High prevalence and genetic diversity of Treponema paraluisleporidarum isolates in European lagomorphs
The bacterium Treponema paraluisleporidarum causes syphilis in lagomorphs. In a set of 1,095 samples from four species—European brown hare, mountain hare, Corsican hare, and European rabbit—we tested for infection and genotyped the strains that infect wild lagomorphs. Samples originate from Sweden,...
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AMER SOC MICROBIOLOGY
2023
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Online Access: | https://is.muni.cz/publication/2355283 https://doi.org/10.1128/spectrum.01774-23 |
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ftmasarykis:oai:is.muni.cz:2355283 2024-02-04T10:02:07+01:00 High prevalence and genetic diversity of Treponema paraluisleporidarum isolates in European lagomorphs Knauf Sascha Hisgen Linda Ågren Erik O. Barlow Alexander M Faehndrich Marcus Voigt Ulrich Fischer Luisa Grillová Linda Hallmaier-Wacker Luisa K Kik Marja J L Klink Jana C Křenová Jitka Lavazza Antonio Lüert Simone Nováková Markéta Čejková Darina Pacioni Carlo Trogu Tiziana Šmajs David Roos Christian 2023 https://is.muni.cz/publication/2355283 https://doi.org/10.1128/spectrum.01774-23 eng eng AMER SOC MICROBIOLOGY https://is.muni.cz/publication/2355283 info:eu-repo/semantics/openAccess Microbiology spectrum info:eu-repo/semantics/article J 2023 ftmasarykis https://doi.org/10.1128/spectrum.01774-23 2024-01-09T00:27:42Z The bacterium Treponema paraluisleporidarum causes syphilis in lagomorphs. In a set of 1,095 samples from four species—European brown hare, mountain hare, Corsican hare, and European rabbit—we tested for infection and genotyped the strains that infect wild lagomorphs. Samples originate from Sweden, the Netherlands, the United Kingdom, Germany, the Czech Republic, and Italy. The phylogenetic analyses of two informative gene targets (tp0488 and tp0548) showed high genetic diversity among the lagomorph-infecting treponemes. More specifically, we found a high number of nucleotide variants and various short repeat units in the tp0548 locus that have not been described for human syphilis and primate yaws causing Treponema pallidum. While the functional aspect of these short repeat units remains subject to ongoing investigations, it likely enables the pathogen to better survive in its lagomorph host. Our data did not support any geographic clustering, which is equally reflected in the host population genetics as shown by mitochondrial genome data corresponding to the sampled lagomorph populations. This is unexpected and in contrast with what has been shown for nonhuman primate infection with T. pallidum. In the future, the combination of multi-locus sequence typing and whole genome data from modern and ancient samples from a wide geographic range and multiple lagomorph species will contribute to a better understanding of the epidemiology and evolutionary path of lagomorph-infecting treponemes. In conclusion, our current study demonstrates widespread infection and a high genetic variation of the syphilis-causing pathogen in a higher number of positively PCR-tested European lagomorphs (n = 302/1,095). Article in Journal/Newspaper mountain hare Masaryk University: Open Services of Information System Hare Mountain ENVELOPE(-18.767,-18.767,76.767,76.767) Microbiology Spectrum 12 1 |
institution |
Open Polar |
collection |
Masaryk University: Open Services of Information System |
op_collection_id |
ftmasarykis |
language |
English |
description |
The bacterium Treponema paraluisleporidarum causes syphilis in lagomorphs. In a set of 1,095 samples from four species—European brown hare, mountain hare, Corsican hare, and European rabbit—we tested for infection and genotyped the strains that infect wild lagomorphs. Samples originate from Sweden, the Netherlands, the United Kingdom, Germany, the Czech Republic, and Italy. The phylogenetic analyses of two informative gene targets (tp0488 and tp0548) showed high genetic diversity among the lagomorph-infecting treponemes. More specifically, we found a high number of nucleotide variants and various short repeat units in the tp0548 locus that have not been described for human syphilis and primate yaws causing Treponema pallidum. While the functional aspect of these short repeat units remains subject to ongoing investigations, it likely enables the pathogen to better survive in its lagomorph host. Our data did not support any geographic clustering, which is equally reflected in the host population genetics as shown by mitochondrial genome data corresponding to the sampled lagomorph populations. This is unexpected and in contrast with what has been shown for nonhuman primate infection with T. pallidum. In the future, the combination of multi-locus sequence typing and whole genome data from modern and ancient samples from a wide geographic range and multiple lagomorph species will contribute to a better understanding of the epidemiology and evolutionary path of lagomorph-infecting treponemes. In conclusion, our current study demonstrates widespread infection and a high genetic variation of the syphilis-causing pathogen in a higher number of positively PCR-tested European lagomorphs (n = 302/1,095). |
format |
Article in Journal/Newspaper |
author |
Knauf Sascha Hisgen Linda Ågren Erik O. Barlow Alexander M Faehndrich Marcus Voigt Ulrich Fischer Luisa Grillová Linda Hallmaier-Wacker Luisa K Kik Marja J L Klink Jana C Křenová Jitka Lavazza Antonio Lüert Simone Nováková Markéta Čejková Darina Pacioni Carlo Trogu Tiziana Šmajs David Roos Christian |
spellingShingle |
Knauf Sascha Hisgen Linda Ågren Erik O. Barlow Alexander M Faehndrich Marcus Voigt Ulrich Fischer Luisa Grillová Linda Hallmaier-Wacker Luisa K Kik Marja J L Klink Jana C Křenová Jitka Lavazza Antonio Lüert Simone Nováková Markéta Čejková Darina Pacioni Carlo Trogu Tiziana Šmajs David Roos Christian High prevalence and genetic diversity of Treponema paraluisleporidarum isolates in European lagomorphs |
author_facet |
Knauf Sascha Hisgen Linda Ågren Erik O. Barlow Alexander M Faehndrich Marcus Voigt Ulrich Fischer Luisa Grillová Linda Hallmaier-Wacker Luisa K Kik Marja J L Klink Jana C Křenová Jitka Lavazza Antonio Lüert Simone Nováková Markéta Čejková Darina Pacioni Carlo Trogu Tiziana Šmajs David Roos Christian |
author_sort |
Knauf Sascha |
title |
High prevalence and genetic diversity of Treponema paraluisleporidarum isolates in European lagomorphs |
title_short |
High prevalence and genetic diversity of Treponema paraluisleporidarum isolates in European lagomorphs |
title_full |
High prevalence and genetic diversity of Treponema paraluisleporidarum isolates in European lagomorphs |
title_fullStr |
High prevalence and genetic diversity of Treponema paraluisleporidarum isolates in European lagomorphs |
title_full_unstemmed |
High prevalence and genetic diversity of Treponema paraluisleporidarum isolates in European lagomorphs |
title_sort |
high prevalence and genetic diversity of treponema paraluisleporidarum isolates in european lagomorphs |
publisher |
AMER SOC MICROBIOLOGY |
publishDate |
2023 |
url |
https://is.muni.cz/publication/2355283 https://doi.org/10.1128/spectrum.01774-23 |
long_lat |
ENVELOPE(-18.767,-18.767,76.767,76.767) |
geographic |
Hare Mountain |
geographic_facet |
Hare Mountain |
genre |
mountain hare |
genre_facet |
mountain hare |
op_source |
Microbiology spectrum |
op_relation |
https://is.muni.cz/publication/2355283 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1128/spectrum.01774-23 |
container_title |
Microbiology Spectrum |
container_volume |
12 |
container_issue |
1 |
_version_ |
1789968494477967360 |