Influence of the Morphology of Core-Shell Supports on the Immobilization of Lipase B from Candida antarctica
Core-shell polymer particles with different properties were produced through combined suspension-emulsion polymerizations and employed as supports for immobilization of lipase B from Candida antarctica. In order to evaluate how the morphology of the particles affects the immobilization parameters, e...
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ftmdpi:oai:mdpi.com:/1420-3049/19/8/12509/ 2023-08-20T04:01:58+02:00 Influence of the Morphology of Core-Shell Supports on the Immobilization of Lipase B from Candida antarctica Martina Pinto Denise Freire José Pinto agris 2014-08-18 application/pdf https://doi.org/10.3390/molecules190812509 EN eng Multidisciplinary Digital Publishing Institute Molecular Diversity https://dx.doi.org/10.3390/molecules190812509 https://creativecommons.org/licenses/by/3.0/ Molecules; Volume 19; Issue 8; Pages: 12509-12530 core-shell particles polystyrene immobilization lipase B from Candida antarctica Text 2014 ftmdpi https://doi.org/10.3390/molecules190812509 2023-07-31T20:38:50Z Core-shell polymer particles with different properties were produced through combined suspension-emulsion polymerizations and employed as supports for immobilization of lipase B from Candida antarctica. In order to evaluate how the morphology of the particles affects the immobilization parameters, empirical models were developed to describe the performance of the biocatalysts as a function of the specific area, volume of pores and average pore diameter of the supports. It was observed that the average pore sizes did not affect the enzymatic activities in the analyzed range of pore sizes. It was also observed that the increase of the specific area (and of the volume of pores) led to higher enzyme loadings, also leading to an increase in the esterification activity, as expected. However, when the specific area (and volume of pores) increased, the hydrolytic activity and the retention of hydrolytic activity of the biocatalysts decreased, indicating the existence of diffusional limitations for some hydrolytic reactions, probably because of the high reaction rates. Text Antarc* Antarctica MDPI Open Access Publishing Molecules 19 8 12509 12530 |
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Open Polar |
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MDPI Open Access Publishing |
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ftmdpi |
language |
English |
topic |
core-shell particles polystyrene immobilization lipase B from Candida antarctica |
spellingShingle |
core-shell particles polystyrene immobilization lipase B from Candida antarctica Martina Pinto Denise Freire José Pinto Influence of the Morphology of Core-Shell Supports on the Immobilization of Lipase B from Candida antarctica |
topic_facet |
core-shell particles polystyrene immobilization lipase B from Candida antarctica |
description |
Core-shell polymer particles with different properties were produced through combined suspension-emulsion polymerizations and employed as supports for immobilization of lipase B from Candida antarctica. In order to evaluate how the morphology of the particles affects the immobilization parameters, empirical models were developed to describe the performance of the biocatalysts as a function of the specific area, volume of pores and average pore diameter of the supports. It was observed that the average pore sizes did not affect the enzymatic activities in the analyzed range of pore sizes. It was also observed that the increase of the specific area (and of the volume of pores) led to higher enzyme loadings, also leading to an increase in the esterification activity, as expected. However, when the specific area (and volume of pores) increased, the hydrolytic activity and the retention of hydrolytic activity of the biocatalysts decreased, indicating the existence of diffusional limitations for some hydrolytic reactions, probably because of the high reaction rates. |
format |
Text |
author |
Martina Pinto Denise Freire José Pinto |
author_facet |
Martina Pinto Denise Freire José Pinto |
author_sort |
Martina Pinto |
title |
Influence of the Morphology of Core-Shell Supports on the Immobilization of Lipase B from Candida antarctica |
title_short |
Influence of the Morphology of Core-Shell Supports on the Immobilization of Lipase B from Candida antarctica |
title_full |
Influence of the Morphology of Core-Shell Supports on the Immobilization of Lipase B from Candida antarctica |
title_fullStr |
Influence of the Morphology of Core-Shell Supports on the Immobilization of Lipase B from Candida antarctica |
title_full_unstemmed |
Influence of the Morphology of Core-Shell Supports on the Immobilization of Lipase B from Candida antarctica |
title_sort |
influence of the morphology of core-shell supports on the immobilization of lipase b from candida antarctica |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2014 |
url |
https://doi.org/10.3390/molecules190812509 |
op_coverage |
agris |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Molecules; Volume 19; Issue 8; Pages: 12509-12530 |
op_relation |
Molecular Diversity https://dx.doi.org/10.3390/molecules190812509 |
op_rights |
https://creativecommons.org/licenses/by/3.0/ |
op_doi |
https://doi.org/10.3390/molecules190812509 |
container_title |
Molecules |
container_volume |
19 |
container_issue |
8 |
container_start_page |
12509 |
op_container_end_page |
12530 |
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1774712355691692032 |