Anti-Bacterial Effect and Cytotoxicity Assessment of Lipid 430 Isolated from Algibacter sp.
Two bacterial isolates from the Barents Sea, both belonging to the genus Algibacter, were found to yield extracts with anti-bacterial bioactivity. Mass spectrometry guided dereplication and purification of the active extracts lead to the isolation of the same active principle in both extracts. The s...
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ftmdpi:oai:mdpi.com:/1420-3049/24/21/3991/ 2023-08-20T04:05:31+02:00 Anti-Bacterial Effect and Cytotoxicity Assessment of Lipid 430 Isolated from Algibacter sp. Yannik K.-H. Schneider Kine Ø. Hansen Johan Isaksson Sara Ullsten Espen H. Hansen Jeanette Hammer Andersen agris 2019-11-05 application/pdf https://doi.org/10.3390/molecules24213991 EN eng Multidisciplinary Digital Publishing Institute https://dx.doi.org/10.3390/molecules24213991 https://creativecommons.org/licenses/by/4.0/ Molecules; Volume 24; Issue 21; Pages: 3991 flavolipin marine bacteria natural products lipopeptides algibacter Text 2019 ftmdpi https://doi.org/10.3390/molecules24213991 2023-07-31T22:45:48Z Two bacterial isolates from the Barents Sea, both belonging to the genus Algibacter, were found to yield extracts with anti-bacterial bioactivity. Mass spectrometry guided dereplication and purification of the active extracts lead to the isolation of the same active principle in both extracts. The structure of the bioactive compound was identified via mass spectrometry and nuclear resonance spectroscopy and it turned out to be the known lipopeptide Lipid 430. We discovered and determined its previously unknown anti-bacterial activity against Streptococcus agalactiae and revealed a cytotoxic effect against the A2058 human melanoma cell line at significantly lower concentrations compared to its anti-bacterial concentration. Flow cytometry and microscopy investigations of the cytotoxicity against the melanoma cell line indicated that Lipid 430 did not cause immediate cell lysis. The experiments with melanoma cells suggest that the compound functions trough more complex pathways than acting as a simple detergent. Text Barents Sea MDPI Open Access Publishing Barents Sea Molecules 24 21 3991 |
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flavolipin marine bacteria natural products lipopeptides algibacter |
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flavolipin marine bacteria natural products lipopeptides algibacter Yannik K.-H. Schneider Kine Ø. Hansen Johan Isaksson Sara Ullsten Espen H. Hansen Jeanette Hammer Andersen Anti-Bacterial Effect and Cytotoxicity Assessment of Lipid 430 Isolated from Algibacter sp. |
topic_facet |
flavolipin marine bacteria natural products lipopeptides algibacter |
description |
Two bacterial isolates from the Barents Sea, both belonging to the genus Algibacter, were found to yield extracts with anti-bacterial bioactivity. Mass spectrometry guided dereplication and purification of the active extracts lead to the isolation of the same active principle in both extracts. The structure of the bioactive compound was identified via mass spectrometry and nuclear resonance spectroscopy and it turned out to be the known lipopeptide Lipid 430. We discovered and determined its previously unknown anti-bacterial activity against Streptococcus agalactiae and revealed a cytotoxic effect against the A2058 human melanoma cell line at significantly lower concentrations compared to its anti-bacterial concentration. Flow cytometry and microscopy investigations of the cytotoxicity against the melanoma cell line indicated that Lipid 430 did not cause immediate cell lysis. The experiments with melanoma cells suggest that the compound functions trough more complex pathways than acting as a simple detergent. |
format |
Text |
author |
Yannik K.-H. Schneider Kine Ø. Hansen Johan Isaksson Sara Ullsten Espen H. Hansen Jeanette Hammer Andersen |
author_facet |
Yannik K.-H. Schneider Kine Ø. Hansen Johan Isaksson Sara Ullsten Espen H. Hansen Jeanette Hammer Andersen |
author_sort |
Yannik K.-H. Schneider |
title |
Anti-Bacterial Effect and Cytotoxicity Assessment of Lipid 430 Isolated from Algibacter sp. |
title_short |
Anti-Bacterial Effect and Cytotoxicity Assessment of Lipid 430 Isolated from Algibacter sp. |
title_full |
Anti-Bacterial Effect and Cytotoxicity Assessment of Lipid 430 Isolated from Algibacter sp. |
title_fullStr |
Anti-Bacterial Effect and Cytotoxicity Assessment of Lipid 430 Isolated from Algibacter sp. |
title_full_unstemmed |
Anti-Bacterial Effect and Cytotoxicity Assessment of Lipid 430 Isolated from Algibacter sp. |
title_sort |
anti-bacterial effect and cytotoxicity assessment of lipid 430 isolated from algibacter sp. |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2019 |
url |
https://doi.org/10.3390/molecules24213991 |
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agris |
geographic |
Barents Sea |
geographic_facet |
Barents Sea |
genre |
Barents Sea |
genre_facet |
Barents Sea |
op_source |
Molecules; Volume 24; Issue 21; Pages: 3991 |
op_relation |
https://dx.doi.org/10.3390/molecules24213991 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/molecules24213991 |
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Molecules |
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24 |
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21 |
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3991 |
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