Isolation and Characterization of a Novel Sialoglycopeptide Promoting Osteogenesis from Gadus morhua Eggs
Gadus morhua eggs contain several nutrients, including polyunsaturated fatty acids, lecithin and glycoproteins. A novel sialoglycopeptide from the eggs of G. morhua (Gm-SGPP) was extracted with 90% phenol and purified by Q Sepharose Fast Flow (QFF) ion exchange chromatography, followed by S-300 gel...
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ftmdpi:oai:mdpi.com:/1420-3049/25/1/156/ 2023-08-20T04:06:37+02:00 Isolation and Characterization of a Novel Sialoglycopeptide Promoting Osteogenesis from Gadus morhua Eggs Zhiliang Hei Meihui Zhao Yingying Tian Hong Chang Xuanri Shen Guanghua Xia Jingfeng Wang agris 2019-12-30 application/pdf https://doi.org/10.3390/molecules25010156 EN eng Multidisciplinary Digital Publishing Institute https://dx.doi.org/10.3390/molecules25010156 https://creativecommons.org/licenses/by/4.0/ Molecules; Volume 25; Issue 1; Pages: 156 Gadus morhua eggs sialoglycopeptide osteogenesis structure characterization Text 2019 ftmdpi https://doi.org/10.3390/molecules25010156 2023-07-31T22:57:02Z Gadus morhua eggs contain several nutrients, including polyunsaturated fatty acids, lecithin and glycoproteins. A novel sialoglycopeptide from the eggs of G. morhua (Gm-SGPP) was extracted with 90% phenol and purified by Q Sepharose Fast Flow (QFF) ion exchange chromatography, followed by S-300 gel filtration chromatography. Gm-SGPP contained 63.7% carbohydrate, 16.2% protein and 18.6% N-acetylneuraminic acid. High-performance size exclusion chromatography and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) demonstrated that Gm-SGPP is a 7000-Da pure sialoglycopeptide. β-elimination reaction suggested that Gm-SGPP contained N-glycan units. Amino acid N-terminal sequence analysis indicated the presence of Ala-Ser-Asn-Gly-Thr-Gln-Ala-Pro amino acid sequence. Moreover, N-glycan was connected at the third Asn location of the peptide chain through GlcNAc. Gm-SGPP was composed of D-mannose, D-glucuronic acid and D-galactose. Fourier transform-infrared spectroscopy (FT-IR), 1H-nuclear magnetic resonance spectroscopy (1H-NMR) and methylation analysis were performed to reveal the structure profile of Gm-SGPP. In vitro results showed that the proliferation activity of MC3T3-E1 cells was significantly promoted by Gm-SGPP. In vivo data revealed that Gm-SGPP increased the calcium and phosphorus content of tibias and promoted longitudinal bone growth in adolescent rats. Text Gadus morhua MDPI Open Access Publishing Molecules 25 1 156 |
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Gadus morhua eggs sialoglycopeptide osteogenesis structure characterization |
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Gadus morhua eggs sialoglycopeptide osteogenesis structure characterization Zhiliang Hei Meihui Zhao Yingying Tian Hong Chang Xuanri Shen Guanghua Xia Jingfeng Wang Isolation and Characterization of a Novel Sialoglycopeptide Promoting Osteogenesis from Gadus morhua Eggs |
topic_facet |
Gadus morhua eggs sialoglycopeptide osteogenesis structure characterization |
description |
Gadus morhua eggs contain several nutrients, including polyunsaturated fatty acids, lecithin and glycoproteins. A novel sialoglycopeptide from the eggs of G. morhua (Gm-SGPP) was extracted with 90% phenol and purified by Q Sepharose Fast Flow (QFF) ion exchange chromatography, followed by S-300 gel filtration chromatography. Gm-SGPP contained 63.7% carbohydrate, 16.2% protein and 18.6% N-acetylneuraminic acid. High-performance size exclusion chromatography and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) demonstrated that Gm-SGPP is a 7000-Da pure sialoglycopeptide. β-elimination reaction suggested that Gm-SGPP contained N-glycan units. Amino acid N-terminal sequence analysis indicated the presence of Ala-Ser-Asn-Gly-Thr-Gln-Ala-Pro amino acid sequence. Moreover, N-glycan was connected at the third Asn location of the peptide chain through GlcNAc. Gm-SGPP was composed of D-mannose, D-glucuronic acid and D-galactose. Fourier transform-infrared spectroscopy (FT-IR), 1H-nuclear magnetic resonance spectroscopy (1H-NMR) and methylation analysis were performed to reveal the structure profile of Gm-SGPP. In vitro results showed that the proliferation activity of MC3T3-E1 cells was significantly promoted by Gm-SGPP. In vivo data revealed that Gm-SGPP increased the calcium and phosphorus content of tibias and promoted longitudinal bone growth in adolescent rats. |
format |
Text |
author |
Zhiliang Hei Meihui Zhao Yingying Tian Hong Chang Xuanri Shen Guanghua Xia Jingfeng Wang |
author_facet |
Zhiliang Hei Meihui Zhao Yingying Tian Hong Chang Xuanri Shen Guanghua Xia Jingfeng Wang |
author_sort |
Zhiliang Hei |
title |
Isolation and Characterization of a Novel Sialoglycopeptide Promoting Osteogenesis from Gadus morhua Eggs |
title_short |
Isolation and Characterization of a Novel Sialoglycopeptide Promoting Osteogenesis from Gadus morhua Eggs |
title_full |
Isolation and Characterization of a Novel Sialoglycopeptide Promoting Osteogenesis from Gadus morhua Eggs |
title_fullStr |
Isolation and Characterization of a Novel Sialoglycopeptide Promoting Osteogenesis from Gadus morhua Eggs |
title_full_unstemmed |
Isolation and Characterization of a Novel Sialoglycopeptide Promoting Osteogenesis from Gadus morhua Eggs |
title_sort |
isolation and characterization of a novel sialoglycopeptide promoting osteogenesis from gadus morhua eggs |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2019 |
url |
https://doi.org/10.3390/molecules25010156 |
op_coverage |
agris |
genre |
Gadus morhua |
genre_facet |
Gadus morhua |
op_source |
Molecules; Volume 25; Issue 1; Pages: 156 |
op_relation |
https://dx.doi.org/10.3390/molecules25010156 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/molecules25010156 |
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Molecules |
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25 |
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1 |
container_start_page |
156 |
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1774717836244025344 |