Distinct immune responses of juvenile and adult oysters ( Crassostrea gigas ) to viral and bacterial infections
Since 2008, massive mortality events of Pacific oysters (Crassostrea gigas) have been reported worldwide and these disease events are often associated with Ostreid herpesvirus type 1 (OsHV-1). Epidemiological field studies have also reported oyster age and other pathogens of the Vibrio genus are con...
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ftmacquarieunicr:oai:https://researchers.mq.edu.au:publications/99f2e42a-e9a1-4b83-aaa1-158fad0b621d 2024-06-23T07:52:16+00:00 Distinct immune responses of juvenile and adult oysters ( Crassostrea gigas ) to viral and bacterial infections Green, Timothy J. Vergnes, Agnes Montagnani, Caroline De Lorgeril, Julien 2016-07-21 application/pdf https://researchers.mq.edu.au/en/publications/99f2e42a-e9a1-4b83-aaa1-158fad0b621d https://doi.org/10.1186/s13567-016-0356-7 https://research-management.mq.edu.au/ws/files/62174169/Publisher%20version%20(open%20access).pdf http://www.scopus.com/inward/record.url?scp=84978646258&partnerID=8YFLogxK eng eng info:eu-repo/semantics/openAccess Green , T J , Vergnes , A , Montagnani , C & De Lorgeril , J 2016 , ' Distinct immune responses of juvenile and adult oysters ( Crassostrea gigas ) to viral and bacterial infections ' , Veterinary Research , vol. 47 , 72 , pp. 1-11 . https://doi.org/10.1186/s13567-016-0356-7 article 2016 ftmacquarieunicr https://doi.org/10.1186/s13567-016-0356-7 2024-06-05T23:47:46Z Since 2008, massive mortality events of Pacific oysters (Crassostrea gigas) have been reported worldwide and these disease events are often associated with Ostreid herpesvirus type 1 (OsHV-1). Epidemiological field studies have also reported oyster age and other pathogens of the Vibrio genus are contributing factors to this syndrome. We undertook a controlled laboratory experiment to simultaneously investigate survival and immunological response of juvenile and adult C. gigas at different time-points post-infection with OsHV-1, Vibrio tasmaniensis LGP32 and V. aestuarianus. Our data corroborates epidemiological studies that juveniles are more susceptible to OsHV-1, whereas adults are more susceptible to Vibrio. We measured the expression of 102 immune-genes by high-throughput RT-qPCR, which revealed oysters have different transcriptional responses to OsHV-1 and Vibrio. The transcriptional response in the early stages of OsHV-1 infection involved genes related to apoptosis and the interferon-pathway. Transcriptional response to Vibrio infection involved antimicrobial peptides, heat shock proteins and galectins. Interestingly, oysters in the later stages of OsHV-1 infection had a transcriptional response that resembled an antibacterial response, which is suggestive of the oyster's microbiome causing secondary infections (dysbiosis-driven pathology). This study provides molecular evidence that oysters can mount distinct immune response to viral and bacterial pathogens and these responses differ depending on the age of the host. Article in Journal/Newspaper Crassostrea gigas Macquarie University Research Portal Pacific Veterinary Research 47 1 |
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Macquarie University Research Portal |
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ftmacquarieunicr |
language |
English |
description |
Since 2008, massive mortality events of Pacific oysters (Crassostrea gigas) have been reported worldwide and these disease events are often associated with Ostreid herpesvirus type 1 (OsHV-1). Epidemiological field studies have also reported oyster age and other pathogens of the Vibrio genus are contributing factors to this syndrome. We undertook a controlled laboratory experiment to simultaneously investigate survival and immunological response of juvenile and adult C. gigas at different time-points post-infection with OsHV-1, Vibrio tasmaniensis LGP32 and V. aestuarianus. Our data corroborates epidemiological studies that juveniles are more susceptible to OsHV-1, whereas adults are more susceptible to Vibrio. We measured the expression of 102 immune-genes by high-throughput RT-qPCR, which revealed oysters have different transcriptional responses to OsHV-1 and Vibrio. The transcriptional response in the early stages of OsHV-1 infection involved genes related to apoptosis and the interferon-pathway. Transcriptional response to Vibrio infection involved antimicrobial peptides, heat shock proteins and galectins. Interestingly, oysters in the later stages of OsHV-1 infection had a transcriptional response that resembled an antibacterial response, which is suggestive of the oyster's microbiome causing secondary infections (dysbiosis-driven pathology). This study provides molecular evidence that oysters can mount distinct immune response to viral and bacterial pathogens and these responses differ depending on the age of the host. |
format |
Article in Journal/Newspaper |
author |
Green, Timothy J. Vergnes, Agnes Montagnani, Caroline De Lorgeril, Julien |
spellingShingle |
Green, Timothy J. Vergnes, Agnes Montagnani, Caroline De Lorgeril, Julien Distinct immune responses of juvenile and adult oysters ( Crassostrea gigas ) to viral and bacterial infections |
author_facet |
Green, Timothy J. Vergnes, Agnes Montagnani, Caroline De Lorgeril, Julien |
author_sort |
Green, Timothy J. |
title |
Distinct immune responses of juvenile and adult oysters ( Crassostrea gigas ) to viral and bacterial infections |
title_short |
Distinct immune responses of juvenile and adult oysters ( Crassostrea gigas ) to viral and bacterial infections |
title_full |
Distinct immune responses of juvenile and adult oysters ( Crassostrea gigas ) to viral and bacterial infections |
title_fullStr |
Distinct immune responses of juvenile and adult oysters ( Crassostrea gigas ) to viral and bacterial infections |
title_full_unstemmed |
Distinct immune responses of juvenile and adult oysters ( Crassostrea gigas ) to viral and bacterial infections |
title_sort |
distinct immune responses of juvenile and adult oysters ( crassostrea gigas ) to viral and bacterial infections |
publishDate |
2016 |
url |
https://researchers.mq.edu.au/en/publications/99f2e42a-e9a1-4b83-aaa1-158fad0b621d https://doi.org/10.1186/s13567-016-0356-7 https://research-management.mq.edu.au/ws/files/62174169/Publisher%20version%20(open%20access).pdf http://www.scopus.com/inward/record.url?scp=84978646258&partnerID=8YFLogxK |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Green , T J , Vergnes , A , Montagnani , C & De Lorgeril , J 2016 , ' Distinct immune responses of juvenile and adult oysters ( Crassostrea gigas ) to viral and bacterial infections ' , Veterinary Research , vol. 47 , 72 , pp. 1-11 . https://doi.org/10.1186/s13567-016-0356-7 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1186/s13567-016-0356-7 |
container_title |
Veterinary Research |
container_volume |
47 |
container_issue |
1 |
_version_ |
1802643518669193216 |