Heterogeneity of surface attached microbial communities from Sydney Harbour, Australia
There is limited information on bacterial communities attached to marine surfaces. These surface attached bacterial communities can vary at a micro scale and these differences may be due to surface characteristics in marine environments. The current study investigates the heterogeneity of bacterial...
Published in: | Marine Genomics |
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2010
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Online Access: | https://researchers.mq.edu.au/en/publications/a302b8e9-069c-4076-b405-06a0f304432e https://doi.org/10.1016/j.margen.2010.08.001 http://www.scopus.com/inward/record.url?scp=77957780177&partnerID=8YFLogxK |
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ftmacquarieunicr:oai:https://researchers.mq.edu.au:publications/a302b8e9-069c-4076-b405-06a0f304432e 2023-05-15T15:58:35+02:00 Heterogeneity of surface attached microbial communities from Sydney Harbour, Australia Wilson, George S. Raftos, David A. Gillings, Michael R. Nair, Sham V. 2010-06 https://researchers.mq.edu.au/en/publications/a302b8e9-069c-4076-b405-06a0f304432e https://doi.org/10.1016/j.margen.2010.08.001 http://www.scopus.com/inward/record.url?scp=77957780177&partnerID=8YFLogxK eng eng info:eu-repo/semantics/restrictedAccess Wilson , G S , Raftos , D A , Gillings , M R & Nair , S V 2010 , ' Heterogeneity of surface attached microbial communities from Sydney Harbour, Australia ' , Marine Genomics , vol. 3 , no. 2 , pp. 99-105 . https://doi.org/10.1016/j.margen.2010.08.001 article 2010 ftmacquarieunicr https://doi.org/10.1016/j.margen.2010.08.001 2022-11-06T06:38:21Z There is limited information on bacterial communities attached to marine surfaces. These surface attached bacterial communities can vary at a micro scale and these differences may be due to surface characteristics in marine environments. The current study investigates the heterogeneity of bacterial communities on five different marine invertebrates (Heliocidaris erythrogramma, Austrocochlea concamerata, Crassostrea gigas, Dendrilla rosea, and Actinia tenebrosa), the alga, Lobophora variegata and marine gravel from a 20. m × 20. m quadrant in Camp Cove, Sydney Harbour, Australia. Terminal restriction fragment length polymorphism (TRFLP) of 16S sequences showed that each surface contained unique combinations of TRFLP fragment lengths. Phylogenetic analysis of random clones picked from clone libraries constructed from the amplified 16S sequences revealed that 16S sequences from the communities on different surfaces clustered into distinct clades. None of the bacteria identified by 16S sequencing of the whole (uncultured) microbial communities was detected after cultivation. Overall, the study shows surface type plays a major role in shaping microbial communities in marine environments. Article in Journal/Newspaper Crassostrea gigas Macquarie University Research Portal Camp Cove ENVELOPE(-56.765,-56.765,49.883,49.883) Marine Genomics 3 2 99 105 |
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Open Polar |
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Macquarie University Research Portal |
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ftmacquarieunicr |
language |
English |
description |
There is limited information on bacterial communities attached to marine surfaces. These surface attached bacterial communities can vary at a micro scale and these differences may be due to surface characteristics in marine environments. The current study investigates the heterogeneity of bacterial communities on five different marine invertebrates (Heliocidaris erythrogramma, Austrocochlea concamerata, Crassostrea gigas, Dendrilla rosea, and Actinia tenebrosa), the alga, Lobophora variegata and marine gravel from a 20. m × 20. m quadrant in Camp Cove, Sydney Harbour, Australia. Terminal restriction fragment length polymorphism (TRFLP) of 16S sequences showed that each surface contained unique combinations of TRFLP fragment lengths. Phylogenetic analysis of random clones picked from clone libraries constructed from the amplified 16S sequences revealed that 16S sequences from the communities on different surfaces clustered into distinct clades. None of the bacteria identified by 16S sequencing of the whole (uncultured) microbial communities was detected after cultivation. Overall, the study shows surface type plays a major role in shaping microbial communities in marine environments. |
format |
Article in Journal/Newspaper |
author |
Wilson, George S. Raftos, David A. Gillings, Michael R. Nair, Sham V. |
spellingShingle |
Wilson, George S. Raftos, David A. Gillings, Michael R. Nair, Sham V. Heterogeneity of surface attached microbial communities from Sydney Harbour, Australia |
author_facet |
Wilson, George S. Raftos, David A. Gillings, Michael R. Nair, Sham V. |
author_sort |
Wilson, George S. |
title |
Heterogeneity of surface attached microbial communities from Sydney Harbour, Australia |
title_short |
Heterogeneity of surface attached microbial communities from Sydney Harbour, Australia |
title_full |
Heterogeneity of surface attached microbial communities from Sydney Harbour, Australia |
title_fullStr |
Heterogeneity of surface attached microbial communities from Sydney Harbour, Australia |
title_full_unstemmed |
Heterogeneity of surface attached microbial communities from Sydney Harbour, Australia |
title_sort |
heterogeneity of surface attached microbial communities from sydney harbour, australia |
publishDate |
2010 |
url |
https://researchers.mq.edu.au/en/publications/a302b8e9-069c-4076-b405-06a0f304432e https://doi.org/10.1016/j.margen.2010.08.001 http://www.scopus.com/inward/record.url?scp=77957780177&partnerID=8YFLogxK |
long_lat |
ENVELOPE(-56.765,-56.765,49.883,49.883) |
geographic |
Camp Cove |
geographic_facet |
Camp Cove |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Wilson , G S , Raftos , D A , Gillings , M R & Nair , S V 2010 , ' Heterogeneity of surface attached microbial communities from Sydney Harbour, Australia ' , Marine Genomics , vol. 3 , no. 2 , pp. 99-105 . https://doi.org/10.1016/j.margen.2010.08.001 |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1016/j.margen.2010.08.001 |
container_title |
Marine Genomics |
container_volume |
3 |
container_issue |
2 |
container_start_page |
99 |
op_container_end_page |
105 |
_version_ |
1766394354617810944 |