The effect of shock freezing on physiological properties and consequent growth of Antarctic filamentous (Stigeoclonium sp.) and coccal alga (Diplosphaera chodatii) on agar plates
In this study, we investigated the effects of shock freezing on physiological properties and consequent growth of in the Antarctic alga Stigeoclonium sp. and comparative coccal alga Diplosphaera chodatii on agar plates. Culture of algae grown in liquid medium were used to study subzero temperatures...
Main Authors: | , , , |
---|---|
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Masaryk Univerzity
2019
|
Subjects: | |
Online Access: | http://journals.muni.cz/CPR/article/view/12939 |
id |
ftmasarykunivojs:oai:ojs.journals.muni.cz:article/12939 |
---|---|
record_format |
openpolar |
spelling |
ftmasarykunivojs:oai:ojs.journals.muni.cz:article/12939 2023-05-15T13:46:41+02:00 The effect of shock freezing on physiological properties and consequent growth of Antarctic filamentous (Stigeoclonium sp.) and coccal alga (Diplosphaera chodatii) on agar plates Orekhova, Alla Barták, Miloš Özkar, Aleyna Elster, Josef 2019-01-01 application/pdf http://journals.muni.cz/CPR/article/view/12939 eng eng Masaryk Univerzity http://journals.muni.cz/CPR/article/view/12939/11291 http://journals.muni.cz/CPR/article/view/12939 Copyright (c) 2020 Czech Polar Reports https://creativecommons.org/licenses/by-nc-nd/4.0 CC-BY-NC-ND Czech Polar Reports; Vol 9 No 1 (2019); 37-48 Czech Polar Reports; Vol. 9 No. 1 (2019); 37-48 1805-0697 1805-0689 Antarctica cold stress cryoresistance algal cultures biotechnologies info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2019 ftmasarykunivojs 2022-06-26T10:16:39Z In this study, we investigated the effects of shock freezing on physiological properties and consequent growth of in the Antarctic alga Stigeoclonium sp. and comparative coccal alga Diplosphaera chodatii on agar plates. Culture of algae grown in liquid medium were used to study subzero temperatures on the species resistance to shock freezing. Then, microalgae were frozen in liquid nitrogen and inoculated on BBM agar after thawing. Physiological status of algae was evaluated by chlorophyll fluorescence parameters during 28 days. The results showed that interspecific differences existed in their tolerance to shock freezing, as well as their consequent growth rate on agars. Direct effects of freezing in liquid nitrogen was demonstrated in chlorophyll fluorescence parameters recorded immediately after thawing the samples (in liquid medium). In spite of the fact that majority of cells was destroyed by shock freezing, the potential of photochemical processes in PS II (FV/FM) remained constant in D. chodatii. It may indicate high resistance of the species to freezing/thawing cycles and a capability of the surviving cells, core chlorophylls in PS II respectively, to perform photosynthetic processes related to PS II. Contrastingly, Stigeoclonium sp. showed a shock freezing-dependent decrease in FV/FM. When shock-frozen, thawed and inoculated on agar plates, the culture of D. chodatii, and Stigeoclonium sp. showed cultivation time-dependent increase in chlorophyll fluorescence parameters (FV/FM, FS). Article in Journal/Newspaper Antarc* Antarctic Antarctica Masaryk University Journals Antarctic The Antarctic |
institution |
Open Polar |
collection |
Masaryk University Journals |
op_collection_id |
ftmasarykunivojs |
language |
English |
topic |
Antarctica cold stress cryoresistance algal cultures biotechnologies |
spellingShingle |
Antarctica cold stress cryoresistance algal cultures biotechnologies Orekhova, Alla Barták, Miloš Özkar, Aleyna Elster, Josef The effect of shock freezing on physiological properties and consequent growth of Antarctic filamentous (Stigeoclonium sp.) and coccal alga (Diplosphaera chodatii) on agar plates |
topic_facet |
Antarctica cold stress cryoresistance algal cultures biotechnologies |
description |
In this study, we investigated the effects of shock freezing on physiological properties and consequent growth of in the Antarctic alga Stigeoclonium sp. and comparative coccal alga Diplosphaera chodatii on agar plates. Culture of algae grown in liquid medium were used to study subzero temperatures on the species resistance to shock freezing. Then, microalgae were frozen in liquid nitrogen and inoculated on BBM agar after thawing. Physiological status of algae was evaluated by chlorophyll fluorescence parameters during 28 days. The results showed that interspecific differences existed in their tolerance to shock freezing, as well as their consequent growth rate on agars. Direct effects of freezing in liquid nitrogen was demonstrated in chlorophyll fluorescence parameters recorded immediately after thawing the samples (in liquid medium). In spite of the fact that majority of cells was destroyed by shock freezing, the potential of photochemical processes in PS II (FV/FM) remained constant in D. chodatii. It may indicate high resistance of the species to freezing/thawing cycles and a capability of the surviving cells, core chlorophylls in PS II respectively, to perform photosynthetic processes related to PS II. Contrastingly, Stigeoclonium sp. showed a shock freezing-dependent decrease in FV/FM. When shock-frozen, thawed and inoculated on agar plates, the culture of D. chodatii, and Stigeoclonium sp. showed cultivation time-dependent increase in chlorophyll fluorescence parameters (FV/FM, FS). |
format |
Article in Journal/Newspaper |
author |
Orekhova, Alla Barták, Miloš Özkar, Aleyna Elster, Josef |
author_facet |
Orekhova, Alla Barták, Miloš Özkar, Aleyna Elster, Josef |
author_sort |
Orekhova, Alla |
title |
The effect of shock freezing on physiological properties and consequent growth of Antarctic filamentous (Stigeoclonium sp.) and coccal alga (Diplosphaera chodatii) on agar plates |
title_short |
The effect of shock freezing on physiological properties and consequent growth of Antarctic filamentous (Stigeoclonium sp.) and coccal alga (Diplosphaera chodatii) on agar plates |
title_full |
The effect of shock freezing on physiological properties and consequent growth of Antarctic filamentous (Stigeoclonium sp.) and coccal alga (Diplosphaera chodatii) on agar plates |
title_fullStr |
The effect of shock freezing on physiological properties and consequent growth of Antarctic filamentous (Stigeoclonium sp.) and coccal alga (Diplosphaera chodatii) on agar plates |
title_full_unstemmed |
The effect of shock freezing on physiological properties and consequent growth of Antarctic filamentous (Stigeoclonium sp.) and coccal alga (Diplosphaera chodatii) on agar plates |
title_sort |
effect of shock freezing on physiological properties and consequent growth of antarctic filamentous (stigeoclonium sp.) and coccal alga (diplosphaera chodatii) on agar plates |
publisher |
Masaryk Univerzity |
publishDate |
2019 |
url |
http://journals.muni.cz/CPR/article/view/12939 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_source |
Czech Polar Reports; Vol 9 No 1 (2019); 37-48 Czech Polar Reports; Vol. 9 No. 1 (2019); 37-48 1805-0697 1805-0689 |
op_relation |
http://journals.muni.cz/CPR/article/view/12939/11291 http://journals.muni.cz/CPR/article/view/12939 |
op_rights |
Copyright (c) 2020 Czech Polar Reports https://creativecommons.org/licenses/by-nc-nd/4.0 |
op_rightsnorm |
CC-BY-NC-ND |
_version_ |
1766245081116835840 |