Klebsiella pneumoniae in New Zealand sea lions : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Veterinary Science at Massey University, Manawatū, New Zealand

Klebsiella pneumoniae has been circulating in New Zealand sea lions since the outbreaks during the breeding seasons of 2001/02 and 2002/03 in Sandy Bay, on Enderby Island, Auckland Islands. A large number of pups have since died from K. pneumoniae every year during the breeding season. In order to p...

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Main Author: Pinpimai, Komkiew
Format: Thesis
Language:English
Published: Massey University 2018
Subjects:
Online Access:http://hdl.handle.net/10179/15260
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spelling ftmasseyuniv:oai:mro.massey.ac.nz:10179/15260 2023-05-15T15:33:43+02:00 Klebsiella pneumoniae in New Zealand sea lions : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Veterinary Science at Massey University, Manawatū, New Zealand Pinpimai, Komkiew 2018 http://hdl.handle.net/10179/15260 en eng Massey University http://hdl.handle.net/10179/15260 Q112189692 The Author Hooker's sea lion Pathogens New Zealand Infections Prevention Klebsiella pneumoniae Thesis 2018 ftmasseyuniv 2022-08-09T17:16:00Z Klebsiella pneumoniae has been circulating in New Zealand sea lions since the outbreaks during the breeding seasons of 2001/02 and 2002/03 in Sandy Bay, on Enderby Island, Auckland Islands. A large number of pups have since died from K. pneumoniae every year during the breeding season. In order to prevent and control this infection, baseline data including bacterial phenotype and genotype, geographic distribution of the pathogen, and the immune response to the pathogen, have to be established. In this study, hypervirulent (HV) K. pneumoniae was isolated from different sources including New Zealand sea lion (NZSL) pups from different breeding sites, and characterised using a combination of biochemical, phenotypic tests, serological analysis and genotyping via whole genome sequencing. Isolates from pups, substrate samples from different breeding sites, a NZSL adult and birds, all had a close genetic relationship. The isolates have the same basic characteristics including a hypermucoviscous phenotype, serotype 2, and sequence type 86. This suggested clonality of this pathogen. The geographic distribution of the pathogen was found to be Enderby Island, Dundas Island, Campbell Island, and the Otago Peninsula (New Zealand mainland). The isolates analysed were all susceptible to commonly used antibiotics, with the exception of ampicillin. The HV isolates from pups were able to utilise a wide panel of carbon and nitrogen sources and had activity in a wide range of pH from 4.5 to 10, supporting the ability of this pathogen to survive in diverse environments. The findings in this thesis also suggest that the environment can be a reservoir for a short time period. For the long term, between breeding seasons, New Zealand sea lion adults and birds that live around the breeding site are potential reservoirs. The HV isolates from pups were resistant to some innate immune responses, including serum killing ability, oxidative killing ability and phagocytosis by neutrophils and monocytes. Overall, this study provided phenotypic ... Thesis Auckland Islands Enderby Island Dundas Island Massey University: Massey Research Online New Zealand Campbell Island ENVELOPE(169.500,169.500,-52.500,-52.500)
institution Open Polar
collection Massey University: Massey Research Online
op_collection_id ftmasseyuniv
language English
topic Hooker's sea lion
Pathogens
New Zealand
Infections
Prevention
Klebsiella pneumoniae
spellingShingle Hooker's sea lion
Pathogens
New Zealand
Infections
Prevention
Klebsiella pneumoniae
Pinpimai, Komkiew
Klebsiella pneumoniae in New Zealand sea lions : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Veterinary Science at Massey University, Manawatū, New Zealand
topic_facet Hooker's sea lion
Pathogens
New Zealand
Infections
Prevention
Klebsiella pneumoniae
description Klebsiella pneumoniae has been circulating in New Zealand sea lions since the outbreaks during the breeding seasons of 2001/02 and 2002/03 in Sandy Bay, on Enderby Island, Auckland Islands. A large number of pups have since died from K. pneumoniae every year during the breeding season. In order to prevent and control this infection, baseline data including bacterial phenotype and genotype, geographic distribution of the pathogen, and the immune response to the pathogen, have to be established. In this study, hypervirulent (HV) K. pneumoniae was isolated from different sources including New Zealand sea lion (NZSL) pups from different breeding sites, and characterised using a combination of biochemical, phenotypic tests, serological analysis and genotyping via whole genome sequencing. Isolates from pups, substrate samples from different breeding sites, a NZSL adult and birds, all had a close genetic relationship. The isolates have the same basic characteristics including a hypermucoviscous phenotype, serotype 2, and sequence type 86. This suggested clonality of this pathogen. The geographic distribution of the pathogen was found to be Enderby Island, Dundas Island, Campbell Island, and the Otago Peninsula (New Zealand mainland). The isolates analysed were all susceptible to commonly used antibiotics, with the exception of ampicillin. The HV isolates from pups were able to utilise a wide panel of carbon and nitrogen sources and had activity in a wide range of pH from 4.5 to 10, supporting the ability of this pathogen to survive in diverse environments. The findings in this thesis also suggest that the environment can be a reservoir for a short time period. For the long term, between breeding seasons, New Zealand sea lion adults and birds that live around the breeding site are potential reservoirs. The HV isolates from pups were resistant to some innate immune responses, including serum killing ability, oxidative killing ability and phagocytosis by neutrophils and monocytes. Overall, this study provided phenotypic ...
format Thesis
author Pinpimai, Komkiew
author_facet Pinpimai, Komkiew
author_sort Pinpimai, Komkiew
title Klebsiella pneumoniae in New Zealand sea lions : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Veterinary Science at Massey University, Manawatū, New Zealand
title_short Klebsiella pneumoniae in New Zealand sea lions : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Veterinary Science at Massey University, Manawatū, New Zealand
title_full Klebsiella pneumoniae in New Zealand sea lions : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Veterinary Science at Massey University, Manawatū, New Zealand
title_fullStr Klebsiella pneumoniae in New Zealand sea lions : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Veterinary Science at Massey University, Manawatū, New Zealand
title_full_unstemmed Klebsiella pneumoniae in New Zealand sea lions : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Veterinary Science at Massey University, Manawatū, New Zealand
title_sort klebsiella pneumoniae in new zealand sea lions : a thesis presented in partial fulfilment of the requirements for the degree of doctor of philosophy in veterinary science at massey university, manawatū, new zealand
publisher Massey University
publishDate 2018
url http://hdl.handle.net/10179/15260
long_lat ENVELOPE(169.500,169.500,-52.500,-52.500)
geographic New Zealand
Campbell Island
geographic_facet New Zealand
Campbell Island
genre Auckland Islands
Enderby Island
Dundas Island
genre_facet Auckland Islands
Enderby Island
Dundas Island
op_relation http://hdl.handle.net/10179/15260
Q112189692
op_rights The Author
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