Retrieval of phytoplankton pigments from underway spectrophotometry in the Fram Strait
Phytoplankton in the ocean are extremely diverse. The abundance of various intracellular pigments are often used to study phytoplankton physiology and ecology, and identify and quantify different phytoplankton groups. In this study, phytoplankton absorption spectra (a ph (λ)) derived from underway f...
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ftmaineuniv:oai:digitalcommons.library.umaine.edu:sms_facpub-1234 2023-05-15T16:18:06+02:00 Retrieval of phytoplankton pigments from underway spectrophotometry in the Fram Strait Liu, Yangyang Boss, Emmanuel Chase, Alison Xi, Hongyan Zhang, Xiaodong Röttgers, Rüdiger Pan, Yanqun Bracher, Astrid 2019-02-01T08:00:00Z application/pdf https://digitalcommons.library.umaine.edu/sms_facpub/235 https://digitalcommons.library.umaine.edu/cgi/viewcontent.cgi?article=1234&context=sms_facpub unknown DigitalCommons@UMaine https://digitalcommons.library.umaine.edu/sms_facpub/235 https://digitalcommons.library.umaine.edu/cgi/viewcontent.cgi?article=1234&context=sms_facpub This Item is protected by copyright and/or related rights. You are free to use this item in any way that is permitted by copyright and related rights legislation that applies to your use. Rights assessment remains the responsibility of the researcher. In addition, no permission is required from the rights-holder(s) for non-commercial uses. Marine Sciences Faculty Scholarship Gaussian decomposition Matrix inversion Non-negative least squares Phytoplankton absorption Phytoplankton pigments Singular value decomposition Underway AC-S flow-through system Oceanography and Atmospheric Sciences and Meteorology text 2019 ftmaineuniv 2023-03-12T20:12:28Z Phytoplankton in the ocean are extremely diverse. The abundance of various intracellular pigments are often used to study phytoplankton physiology and ecology, and identify and quantify different phytoplankton groups. In this study, phytoplankton absorption spectra (a ph (λ)) derived from underway flow-through AC-S measurements in the Fram Strait are combined with phytoplankton pigment measurements analyzed by high-performance liquid chromatography (HPLC) to evaluate the retrieval of various pigment concentrations at high spatial resolution. The performances of two approaches, Gaussian decomposition and the matrix inversion technique are investigated and compared. Our study is the first to apply the matrix inversion technique to underway spectrophotometry data. We find that Gaussian decomposition provides good estimates (median absolute percentage error, MPE 21-34%) of total chlorophyll-a (TChl-a), total chlorophyll-b (TChl-b), the combination of chlorophyll-c1 and -c2 (Chl-c1/2), photoprotective (PPC) and photosynthetic carotenoids (PSC). This method outperformed one of the matrix inversion algorithms, i.e., singular value decomposition combined with non-negative least squares (SVD-NNLS), in retrieving TChl-b, Chl-c1/2, PSC, and PPC. However, SVD-NNLS enables robust retrievals of specific carotenoids (MPE 37-65%), i.e., fucoxanthin, diadinoxanthin and 19'-hexanoyloxyfucoxanthin, which is currently not accomplished by Gaussian decomposition. More robust predictions are obtained using the Gaussian decomposition method when the observed a ph (λ) is normalized by the package effect index at 675 nm. The latter is determined as a function of "packaged" a ph (675) and TChl-a concentration, which shows potential for improving pigment retrieval accuracy by the combined use of a ph (λ) and TChl-a concentration data. To generate robust estimation statistics for the matrix inversion technique, we combine leave-one-out cross-validation with data perturbations.We find that both approaches provide useful information on ... Text Fram Strait The University of Maine: DigitalCommons@UMaine |
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Open Polar |
collection |
The University of Maine: DigitalCommons@UMaine |
op_collection_id |
ftmaineuniv |
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unknown |
topic |
Gaussian decomposition Matrix inversion Non-negative least squares Phytoplankton absorption Phytoplankton pigments Singular value decomposition Underway AC-S flow-through system Oceanography and Atmospheric Sciences and Meteorology |
spellingShingle |
Gaussian decomposition Matrix inversion Non-negative least squares Phytoplankton absorption Phytoplankton pigments Singular value decomposition Underway AC-S flow-through system Oceanography and Atmospheric Sciences and Meteorology Liu, Yangyang Boss, Emmanuel Chase, Alison Xi, Hongyan Zhang, Xiaodong Röttgers, Rüdiger Pan, Yanqun Bracher, Astrid Retrieval of phytoplankton pigments from underway spectrophotometry in the Fram Strait |
topic_facet |
Gaussian decomposition Matrix inversion Non-negative least squares Phytoplankton absorption Phytoplankton pigments Singular value decomposition Underway AC-S flow-through system Oceanography and Atmospheric Sciences and Meteorology |
description |
Phytoplankton in the ocean are extremely diverse. The abundance of various intracellular pigments are often used to study phytoplankton physiology and ecology, and identify and quantify different phytoplankton groups. In this study, phytoplankton absorption spectra (a ph (λ)) derived from underway flow-through AC-S measurements in the Fram Strait are combined with phytoplankton pigment measurements analyzed by high-performance liquid chromatography (HPLC) to evaluate the retrieval of various pigment concentrations at high spatial resolution. The performances of two approaches, Gaussian decomposition and the matrix inversion technique are investigated and compared. Our study is the first to apply the matrix inversion technique to underway spectrophotometry data. We find that Gaussian decomposition provides good estimates (median absolute percentage error, MPE 21-34%) of total chlorophyll-a (TChl-a), total chlorophyll-b (TChl-b), the combination of chlorophyll-c1 and -c2 (Chl-c1/2), photoprotective (PPC) and photosynthetic carotenoids (PSC). This method outperformed one of the matrix inversion algorithms, i.e., singular value decomposition combined with non-negative least squares (SVD-NNLS), in retrieving TChl-b, Chl-c1/2, PSC, and PPC. However, SVD-NNLS enables robust retrievals of specific carotenoids (MPE 37-65%), i.e., fucoxanthin, diadinoxanthin and 19'-hexanoyloxyfucoxanthin, which is currently not accomplished by Gaussian decomposition. More robust predictions are obtained using the Gaussian decomposition method when the observed a ph (λ) is normalized by the package effect index at 675 nm. The latter is determined as a function of "packaged" a ph (675) and TChl-a concentration, which shows potential for improving pigment retrieval accuracy by the combined use of a ph (λ) and TChl-a concentration data. To generate robust estimation statistics for the matrix inversion technique, we combine leave-one-out cross-validation with data perturbations.We find that both approaches provide useful information on ... |
format |
Text |
author |
Liu, Yangyang Boss, Emmanuel Chase, Alison Xi, Hongyan Zhang, Xiaodong Röttgers, Rüdiger Pan, Yanqun Bracher, Astrid |
author_facet |
Liu, Yangyang Boss, Emmanuel Chase, Alison Xi, Hongyan Zhang, Xiaodong Röttgers, Rüdiger Pan, Yanqun Bracher, Astrid |
author_sort |
Liu, Yangyang |
title |
Retrieval of phytoplankton pigments from underway spectrophotometry in the Fram Strait |
title_short |
Retrieval of phytoplankton pigments from underway spectrophotometry in the Fram Strait |
title_full |
Retrieval of phytoplankton pigments from underway spectrophotometry in the Fram Strait |
title_fullStr |
Retrieval of phytoplankton pigments from underway spectrophotometry in the Fram Strait |
title_full_unstemmed |
Retrieval of phytoplankton pigments from underway spectrophotometry in the Fram Strait |
title_sort |
retrieval of phytoplankton pigments from underway spectrophotometry in the fram strait |
publisher |
DigitalCommons@UMaine |
publishDate |
2019 |
url |
https://digitalcommons.library.umaine.edu/sms_facpub/235 https://digitalcommons.library.umaine.edu/cgi/viewcontent.cgi?article=1234&context=sms_facpub |
genre |
Fram Strait |
genre_facet |
Fram Strait |
op_source |
Marine Sciences Faculty Scholarship |
op_relation |
https://digitalcommons.library.umaine.edu/sms_facpub/235 https://digitalcommons.library.umaine.edu/cgi/viewcontent.cgi?article=1234&context=sms_facpub |
op_rights |
This Item is protected by copyright and/or related rights. You are free to use this item in any way that is permitted by copyright and related rights legislation that applies to your use. Rights assessment remains the responsibility of the researcher. In addition, no permission is required from the rights-holder(s) for non-commercial uses. |
_version_ |
1766004220683616256 |