Biosurfactant Production and Growth Kinetics Studies of the Waste Canola Oil-Degrading Bacterium Rhodococcus erythropolis AQ5-07 from Antarctica
With the progressive increase in human activities in the Antarctic region, the possibility of domestic oil spillage also increases. Developing means for the removal of oils, such as canola oil, from the environment and waste “grey” water using biological approaches is therefore desirable, since the...
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ftmdpi:oai:mdpi.com:/1420-3049/25/17/3878/ 2023-08-20T04:00:04+02:00 Biosurfactant Production and Growth Kinetics Studies of the Waste Canola Oil-Degrading Bacterium Rhodococcus erythropolis AQ5-07 from Antarctica Salihu Ibrahim Khalilah Abdul Khalil Khadijah Nabilah Mohd Zahri Claudio Gomez-Fuentes Peter Convey Azham Zulkharnain Suriana Sabri Siti Aisyah Alias Gerardo González-Rocha Siti Aqlima Ahmad agris 2020-08-26 application/pdf https://doi.org/10.3390/molecules25173878 EN eng Multidisciplinary Digital Publishing Institute https://dx.doi.org/10.3390/molecules25173878 https://creativecommons.org/licenses/by/4.0/ Molecules; Volume 25; Issue 17; Pages: 3878 Antarctica biosurfactants canola oil kinetics haldane modelling Rhodococcus erythropolis AQ5-07 Text 2020 ftmdpi https://doi.org/10.3390/molecules25173878 2023-07-31T23:58:59Z With the progressive increase in human activities in the Antarctic region, the possibility of domestic oil spillage also increases. Developing means for the removal of oils, such as canola oil, from the environment and waste “grey” water using biological approaches is therefore desirable, since the thermal process of oil degradation is expensive and ineffective. Thus, in this study an indigenous cold-adapted Antarctic soil bacterium, Rhodococcus erythropolis strain AQ5-07, was screened for biosurfactant production ability using the multiple approaches of blood haemolysis, surface tension, emulsification index, oil spreading, drop collapse and “MATH” assay for cellular hydrophobicity. The growth kinetics of the bacterium containing different canola oil concentration was studied. The strain showed β-haemolysis on blood agar with a high emulsification index and low surface tension value of 91.5% and 25.14 mN/m, respectively. Of the models tested, the Haldane model provided the best description of the growth kinetics, although several models were similar in performance. Parameters obtained from the modelling were the maximum specific growth rate (qmax), concentration of substrate at the half maximum specific growth rate, Ks% (v/v) and the inhibition constant Ki% (v/v), with values of 0.142 h−1, 7.743% (v/v) and 0.399% (v/v), respectively. These biological coefficients are useful in predicting growth conditions for batch studies, and also relevant to “in field” bioremediation strategies where the concentration of oil might need to be diluted to non-toxic levels prior to remediation. Biosurfactants can also have application in enhanced oil recovery (EOR) under different environmental conditions. Text Antarc* Antarctic Antarctica MDPI Open Access Publishing Antarctic The Antarctic Molecules 25 17 3878 |
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MDPI Open Access Publishing |
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ftmdpi |
language |
English |
topic |
Antarctica biosurfactants canola oil kinetics haldane modelling Rhodococcus erythropolis AQ5-07 |
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Antarctica biosurfactants canola oil kinetics haldane modelling Rhodococcus erythropolis AQ5-07 Salihu Ibrahim Khalilah Abdul Khalil Khadijah Nabilah Mohd Zahri Claudio Gomez-Fuentes Peter Convey Azham Zulkharnain Suriana Sabri Siti Aisyah Alias Gerardo González-Rocha Siti Aqlima Ahmad Biosurfactant Production and Growth Kinetics Studies of the Waste Canola Oil-Degrading Bacterium Rhodococcus erythropolis AQ5-07 from Antarctica |
topic_facet |
Antarctica biosurfactants canola oil kinetics haldane modelling Rhodococcus erythropolis AQ5-07 |
description |
With the progressive increase in human activities in the Antarctic region, the possibility of domestic oil spillage also increases. Developing means for the removal of oils, such as canola oil, from the environment and waste “grey” water using biological approaches is therefore desirable, since the thermal process of oil degradation is expensive and ineffective. Thus, in this study an indigenous cold-adapted Antarctic soil bacterium, Rhodococcus erythropolis strain AQ5-07, was screened for biosurfactant production ability using the multiple approaches of blood haemolysis, surface tension, emulsification index, oil spreading, drop collapse and “MATH” assay for cellular hydrophobicity. The growth kinetics of the bacterium containing different canola oil concentration was studied. The strain showed β-haemolysis on blood agar with a high emulsification index and low surface tension value of 91.5% and 25.14 mN/m, respectively. Of the models tested, the Haldane model provided the best description of the growth kinetics, although several models were similar in performance. Parameters obtained from the modelling were the maximum specific growth rate (qmax), concentration of substrate at the half maximum specific growth rate, Ks% (v/v) and the inhibition constant Ki% (v/v), with values of 0.142 h−1, 7.743% (v/v) and 0.399% (v/v), respectively. These biological coefficients are useful in predicting growth conditions for batch studies, and also relevant to “in field” bioremediation strategies where the concentration of oil might need to be diluted to non-toxic levels prior to remediation. Biosurfactants can also have application in enhanced oil recovery (EOR) under different environmental conditions. |
format |
Text |
author |
Salihu Ibrahim Khalilah Abdul Khalil Khadijah Nabilah Mohd Zahri Claudio Gomez-Fuentes Peter Convey Azham Zulkharnain Suriana Sabri Siti Aisyah Alias Gerardo González-Rocha Siti Aqlima Ahmad |
author_facet |
Salihu Ibrahim Khalilah Abdul Khalil Khadijah Nabilah Mohd Zahri Claudio Gomez-Fuentes Peter Convey Azham Zulkharnain Suriana Sabri Siti Aisyah Alias Gerardo González-Rocha Siti Aqlima Ahmad |
author_sort |
Salihu Ibrahim |
title |
Biosurfactant Production and Growth Kinetics Studies of the Waste Canola Oil-Degrading Bacterium Rhodococcus erythropolis AQ5-07 from Antarctica |
title_short |
Biosurfactant Production and Growth Kinetics Studies of the Waste Canola Oil-Degrading Bacterium Rhodococcus erythropolis AQ5-07 from Antarctica |
title_full |
Biosurfactant Production and Growth Kinetics Studies of the Waste Canola Oil-Degrading Bacterium Rhodococcus erythropolis AQ5-07 from Antarctica |
title_fullStr |
Biosurfactant Production and Growth Kinetics Studies of the Waste Canola Oil-Degrading Bacterium Rhodococcus erythropolis AQ5-07 from Antarctica |
title_full_unstemmed |
Biosurfactant Production and Growth Kinetics Studies of the Waste Canola Oil-Degrading Bacterium Rhodococcus erythropolis AQ5-07 from Antarctica |
title_sort |
biosurfactant production and growth kinetics studies of the waste canola oil-degrading bacterium rhodococcus erythropolis aq5-07 from antarctica |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2020 |
url |
https://doi.org/10.3390/molecules25173878 |
op_coverage |
agris |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_source |
Molecules; Volume 25; Issue 17; Pages: 3878 |
op_relation |
https://dx.doi.org/10.3390/molecules25173878 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/molecules25173878 |
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Molecules |
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25 |
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17 |
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3878 |
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1774716595977846784 |