Combined Effects of Ultrasound and Immobilization Protocol on Butyl Acetate Synthesis Catalyzed by CALB
It is well established that the performance of lipase B from Candida antarctica (CALB) as catalyst for esterification reactions may be improved by the use of ultrasound technology or by its immobilization on styrene-divinylbenzene beads (MCI-CALB). The present research evaluated the synthesis of but...
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ftmdpi:oai:mdpi.com:/1420-3049/19/7/9562/ 2023-08-20T04:02:34+02:00 Combined Effects of Ultrasound and Immobilization Protocol on Butyl Acetate Synthesis Catalyzed by CALB Joana Alves Cristina Garcia-Galan Mirela Schein Alexandre Silva Oveimar Barbosa Marco Ayub Roberto Fernandez-Lafuente Rafael Rodrigues agris 2014-07-07 application/pdf https://doi.org/10.3390/molecules19079562 EN eng Multidisciplinary Digital Publishing Institute Molecular Diversity https://dx.doi.org/10.3390/molecules19079562 https://creativecommons.org/licenses/by/3.0/ Molecules; Volume 19; Issue 7; Pages: 9562-9576 esterification lipase ultrasound enzyme reuse enzyme stability butyl acetate Text 2014 ftmdpi https://doi.org/10.3390/molecules19079562 2023-07-31T20:38:10Z It is well established that the performance of lipase B from Candida antarctica (CALB) as catalyst for esterification reactions may be improved by the use of ultrasound technology or by its immobilization on styrene-divinylbenzene beads (MCI-CALB). The present research evaluated the synthesis of butyl acetate using MCI-CALB under ultrasonic energy, comparing the results against those obtained using the commercial preparation, Novozym 435. The optimal conditions were determined using response surface methodology (RSM) evaluating the following parameters: reaction temperature, substrate molar ratio, amount of biocatalyst, and added water. The optimal conditions for butyl acetate synthesis catalyzed by MCI-CALB were: temperature, 48.8 °C; substrate molar ratio, 3.46:1 alcohol:acid; amount of biocatalyst, 7.5%; and added water 0.28%, both as substrate mass. Under these conditions, 90% of conversion was reached in 1.5 h. In terms of operational stability, MCI-CALB was reused in seven cycles while keeping 70% of its initial activity under ultrasonic energy. The support pore size and resistance are key points for the enzyme activity and stability under mechanical stirring. The use of ultrasound improved both activity and stability because of better homogeneity and reduced mechanical stress to the immobilized system. Text Antarc* Antarctica MDPI Open Access Publishing Molecules 19 7 9562 9576 |
institution |
Open Polar |
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MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
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English |
topic |
esterification lipase ultrasound enzyme reuse enzyme stability butyl acetate |
spellingShingle |
esterification lipase ultrasound enzyme reuse enzyme stability butyl acetate Joana Alves Cristina Garcia-Galan Mirela Schein Alexandre Silva Oveimar Barbosa Marco Ayub Roberto Fernandez-Lafuente Rafael Rodrigues Combined Effects of Ultrasound and Immobilization Protocol on Butyl Acetate Synthesis Catalyzed by CALB |
topic_facet |
esterification lipase ultrasound enzyme reuse enzyme stability butyl acetate |
description |
It is well established that the performance of lipase B from Candida antarctica (CALB) as catalyst for esterification reactions may be improved by the use of ultrasound technology or by its immobilization on styrene-divinylbenzene beads (MCI-CALB). The present research evaluated the synthesis of butyl acetate using MCI-CALB under ultrasonic energy, comparing the results against those obtained using the commercial preparation, Novozym 435. The optimal conditions were determined using response surface methodology (RSM) evaluating the following parameters: reaction temperature, substrate molar ratio, amount of biocatalyst, and added water. The optimal conditions for butyl acetate synthesis catalyzed by MCI-CALB were: temperature, 48.8 °C; substrate molar ratio, 3.46:1 alcohol:acid; amount of biocatalyst, 7.5%; and added water 0.28%, both as substrate mass. Under these conditions, 90% of conversion was reached in 1.5 h. In terms of operational stability, MCI-CALB was reused in seven cycles while keeping 70% of its initial activity under ultrasonic energy. The support pore size and resistance are key points for the enzyme activity and stability under mechanical stirring. The use of ultrasound improved both activity and stability because of better homogeneity and reduced mechanical stress to the immobilized system. |
format |
Text |
author |
Joana Alves Cristina Garcia-Galan Mirela Schein Alexandre Silva Oveimar Barbosa Marco Ayub Roberto Fernandez-Lafuente Rafael Rodrigues |
author_facet |
Joana Alves Cristina Garcia-Galan Mirela Schein Alexandre Silva Oveimar Barbosa Marco Ayub Roberto Fernandez-Lafuente Rafael Rodrigues |
author_sort |
Joana Alves |
title |
Combined Effects of Ultrasound and Immobilization Protocol on Butyl Acetate Synthesis Catalyzed by CALB |
title_short |
Combined Effects of Ultrasound and Immobilization Protocol on Butyl Acetate Synthesis Catalyzed by CALB |
title_full |
Combined Effects of Ultrasound and Immobilization Protocol on Butyl Acetate Synthesis Catalyzed by CALB |
title_fullStr |
Combined Effects of Ultrasound and Immobilization Protocol on Butyl Acetate Synthesis Catalyzed by CALB |
title_full_unstemmed |
Combined Effects of Ultrasound and Immobilization Protocol on Butyl Acetate Synthesis Catalyzed by CALB |
title_sort |
combined effects of ultrasound and immobilization protocol on butyl acetate synthesis catalyzed by calb |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2014 |
url |
https://doi.org/10.3390/molecules19079562 |
op_coverage |
agris |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Molecules; Volume 19; Issue 7; Pages: 9562-9576 |
op_relation |
Molecular Diversity https://dx.doi.org/10.3390/molecules19079562 |
op_rights |
https://creativecommons.org/licenses/by/3.0/ |
op_doi |
https://doi.org/10.3390/molecules19079562 |
container_title |
Molecules |
container_volume |
19 |
container_issue |
7 |
container_start_page |
9562 |
op_container_end_page |
9576 |
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1774713087548456960 |