Reversible Immobilization of Lipases on Heterofunctional Octyl-Amino Agarose Beads Prevents Enzyme Desorption

• Published in: Molecules
• Main Authors: Nazzoly Rueda, Tiago Albuquerque, Rocio Bartolome-Cabrero, Laura Fernandez-Lopez, Rodrigo Torres, Claudia Ortiz, Jose Dos Santos, Oveimar Barbosa, Roberto Fernandez-Lafuente
• Format: Text
• Language: English
• Published: Multidisciplinary Digital Publishing Institute 2016
• Subjects: heterofunctional supports; octyl supports; interfacial activation of lipases; ion exchange; enzyme hyperactivation; reversible immobilization; Rugosa; Triton; Antarc*; Antarctica
• Online Access: https://doi.org/10.3390/molecules21050646

Two different heterofunctional octyl-amino supports have been prepared using ethylenediamine and hexylendiamine (OCEDA and OCHDA) and utilized to immobilize five lipases (lipases A (CALA) and B (CALB) from Candida antarctica, lipases from Thermomyces lanuginosus (TLL), from Rhizomucor miehei (RML) a...