Antimicrobial activity of microfungi from maritime Antarctic soil
The search for cold-adapted and cold-active fungi in extreme environments provides the potential for discovering new species and novel bioactive compounds. In this study, soil samples were collected from Deception Island, Wilhelmina Bay (north-west Antarctic Peninsula, Graham Land) and Yankee Bay (G...
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ftmasarykunivojs:oai:ojs.journals.muni.cz:article/12893 2023-05-15T13:46:41+02:00 Antimicrobial activity of microfungi from maritime Antarctic soil Abneuf, Mohammed A. Krishnan, Abiramy Aravena, Marcelo Gonzalez Pang, Ka-Lai Convey, Peter Mohamad-Fauzi, Nuradilla Rizman-Idid, Mohammed Alias, Siti Aisyah 2016-07-01 application/pdf http://journals.muni.cz/CPR/article/view/12893 eng eng Masaryk Univerzity http://journals.muni.cz/CPR/article/view/12893/11306 http://journals.muni.cz/CPR/article/view/12893 Copyright (c) 2020 Czech Polar Reports https://creativecommons.org/licenses/by-nc-nd/4.0 CC-BY-NC-ND Czech Polar Reports; Vol 6 No 2 (2016); 141-154 Czech Polar Reports; Vol. 6 No. 2 (2016); 141-154 1805-0697 1805-0689 biodiversity soil fungi isolation antibacterial antifungal info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2016 ftmasarykunivojs 2022-06-26T10:16:39Z The search for cold-adapted and cold-active fungi in extreme environments provides the potential for discovering new species and novel bioactive compounds. In this study, soil samples were collected from Deception Island, Wilhelmina Bay (north-west Antarctic Peninsula, Graham Land) and Yankee Bay (Greenwich Island), maritime Antarctica, for the isolation of soil fungi and determination of their antimicrobial activity. The soil-plate method, agar block, disc diffusion and broth micro-dilution assays were applied to characterize the thermal classes and antimicrobial activity of the isolated fungi. A total of 27 isolates of fungi were obtained from 14 soil samples, including 13 Ascomycota, 4 Zygomycota and 10 anamorphic fungi. Cold-active (psychrotolerant) fungi predominated over cold-adapted (psychrophilic) fungi. In the antimicrobial assay, 16 isolates showed substantial inhibitory activity against test bacterial pathogens. Ethyl acetate extracts of 10 competent isolates showed significant inhibition of bacterial pathogens. Antifungal activity was observed in the disc diffusion assay, but not in the agar block assay. Minimum inhibitory, bactericidal and fungicidal concentrations were determined using the broth micro-dilution method, with an average in the range of 0.78-25 mg ml-1 on the test microorganisms. Isolate WHB-sp. 7 showed the best broad spectrum antimicrobial activity, with the potential for biotechnological studies in antibiotic development. Article in Journal/Newspaper Antarc* Antarctic Antarctic Peninsula Antarctica Deception Island Graham Land Greenwich Island Masaryk University Journals Antarctic Antarctic Peninsula Greenwich Deception Island ENVELOPE(-60.633,-60.633,-62.950,-62.950) Graham Land ENVELOPE(-63.500,-63.500,-66.000,-66.000) Yankee ENVELOPE(-59.769,-59.769,-62.526,-62.526) Wilhelmina ENVELOPE(-62.160,-62.160,-64.642,-64.642) Greenwich Island ENVELOPE(-59.783,-59.783,-62.517,-62.517) Wilhelmina Bay ENVELOPE(-62.160,-62.160,-64.642,-64.642) |
institution |
Open Polar |
collection |
Masaryk University Journals |
op_collection_id |
ftmasarykunivojs |
language |
English |
topic |
biodiversity soil fungi isolation antibacterial antifungal |
spellingShingle |
biodiversity soil fungi isolation antibacterial antifungal Abneuf, Mohammed A. Krishnan, Abiramy Aravena, Marcelo Gonzalez Pang, Ka-Lai Convey, Peter Mohamad-Fauzi, Nuradilla Rizman-Idid, Mohammed Alias, Siti Aisyah Antimicrobial activity of microfungi from maritime Antarctic soil |
topic_facet |
biodiversity soil fungi isolation antibacterial antifungal |
description |
The search for cold-adapted and cold-active fungi in extreme environments provides the potential for discovering new species and novel bioactive compounds. In this study, soil samples were collected from Deception Island, Wilhelmina Bay (north-west Antarctic Peninsula, Graham Land) and Yankee Bay (Greenwich Island), maritime Antarctica, for the isolation of soil fungi and determination of their antimicrobial activity. The soil-plate method, agar block, disc diffusion and broth micro-dilution assays were applied to characterize the thermal classes and antimicrobial activity of the isolated fungi. A total of 27 isolates of fungi were obtained from 14 soil samples, including 13 Ascomycota, 4 Zygomycota and 10 anamorphic fungi. Cold-active (psychrotolerant) fungi predominated over cold-adapted (psychrophilic) fungi. In the antimicrobial assay, 16 isolates showed substantial inhibitory activity against test bacterial pathogens. Ethyl acetate extracts of 10 competent isolates showed significant inhibition of bacterial pathogens. Antifungal activity was observed in the disc diffusion assay, but not in the agar block assay. Minimum inhibitory, bactericidal and fungicidal concentrations were determined using the broth micro-dilution method, with an average in the range of 0.78-25 mg ml-1 on the test microorganisms. Isolate WHB-sp. 7 showed the best broad spectrum antimicrobial activity, with the potential for biotechnological studies in antibiotic development. |
format |
Article in Journal/Newspaper |
author |
Abneuf, Mohammed A. Krishnan, Abiramy Aravena, Marcelo Gonzalez Pang, Ka-Lai Convey, Peter Mohamad-Fauzi, Nuradilla Rizman-Idid, Mohammed Alias, Siti Aisyah |
author_facet |
Abneuf, Mohammed A. Krishnan, Abiramy Aravena, Marcelo Gonzalez Pang, Ka-Lai Convey, Peter Mohamad-Fauzi, Nuradilla Rizman-Idid, Mohammed Alias, Siti Aisyah |
author_sort |
Abneuf, Mohammed A. |
title |
Antimicrobial activity of microfungi from maritime Antarctic soil |
title_short |
Antimicrobial activity of microfungi from maritime Antarctic soil |
title_full |
Antimicrobial activity of microfungi from maritime Antarctic soil |
title_fullStr |
Antimicrobial activity of microfungi from maritime Antarctic soil |
title_full_unstemmed |
Antimicrobial activity of microfungi from maritime Antarctic soil |
title_sort |
antimicrobial activity of microfungi from maritime antarctic soil |
publisher |
Masaryk Univerzity |
publishDate |
2016 |
url |
http://journals.muni.cz/CPR/article/view/12893 |
long_lat |
ENVELOPE(-60.633,-60.633,-62.950,-62.950) ENVELOPE(-63.500,-63.500,-66.000,-66.000) ENVELOPE(-59.769,-59.769,-62.526,-62.526) ENVELOPE(-62.160,-62.160,-64.642,-64.642) ENVELOPE(-59.783,-59.783,-62.517,-62.517) ENVELOPE(-62.160,-62.160,-64.642,-64.642) |
geographic |
Antarctic Antarctic Peninsula Greenwich Deception Island Graham Land Yankee Wilhelmina Greenwich Island Wilhelmina Bay |
geographic_facet |
Antarctic Antarctic Peninsula Greenwich Deception Island Graham Land Yankee Wilhelmina Greenwich Island Wilhelmina Bay |
genre |
Antarc* Antarctic Antarctic Peninsula Antarctica Deception Island Graham Land Greenwich Island |
genre_facet |
Antarc* Antarctic Antarctic Peninsula Antarctica Deception Island Graham Land Greenwich Island |
op_source |
Czech Polar Reports; Vol 6 No 2 (2016); 141-154 Czech Polar Reports; Vol. 6 No. 2 (2016); 141-154 1805-0697 1805-0689 |
op_relation |
http://journals.muni.cz/CPR/article/view/12893/11306 http://journals.muni.cz/CPR/article/view/12893 |
op_rights |
Copyright (c) 2020 Czech Polar Reports https://creativecommons.org/licenses/by-nc-nd/4.0 |
op_rightsnorm |
CC-BY-NC-ND |
_version_ |
1766245075801604096 |