Immobilization in Ionogel: A New Way to Improve the Activity and Stability of Candida antarctica Lipase B
New Candida antarctica lipase B derivatives with higher activity than the free enzyme were obtained by occlusion in an organogel of an ionic liquid (ionogel) based on the ionic liquid [Omim][PF6] and polyvinyl chloride. The inclusion of glutaraldehyde as a crosslinker improved the properties of the...
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ftmdpi:oai:mdpi.com:/1420-3049/25/14/3233/ 2023-08-20T04:02:34+02:00 Immobilization in Ionogel: A New Way to Improve the Activity and Stability of Candida antarctica Lipase B Alfonso Escudero Antonia Pérez de los Ríos Carlos Godínez Francisca Tomás Francisco José Hernández-Fernández agris 2020-07-15 application/pdf https://doi.org/10.3390/molecules25143233 EN eng Multidisciplinary Digital Publishing Institute Green Chemistry https://dx.doi.org/10.3390/molecules25143233 https://creativecommons.org/licenses/by/4.0/ Molecules; Volume 25; Issue 14; Pages: 3233 enzymatic immobilization organogels ionogels ionic liquid ester synthesis enzyme green chemistry Text 2020 ftmdpi https://doi.org/10.3390/molecules25143233 2023-07-31T23:46:46Z New Candida antarctica lipase B derivatives with higher activity than the free enzyme were obtained by occlusion in an organogel of an ionic liquid (ionogel) based on the ionic liquid [Omim][PF6] and polyvinyl chloride. The inclusion of glutaraldehyde as a crosslinker improved the properties of the ionogel, allowing the enzymatic derivative to reach 5-fold higher activity than the free enzyme and also allowing it to be reused at 70 °C. The new methodology allows enzymatic derivatives to be designed by changing the ionic liquid, thus providing a suitable microenvironment for the enzyme. The ionic liquid may act on substrates to increase their local concentration, while reducing water activity in the enzyme’s microenvironment. All this allows the activity and selectivity of the enzyme to be improved and greener processes to be developed. The chemical composition and morphology of the ionogel were also studied by scanning electron microscopy–energy dispersive X-ray spectroscopy, finding that porosity, which was related with the chemical composition, was a key factor for the enzyme activity. Text Antarc* Antarctica MDPI Open Access Publishing Molecules 25 14 3233 |
institution |
Open Polar |
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MDPI Open Access Publishing |
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ftmdpi |
language |
English |
topic |
enzymatic immobilization organogels ionogels ionic liquid ester synthesis enzyme green chemistry |
spellingShingle |
enzymatic immobilization organogels ionogels ionic liquid ester synthesis enzyme green chemistry Alfonso Escudero Antonia Pérez de los Ríos Carlos Godínez Francisca Tomás Francisco José Hernández-Fernández Immobilization in Ionogel: A New Way to Improve the Activity and Stability of Candida antarctica Lipase B |
topic_facet |
enzymatic immobilization organogels ionogels ionic liquid ester synthesis enzyme green chemistry |
description |
New Candida antarctica lipase B derivatives with higher activity than the free enzyme were obtained by occlusion in an organogel of an ionic liquid (ionogel) based on the ionic liquid [Omim][PF6] and polyvinyl chloride. The inclusion of glutaraldehyde as a crosslinker improved the properties of the ionogel, allowing the enzymatic derivative to reach 5-fold higher activity than the free enzyme and also allowing it to be reused at 70 °C. The new methodology allows enzymatic derivatives to be designed by changing the ionic liquid, thus providing a suitable microenvironment for the enzyme. The ionic liquid may act on substrates to increase their local concentration, while reducing water activity in the enzyme’s microenvironment. All this allows the activity and selectivity of the enzyme to be improved and greener processes to be developed. The chemical composition and morphology of the ionogel were also studied by scanning electron microscopy–energy dispersive X-ray spectroscopy, finding that porosity, which was related with the chemical composition, was a key factor for the enzyme activity. |
format |
Text |
author |
Alfonso Escudero Antonia Pérez de los Ríos Carlos Godínez Francisca Tomás Francisco José Hernández-Fernández |
author_facet |
Alfonso Escudero Antonia Pérez de los Ríos Carlos Godínez Francisca Tomás Francisco José Hernández-Fernández |
author_sort |
Alfonso Escudero |
title |
Immobilization in Ionogel: A New Way to Improve the Activity and Stability of Candida antarctica Lipase B |
title_short |
Immobilization in Ionogel: A New Way to Improve the Activity and Stability of Candida antarctica Lipase B |
title_full |
Immobilization in Ionogel: A New Way to Improve the Activity and Stability of Candida antarctica Lipase B |
title_fullStr |
Immobilization in Ionogel: A New Way to Improve the Activity and Stability of Candida antarctica Lipase B |
title_full_unstemmed |
Immobilization in Ionogel: A New Way to Improve the Activity and Stability of Candida antarctica Lipase B |
title_sort |
immobilization in ionogel: a new way to improve the activity and stability of candida antarctica lipase b |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2020 |
url |
https://doi.org/10.3390/molecules25143233 |
op_coverage |
agris |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Molecules; Volume 25; Issue 14; Pages: 3233 |
op_relation |
Green Chemistry https://dx.doi.org/10.3390/molecules25143233 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/molecules25143233 |
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Molecules |
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25 |
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14 |
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3233 |
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1774713082169262080 |