojciech Łaba Miroslava Kačániová |
author_facet |
Michał Piegza Wojciech Łaba Miroslava Kačániová |
author_sort |
Michał Piegza |
title |
New Arctic Bacterial Isolates with Relevant Enzymatic Potential |
title_short |
New Arctic Bacterial Isolates with Relevant Enzymatic Potential |
title_full |
New Arctic Bacterial Isolates with Relevant Enzymatic Potential |
title_fullStr |
New Arctic Bacterial Isolates with Relevant Enzymatic Potential |
title_full_unstemmed |
New Arctic Bacterial Isolates with Relevant Enzymatic Potential |
title_sort |
new arctic bacterial isolates with relevant enzymatic potential |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2020 |
url |
https://doi.org/10.3390/molecules25173930 |
op_coverage |
agris |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Spitzbergen |
genre_facet |
Arctic Spitzbergen |
op_source |
Molecules; Volume 25; Issue 17; Pages: 3930 |
op_relation |
https://dx.doi.org/10.3390/molecules25173930 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/molecules25173930 |
container_title |
Molecules |
container_volume |
25 |
container_issue |
17 |
container_start_page |
3930 |
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1774714926723497984 |