First record of the endophytic bacteria of Deschampsia antarctica Ė. Desv. from two distant localities of the maritime Antarctic
Endophytic bacteria, recognized for their beneficial effects on plant development and adaptation, can facilitate the survival of Antarctic plants in severe environments. Here we studied endophytes of the vascular plant Deschampsia antarctica Ė. Desv. from two distantly located regions in the maritim...
Main Authors: | , , , , , , , , , , , , , |
---|---|
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Masaryk Univerzity
2021
|
Subjects: | |
Online Access: | http://journals.muni.cz/CPR/article/view/15422 |
id |
ftmasarykunivojs:oai:ojs.journals.muni.cz:article/15422 |
---|---|
record_format |
openpolar |
spelling |
ftmasarykunivojs:oai:ojs.journals.muni.cz:article/15422 2023-05-15T13:46:41+02:00 First record of the endophytic bacteria of Deschampsia antarctica Ė. Desv. from two distant localities of the maritime Antarctic Podolich, Olga Prekrasna, Ievgeniia Parnikoza, Ivan Voznyuk, Tamara Zubova, Ganna Zaets, Iryna Miryuta, Natalia Myryuta, Ganna Poronnik, Oksana Kozeretska, Iryna Kunakh, Viktor Pirttila, Anna Maria Dykyi, Evgen Kozyrovska, Natalia 2021-08-24 application/pdf http://journals.muni.cz/CPR/article/view/15422 eng eng Masaryk Univerzity http://journals.muni.cz/CPR/article/view/15422/12290 http://journals.muni.cz/CPR/article/view/15422 Copyright (c) 2020 Czech Polar Reports https://creativecommons.org/licenses/by-nc-nd/4.0 CC-BY-NC-ND Czech Polar Reports; Vol 11 No 1 (2021); 134-153 Czech Polar Reports; Vol. 11 No. 1 (2021); 134-153 1805-0697 1805-0689 endophytic bacteria Antarctic hairgrass Antarctica plant growth promotion info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2021 ftmasarykunivojs 2022-06-26T10:16:45Z Endophytic bacteria, recognized for their beneficial effects on plant development and adaptation, can facilitate the survival of Antarctic plants in severe environments. Here we studied endophytes of the vascular plant Deschampsia antarctica Ė. Desv. from two distantly located regions in the maritime Antarctic: King George Island (South Shetland Islands) and Galindez Island (Argentine Islands). Bacterial group-specific PCR indicated presence of Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, Firmicutes, Cytophaga-Flavobacteria and Actinobacteria in root and leaf endosphere of D. antarctica sampled at four distinct sites of both locations. The diversity of endophytic bacteria was significantly higher in the leaves compared to the roots in plants from Galindez Island. Similarly, the diversity of endophytes was higher in the leaves rather than roots of plants from the King George Island. Twelve bacterial species were isolated from roots of D. antarctica of Galindez Island (the Karpaty Ridge and the Meteo Point) and identified by sequencing the 16S rRNA gene. Isolates were dominated by the Pseudomonas genus, followed by the genera Bacillus and Micrococcus. The vast majority of the isolates exhibited cellulase and pectinase activities, however, Bacillus spp. expressed neither of them, suggesting lack of genetic flow of these traits in endophytic bacilli in the maritime Antarctic. Pseudomonas sp. IMBG305 promoted an increase in the leaf number in most of the treated plant genotypes when compared with non-inoculated plants, and a rapid vegetation period of D. antarctica cultured in vitro, albeit the length of leaves in the treated plants was significantly lower, and flavonoid content leveled off in all treated plants. D. antarctica is known to develop diverse ecotypes with regard to ecological conditions, such as organic input, moisture or wind exposition. The D. antarctica phenotype could be extended further through the endophyte colonization, since phenotypic changes were observed in the inoculated D. ... Article in Journal/Newspaper Antarc* Antarctic Antarctica Argentine Islands Galindez Island King George Island South Shetland Islands Masaryk University Journals Antarctic King George Island South Shetland Islands Argentine Argentine Islands ENVELOPE(-64.273,-64.273,-65.246,-65.246) Galindez ENVELOPE(-64.248,-64.248,-65.250,-65.250) Galindez Island ENVELOPE(-64.267,-64.267,-65.250,-65.250) |
institution |
Open Polar |
collection |
Masaryk University Journals |
op_collection_id |
ftmasarykunivojs |
language |
English |
topic |
endophytic bacteria Antarctic hairgrass Antarctica plant growth promotion |
spellingShingle |
endophytic bacteria Antarctic hairgrass Antarctica plant growth promotion Podolich, Olga Prekrasna, Ievgeniia Parnikoza, Ivan Voznyuk, Tamara Zubova, Ganna Zaets, Iryna Miryuta, Natalia Myryuta, Ganna Poronnik, Oksana Kozeretska, Iryna Kunakh, Viktor Pirttila, Anna Maria Dykyi, Evgen Kozyrovska, Natalia First record of the endophytic bacteria of Deschampsia antarctica Ė. Desv. from two distant localities of the maritime Antarctic |
topic_facet |
endophytic bacteria Antarctic hairgrass Antarctica plant growth promotion |
description |
Endophytic bacteria, recognized for their beneficial effects on plant development and adaptation, can facilitate the survival of Antarctic plants in severe environments. Here we studied endophytes of the vascular plant Deschampsia antarctica Ė. Desv. from two distantly located regions in the maritime Antarctic: King George Island (South Shetland Islands) and Galindez Island (Argentine Islands). Bacterial group-specific PCR indicated presence of Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, Firmicutes, Cytophaga-Flavobacteria and Actinobacteria in root and leaf endosphere of D. antarctica sampled at four distinct sites of both locations. The diversity of endophytic bacteria was significantly higher in the leaves compared to the roots in plants from Galindez Island. Similarly, the diversity of endophytes was higher in the leaves rather than roots of plants from the King George Island. Twelve bacterial species were isolated from roots of D. antarctica of Galindez Island (the Karpaty Ridge and the Meteo Point) and identified by sequencing the 16S rRNA gene. Isolates were dominated by the Pseudomonas genus, followed by the genera Bacillus and Micrococcus. The vast majority of the isolates exhibited cellulase and pectinase activities, however, Bacillus spp. expressed neither of them, suggesting lack of genetic flow of these traits in endophytic bacilli in the maritime Antarctic. Pseudomonas sp. IMBG305 promoted an increase in the leaf number in most of the treated plant genotypes when compared with non-inoculated plants, and a rapid vegetation period of D. antarctica cultured in vitro, albeit the length of leaves in the treated plants was significantly lower, and flavonoid content leveled off in all treated plants. D. antarctica is known to develop diverse ecotypes with regard to ecological conditions, such as organic input, moisture or wind exposition. The D. antarctica phenotype could be extended further through the endophyte colonization, since phenotypic changes were observed in the inoculated D. ... |
format |
Article in Journal/Newspaper |
author |
Podolich, Olga Prekrasna, Ievgeniia Parnikoza, Ivan Voznyuk, Tamara Zubova, Ganna Zaets, Iryna Miryuta, Natalia Myryuta, Ganna Poronnik, Oksana Kozeretska, Iryna Kunakh, Viktor Pirttila, Anna Maria Dykyi, Evgen Kozyrovska, Natalia |
author_facet |
Podolich, Olga Prekrasna, Ievgeniia Parnikoza, Ivan Voznyuk, Tamara Zubova, Ganna Zaets, Iryna Miryuta, Natalia Myryuta, Ganna Poronnik, Oksana Kozeretska, Iryna Kunakh, Viktor Pirttila, Anna Maria Dykyi, Evgen Kozyrovska, Natalia |
author_sort |
Podolich, Olga |
title |
First record of the endophytic bacteria of Deschampsia antarctica Ė. Desv. from two distant localities of the maritime Antarctic |
title_short |
First record of the endophytic bacteria of Deschampsia antarctica Ė. Desv. from two distant localities of the maritime Antarctic |
title_full |
First record of the endophytic bacteria of Deschampsia antarctica Ė. Desv. from two distant localities of the maritime Antarctic |
title_fullStr |
First record of the endophytic bacteria of Deschampsia antarctica Ė. Desv. from two distant localities of the maritime Antarctic |
title_full_unstemmed |
First record of the endophytic bacteria of Deschampsia antarctica Ė. Desv. from two distant localities of the maritime Antarctic |
title_sort |
first record of the endophytic bacteria of deschampsia antarctica ė. desv. from two distant localities of the maritime antarctic |
publisher |
Masaryk Univerzity |
publishDate |
2021 |
url |
http://journals.muni.cz/CPR/article/view/15422 |
long_lat |
ENVELOPE(-64.273,-64.273,-65.246,-65.246) ENVELOPE(-64.248,-64.248,-65.250,-65.250) ENVELOPE(-64.267,-64.267,-65.250,-65.250) |
geographic |
Antarctic King George Island South Shetland Islands Argentine Argentine Islands Galindez Galindez Island |
geographic_facet |
Antarctic King George Island South Shetland Islands Argentine Argentine Islands Galindez Galindez Island |
genre |
Antarc* Antarctic Antarctica Argentine Islands Galindez Island King George Island South Shetland Islands |
genre_facet |
Antarc* Antarctic Antarctica Argentine Islands Galindez Island King George Island South Shetland Islands |
op_source |
Czech Polar Reports; Vol 11 No 1 (2021); 134-153 Czech Polar Reports; Vol. 11 No. 1 (2021); 134-153 1805-0697 1805-0689 |
op_relation |
http://journals.muni.cz/CPR/article/view/15422/12290 http://journals.muni.cz/CPR/article/view/15422 |
op_rights |
Copyright (c) 2020 Czech Polar Reports https://creativecommons.org/licenses/by-nc-nd/4.0 |
op_rightsnorm |
CC-BY-NC-ND |
_version_ |
1766245089615544320 |