Evaluation of Non-destructive Molecular Diagnostics for the Detection of Neoparamoeba perurans
Peer reviewed paper. Citation: Downes, J. K., Rigby, M. L., Taylor, R. S., Maynard, B. T., MacCarthy, E., O’Connor, I., Marcos-Lopez M., Rodger H. D., Collins E., Ruane N. M. & Cook, M. T. (2017). Evaluation of Non-destructive Molecular Diagnostics for the Detection of Neoparamoeba perurans. Fro...
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Online Access: | http://hdl.handle.net/10793/1417 https://doi.org/10.3389/fmars.2017.00061 |
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ftmarineinst:oai:oar.marine.ie:10793/1417 2023-05-15T15:32:01+02:00 Evaluation of Non-destructive Molecular Diagnostics for the Detection of Neoparamoeba perurans Downes, Jamie K. Rigby, Megan L. Taylor, Richard S. Maynard, Ben T. MacCarthy, Eugene O'Connor, Ian Marcos-Lopez, Mar Rodger, Hamish D. Collins, Evelyn Ruane, Neil M. Cook, Mathew T. 2017 http://hdl.handle.net/10793/1417 https://doi.org/10.3389/fmars.2017.00061 en eng Frontiers Media SA Frontiers in Marine Science;4 (61) Downes, J. K., Rigby, M. L., Taylor, R. S., Maynard, B. T., MacCarthy, E., O’Connor, I., Marcos-Lopez M., Rodger H. D., Collins E., Ruane N. M. & Cook, M. T. (2017). Evaluation of Non-destructive Molecular Diagnostics for the Detection of Neoparamoeba perurans. Frontiers in Marine Science, 4 (61). https://doi.org/10.3389/fmars.2017.00061 2296-7745 http://hdl.handle.net/10793/1417 doi:10.3389/fmars.2017.00061 4 Atlantic salmon amoebic gill disease Neoparamoeba perurans molecular diagnostics method validation Article 2017 ftmarineinst https://doi.org/10.3389/fmars.2017.00061 2022-07-27T09:39:15Z Peer reviewed paper. Citation: Downes, J. K., Rigby, M. L., Taylor, R. S., Maynard, B. T., MacCarthy, E., O’Connor, I., Marcos-Lopez M., Rodger H. D., Collins E., Ruane N. M. & Cook, M. T. (2017). Evaluation of Non-destructive Molecular Diagnostics for the Detection of Neoparamoeba perurans. Frontiers in Marine Science, 4. https://doi.org/10.3389/fmars.2017.00061 Link: https://www.frontiersin.org/articles/10.3389/fmars.2017.00061/full DOI: https://doi.org/10.3389/fmars.2017.00061 Cited as per the open access policy of Frontiers Media SA. Amoebic gill disease (AGD) caused by Neoparamoeba perurans, has emerged in Europe as a significant problem for the Atlantic salmon farming industry. Gross gill score is the most widely used and practical method for determining AGD severity on farms and informing management decisions on disease mitigation strategies. As molecular diagnosis of AGD remains a high priority for much of the international salmon farming industry, there is a need to evaluate the suitability of currently available molecular assays in conjunction with the most appropriate non-destructive sampling methodology. The aims of this study were to assess a non-destructive sampling methodology (gill swabs) and to compare a range of currently available real-time polymerase chain-reaction (PCR) assays for the detection of N. perurans. Furthermore a comparison of the non-destructive molecular diagnostics with traditional screening methods of gill scoring and histopathology was also undertaken. The study found that all molecular protocols assessed performed well in cases of clinical AGD with high gill scores. A TaqMan based assay (protocol 1) was the optimal assay based on a range of parameters including % positive samples from a field trial performed on fish with gill scores ranging from 0 to 5. A higher proportion of gill swab samples tested positive by all protocols than gill filament biopsies and there was a strong correlation between gill swabs tested by protocol 1 and gross gill score and histology scores. ... Article in Journal/Newspaper Atlantic salmon Marine Institute Open Access Repository Marcos ENVELOPE(-61.833,-61.833,-64.500,-64.500) Lopez ENVELOPE(-63.567,-63.567,-64.850,-64.850) Rigby ENVELOPE(-154.583,-154.583,-85.550,-85.550) Rodger ENVELOPE(-83.567,-83.567,-79.700,-79.700) Frontiers in Marine Science 4 |
institution |
Open Polar |
collection |
Marine Institute Open Access Repository |
op_collection_id |
ftmarineinst |
language |
English |
topic |
Atlantic salmon amoebic gill disease Neoparamoeba perurans molecular diagnostics method validation |
spellingShingle |
Atlantic salmon amoebic gill disease Neoparamoeba perurans molecular diagnostics method validation Downes, Jamie K. Rigby, Megan L. Taylor, Richard S. Maynard, Ben T. MacCarthy, Eugene O'Connor, Ian Marcos-Lopez, Mar Rodger, Hamish D. Collins, Evelyn Ruane, Neil M. Cook, Mathew T. Evaluation of Non-destructive Molecular Diagnostics for the Detection of Neoparamoeba perurans |
topic_facet |
Atlantic salmon amoebic gill disease Neoparamoeba perurans molecular diagnostics method validation |
description |
Peer reviewed paper. Citation: Downes, J. K., Rigby, M. L., Taylor, R. S., Maynard, B. T., MacCarthy, E., O’Connor, I., Marcos-Lopez M., Rodger H. D., Collins E., Ruane N. M. & Cook, M. T. (2017). Evaluation of Non-destructive Molecular Diagnostics for the Detection of Neoparamoeba perurans. Frontiers in Marine Science, 4. https://doi.org/10.3389/fmars.2017.00061 Link: https://www.frontiersin.org/articles/10.3389/fmars.2017.00061/full DOI: https://doi.org/10.3389/fmars.2017.00061 Cited as per the open access policy of Frontiers Media SA. Amoebic gill disease (AGD) caused by Neoparamoeba perurans, has emerged in Europe as a significant problem for the Atlantic salmon farming industry. Gross gill score is the most widely used and practical method for determining AGD severity on farms and informing management decisions on disease mitigation strategies. As molecular diagnosis of AGD remains a high priority for much of the international salmon farming industry, there is a need to evaluate the suitability of currently available molecular assays in conjunction with the most appropriate non-destructive sampling methodology. The aims of this study were to assess a non-destructive sampling methodology (gill swabs) and to compare a range of currently available real-time polymerase chain-reaction (PCR) assays for the detection of N. perurans. Furthermore a comparison of the non-destructive molecular diagnostics with traditional screening methods of gill scoring and histopathology was also undertaken. The study found that all molecular protocols assessed performed well in cases of clinical AGD with high gill scores. A TaqMan based assay (protocol 1) was the optimal assay based on a range of parameters including % positive samples from a field trial performed on fish with gill scores ranging from 0 to 5. A higher proportion of gill swab samples tested positive by all protocols than gill filament biopsies and there was a strong correlation between gill swabs tested by protocol 1 and gross gill score and histology scores. ... |
format |
Article in Journal/Newspaper |
author |
Downes, Jamie K. Rigby, Megan L. Taylor, Richard S. Maynard, Ben T. MacCarthy, Eugene O'Connor, Ian Marcos-Lopez, Mar Rodger, Hamish D. Collins, Evelyn Ruane, Neil M. Cook, Mathew T. |
author_facet |
Downes, Jamie K. Rigby, Megan L. Taylor, Richard S. Maynard, Ben T. MacCarthy, Eugene O'Connor, Ian Marcos-Lopez, Mar Rodger, Hamish D. Collins, Evelyn Ruane, Neil M. Cook, Mathew T. |
author_sort |
Downes, Jamie K. |
title |
Evaluation of Non-destructive Molecular Diagnostics for the Detection of Neoparamoeba perurans |
title_short |
Evaluation of Non-destructive Molecular Diagnostics for the Detection of Neoparamoeba perurans |
title_full |
Evaluation of Non-destructive Molecular Diagnostics for the Detection of Neoparamoeba perurans |
title_fullStr |
Evaluation of Non-destructive Molecular Diagnostics for the Detection of Neoparamoeba perurans |
title_full_unstemmed |
Evaluation of Non-destructive Molecular Diagnostics for the Detection of Neoparamoeba perurans |
title_sort |
evaluation of non-destructive molecular diagnostics for the detection of neoparamoeba perurans |
publisher |
Frontiers Media SA |
publishDate |
2017 |
url |
http://hdl.handle.net/10793/1417 https://doi.org/10.3389/fmars.2017.00061 |
long_lat |
ENVELOPE(-61.833,-61.833,-64.500,-64.500) ENVELOPE(-63.567,-63.567,-64.850,-64.850) ENVELOPE(-154.583,-154.583,-85.550,-85.550) ENVELOPE(-83.567,-83.567,-79.700,-79.700) |
geographic |
Marcos Lopez Rigby Rodger |
geographic_facet |
Marcos Lopez Rigby Rodger |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
4 |
op_relation |
Frontiers in Marine Science;4 (61) Downes, J. K., Rigby, M. L., Taylor, R. S., Maynard, B. T., MacCarthy, E., O’Connor, I., Marcos-Lopez M., Rodger H. D., Collins E., Ruane N. M. & Cook, M. T. (2017). Evaluation of Non-destructive Molecular Diagnostics for the Detection of Neoparamoeba perurans. Frontiers in Marine Science, 4 (61). https://doi.org/10.3389/fmars.2017.00061 2296-7745 http://hdl.handle.net/10793/1417 doi:10.3389/fmars.2017.00061 |
op_doi |
https://doi.org/10.3389/fmars.2017.00061 |
container_title |
Frontiers in Marine Science |
container_volume |
4 |
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1766362514600230912 |