Application of Enzymatic Extracts from a CALB Standard Strain as Biocatalyst within the Context of Conventional Biodiesel Production Optimization
The application of biocatalysts in the transesterification process of triglycerides (TG) allows integrating the glycerol in the form of monoglyceride (MG), sharply increasing the yield and the environmental sustainability of the conventional biodiesel production process. This is known as Ecodiesel....
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ftmdpi:oai:mdpi.com:/1420-3049/22/11/2025/ 2023-08-20T04:01:35+02:00 Application of Enzymatic Extracts from a CALB Standard Strain as Biocatalyst within the Context of Conventional Biodiesel Production Optimization Carlos Luna Diego Luna Felipa M. Bautista Rafael Estevez Juan Calero Alejandro Posadillo Antonio A. Romero Enrique D. Sancho agris 2017-11-21 application/pdf https://doi.org/10.3390/molecules22112025 EN eng Multidisciplinary Digital Publishing Institute Green Chemistry https://dx.doi.org/10.3390/molecules22112025 https://creativecommons.org/licenses/by/4.0/ Molecules; Volume 22; Issue 11; Pages: 2025 biodiesel ecodiesel lipases CALB enzymatic extracts transesterification glycerol Text 2017 ftmdpi https://doi.org/10.3390/molecules22112025 2023-07-31T21:17:26Z The application of biocatalysts in the transesterification process of triglycerides (TG) allows integrating the glycerol in the form of monoglyceride (MG), sharply increasing the yield and the environmental sustainability of the conventional biodiesel production process. This is known as Ecodiesel. To overcome the inconvenient of the high cost of the currently employed highly purified commercial enzymes, the use of scarcely purified extracts obtained from standard strains of the same species of commercial lipases currently applied in this process is being investigated. Thus, Candida antarctica type B (CALB) was chosen to determine the optimal conditions of culture of this yeast. The standard strain was obtained from the Spanish Type Microbial Cultures Collection (CECT) and has been used to carry out several studies to elucidate its optimum growth conditions. Through a process of lyophilization with prior dialysis of the liquid cultures, the enzymatic extracts were obtained. The different obtained cultures have been applied as biocatalysts in the 1,3-selective transesterification reaction of sunflower oil with ethanol to obtain Ecodiesel (FAEE + MG). Selectivity and reaction yields were obtained by gas chromatography. Acceptable yields are obtained during the reaction time as well as in successive reactions, demonstrating the feasibility of using these CALB enzymatic extracts as biocatalysts. Text Antarc* Antarctica MDPI Open Access Publishing Molecules 22 11 2025 |
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Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
biodiesel ecodiesel lipases CALB enzymatic extracts transesterification glycerol |
spellingShingle |
biodiesel ecodiesel lipases CALB enzymatic extracts transesterification glycerol Carlos Luna Diego Luna Felipa M. Bautista Rafael Estevez Juan Calero Alejandro Posadillo Antonio A. Romero Enrique D. Sancho Application of Enzymatic Extracts from a CALB Standard Strain as Biocatalyst within the Context of Conventional Biodiesel Production Optimization |
topic_facet |
biodiesel ecodiesel lipases CALB enzymatic extracts transesterification glycerol |
description |
The application of biocatalysts in the transesterification process of triglycerides (TG) allows integrating the glycerol in the form of monoglyceride (MG), sharply increasing the yield and the environmental sustainability of the conventional biodiesel production process. This is known as Ecodiesel. To overcome the inconvenient of the high cost of the currently employed highly purified commercial enzymes, the use of scarcely purified extracts obtained from standard strains of the same species of commercial lipases currently applied in this process is being investigated. Thus, Candida antarctica type B (CALB) was chosen to determine the optimal conditions of culture of this yeast. The standard strain was obtained from the Spanish Type Microbial Cultures Collection (CECT) and has been used to carry out several studies to elucidate its optimum growth conditions. Through a process of lyophilization with prior dialysis of the liquid cultures, the enzymatic extracts were obtained. The different obtained cultures have been applied as biocatalysts in the 1,3-selective transesterification reaction of sunflower oil with ethanol to obtain Ecodiesel (FAEE + MG). Selectivity and reaction yields were obtained by gas chromatography. Acceptable yields are obtained during the reaction time as well as in successive reactions, demonstrating the feasibility of using these CALB enzymatic extracts as biocatalysts. |
format |
Text |
author |
Carlos Luna Diego Luna Felipa M. Bautista Rafael Estevez Juan Calero Alejandro Posadillo Antonio A. Romero Enrique D. Sancho |
author_facet |
Carlos Luna Diego Luna Felipa M. Bautista Rafael Estevez Juan Calero Alejandro Posadillo Antonio A. Romero Enrique D. Sancho |
author_sort |
Carlos Luna |
title |
Application of Enzymatic Extracts from a CALB Standard Strain as Biocatalyst within the Context of Conventional Biodiesel Production Optimization |
title_short |
Application of Enzymatic Extracts from a CALB Standard Strain as Biocatalyst within the Context of Conventional Biodiesel Production Optimization |
title_full |
Application of Enzymatic Extracts from a CALB Standard Strain as Biocatalyst within the Context of Conventional Biodiesel Production Optimization |
title_fullStr |
Application of Enzymatic Extracts from a CALB Standard Strain as Biocatalyst within the Context of Conventional Biodiesel Production Optimization |
title_full_unstemmed |
Application of Enzymatic Extracts from a CALB Standard Strain as Biocatalyst within the Context of Conventional Biodiesel Production Optimization |
title_sort |
application of enzymatic extracts from a calb standard strain as biocatalyst within the context of conventional biodiesel production optimization |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2017 |
url |
https://doi.org/10.3390/molecules22112025 |
op_coverage |
agris |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Molecules; Volume 22; Issue 11; Pages: 2025 |
op_relation |
Green Chemistry https://dx.doi.org/10.3390/molecules22112025 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/molecules22112025 |
container_title |
Molecules |
container_volume |
22 |
container_issue |
11 |
container_start_page |
2025 |
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1774724830230216704 |