Regioselective Palmitoylation of 9-(2,3-Dihydroxy- propyl)adenine Catalyzed by a Glycopolymer-enzyme Conjugate

• Published in: Molecules
• Main Authors: Jana Brabcová, Jiří Blažek, Marcela Krečmerová, Jiří Vondrášek, Jose Palomo, Marie Zarevúcka
• Format: Text
• Language: English
• Published: Multidisciplinary Digital Publishing Institute 2016
• Subjects: regioselectivity; palmitoylation; glycosylation; chemical modification; Antarc*; Antarctica
• Online Access: https://doi.org/10.3390/molecules21050648

The enzymatic regioselective monopalmitoylation of racemic 9-(2,3-dihydroxypropyl)- adenine (DHPA), an approved antiviral agent, has been performed by an immobilized form of Candida antarctica B lipase (CAL-B) using a 4:1 DMF/hexane mixture as the reaction medium. To improve the chemical yield of the desired monopalmitoylation reaction, solid-phase chemical modifications of the lipase were evaluated. The reaction yield was successfully increased obtaining 100% product after a second treatment of the product solution with fresh immobilised chemically glycosylated-CAL-B.